Luciferase reporter assays to monitor interferon signaling modulation by SARS-CoV-2 proteins

We present a protocol for analyzing the impact of SARS-CoV-2 proteins in interferon signaling using luciferase reporter assays. Here, the induction of defined promoters can be quantitatively assessed with high sensitivity and broad linear range. The results are similar to those obtained using qPCR t...

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Detalles Bibliográficos
Autores principales: Hirschenberger, Maximilian, Hayn, Manuel, Laliberté, Alexandre, Koepke, Lennart, Kirchhoff, Frank, Sparrer, Konstantin Maria Johannes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361205/
https://www.ncbi.nlm.nih.gov/pubmed/34405154
http://dx.doi.org/10.1016/j.xpro.2021.100781
Descripción
Sumario:We present a protocol for analyzing the impact of SARS-CoV-2 proteins in interferon signaling using luciferase reporter assays. Here, the induction of defined promoters can be quantitatively assessed with high sensitivity and broad linear range. The results are similar to those obtained using qPCR to measure endogenous mRNA induction. The assay requires stringent normalization and confirmation of the results in more physiological settings. The protocol is adaptable for other viruses and other innate immune stimuli. For complete details on the use and execution of this protocol, please refer to Hayn et al. (2021).

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