Luciferase reporter assays to monitor interferon signaling modulation by SARS-CoV-2 proteins

We present a protocol for analyzing the impact of SARS-CoV-2 proteins in interferon signaling using luciferase reporter assays. Here, the induction of defined promoters can be quantitatively assessed with high sensitivity and broad linear range. The results are similar to those obtained using qPCR t...

Descripción completa

Detalles Bibliográficos
Autores principales: Hirschenberger, Maximilian, Hayn, Manuel, Laliberté, Alexandre, Koepke, Lennart, Kirchhoff, Frank, Sparrer, Konstantin Maria Johannes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361205/
https://www.ncbi.nlm.nih.gov/pubmed/34405154
http://dx.doi.org/10.1016/j.xpro.2021.100781

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361205/
https://www.ncbi.nlm.nih.gov/pubmed/34405154
http://dx.doi.org/10.1016/j.xpro.2021.100781

Ejemplares similares