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Presence of 2-hydroxymyristate on endotoxins is associated with death in neonates with Enterobacter cloacae complex septic shock

Enterobacter cloacae complex species are involved in infections among critically ill patients. After a recent E.cloacae outbreak of fulminant neonatal septic shock, we conducted a study to determine whether septic shock severity and its lethal consequence are related to structural features of the en...

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Autores principales: Augusto, Luis A., Bourgeois-Nicolaos, Nadège, Breton, Aude, Barreault, Simon, Alonso, Enrique Hernandez, Gera, Stuti, Faraut-Derouin, Véronique, Semaan, Nada, De Luca, Daniele, Chaby, Richard, Doucet-Populaire, Florence, Tissières, Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361193/
https://www.ncbi.nlm.nih.gov/pubmed/34409274
http://dx.doi.org/10.1016/j.isci.2021.102916
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author Augusto, Luis A.
Bourgeois-Nicolaos, Nadège
Breton, Aude
Barreault, Simon
Alonso, Enrique Hernandez
Gera, Stuti
Faraut-Derouin, Véronique
Semaan, Nada
De Luca, Daniele
Chaby, Richard
Doucet-Populaire, Florence
Tissières, Pierre
author_facet Augusto, Luis A.
Bourgeois-Nicolaos, Nadège
Breton, Aude
Barreault, Simon
Alonso, Enrique Hernandez
Gera, Stuti
Faraut-Derouin, Véronique
Semaan, Nada
De Luca, Daniele
Chaby, Richard
Doucet-Populaire, Florence
Tissières, Pierre
author_sort Augusto, Luis A.
collection PubMed
description Enterobacter cloacae complex species are involved in infections among critically ill patients. After a recent E.cloacae outbreak of fulminant neonatal septic shock, we conducted a study to determine whether septic shock severity and its lethal consequence are related to structural features of the endotoxin (lipopolysaccharide [LPS]) of the strains isolated from hospitalized infants and more specifically its lipid A region. It appeared that the LPSs are very heterogeneous, carrying fifteen different molecular species of lipid A. The virulence was correlated with a structural feature identified by matrix-assisted laser desorption ionization–time of flight mass spectrometry and gas chromatography coupled with mass spectrometry: the presence of 2-hydroxymyristic acid as a secondary substituent in lipid A. This is the first published evidence linking LPS structural moiety to neonatal sepsis outcome and opens the possibility of using this fatty acid marker as a detection tool for high-risk patients, which could help reduce their mortality.
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spelling pubmed-83611932021-08-17 Presence of 2-hydroxymyristate on endotoxins is associated with death in neonates with Enterobacter cloacae complex septic shock Augusto, Luis A. Bourgeois-Nicolaos, Nadège Breton, Aude Barreault, Simon Alonso, Enrique Hernandez Gera, Stuti Faraut-Derouin, Véronique Semaan, Nada De Luca, Daniele Chaby, Richard Doucet-Populaire, Florence Tissières, Pierre iScience Article Enterobacter cloacae complex species are involved in infections among critically ill patients. After a recent E.cloacae outbreak of fulminant neonatal septic shock, we conducted a study to determine whether septic shock severity and its lethal consequence are related to structural features of the endotoxin (lipopolysaccharide [LPS]) of the strains isolated from hospitalized infants and more specifically its lipid A region. It appeared that the LPSs are very heterogeneous, carrying fifteen different molecular species of lipid A. The virulence was correlated with a structural feature identified by matrix-assisted laser desorption ionization–time of flight mass spectrometry and gas chromatography coupled with mass spectrometry: the presence of 2-hydroxymyristic acid as a secondary substituent in lipid A. This is the first published evidence linking LPS structural moiety to neonatal sepsis outcome and opens the possibility of using this fatty acid marker as a detection tool for high-risk patients, which could help reduce their mortality. Elsevier 2021-07-30 /pmc/articles/PMC8361193/ /pubmed/34409274 http://dx.doi.org/10.1016/j.isci.2021.102916 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Augusto, Luis A.
Bourgeois-Nicolaos, Nadège
Breton, Aude
Barreault, Simon
Alonso, Enrique Hernandez
Gera, Stuti
Faraut-Derouin, Véronique
Semaan, Nada
De Luca, Daniele
Chaby, Richard
Doucet-Populaire, Florence
Tissières, Pierre
Presence of 2-hydroxymyristate on endotoxins is associated with death in neonates with Enterobacter cloacae complex septic shock
title Presence of 2-hydroxymyristate on endotoxins is associated with death in neonates with Enterobacter cloacae complex septic shock
title_full Presence of 2-hydroxymyristate on endotoxins is associated with death in neonates with Enterobacter cloacae complex septic shock
title_fullStr Presence of 2-hydroxymyristate on endotoxins is associated with death in neonates with Enterobacter cloacae complex septic shock
title_full_unstemmed Presence of 2-hydroxymyristate on endotoxins is associated with death in neonates with Enterobacter cloacae complex septic shock
title_short Presence of 2-hydroxymyristate on endotoxins is associated with death in neonates with Enterobacter cloacae complex septic shock
title_sort presence of 2-hydroxymyristate on endotoxins is associated with death in neonates with enterobacter cloacae complex septic shock
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361193/
https://www.ncbi.nlm.nih.gov/pubmed/34409274
http://dx.doi.org/10.1016/j.isci.2021.102916
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