Luciferase reporter assays to monitor interferon signaling modulation by SARS-CoV-2 proteins

We present a protocol for analyzing the impact of SARS-CoV-2 proteins in interferon signaling using luciferase reporter assays. Here, the induction of defined promoters can be quantitatively assessed with high sensitivity and broad linear range. The results are similar to those obtained using qPCR t...

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Detalles Bibliográficos
Autores principales: Hirschenberger, Maximilian, Hayn, Manuel, Laliberté, Alexandre, Koepke, Lennart, Kirchhoff, Frank, Sparrer, Konstantin Maria Johannes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361205/
https://www.ncbi.nlm.nih.gov/pubmed/34405154
http://dx.doi.org/10.1016/j.xpro.2021.100781
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author Hirschenberger, Maximilian
Hayn, Manuel
Laliberté, Alexandre
Koepke, Lennart
Kirchhoff, Frank
Sparrer, Konstantin Maria Johannes
author_facet Hirschenberger, Maximilian
Hayn, Manuel
Laliberté, Alexandre
Koepke, Lennart
Kirchhoff, Frank
Sparrer, Konstantin Maria Johannes
author_sort Hirschenberger, Maximilian
collection PubMed
description We present a protocol for analyzing the impact of SARS-CoV-2 proteins in interferon signaling using luciferase reporter assays. Here, the induction of defined promoters can be quantitatively assessed with high sensitivity and broad linear range. The results are similar to those obtained using qPCR to measure endogenous mRNA induction. The assay requires stringent normalization and confirmation of the results in more physiological settings. The protocol is adaptable for other viruses and other innate immune stimuli. For complete details on the use and execution of this protocol, please refer to Hayn et al. (2021).
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spelling pubmed-83612052021-08-13 Luciferase reporter assays to monitor interferon signaling modulation by SARS-CoV-2 proteins Hirschenberger, Maximilian Hayn, Manuel Laliberté, Alexandre Koepke, Lennart Kirchhoff, Frank Sparrer, Konstantin Maria Johannes STAR Protoc Protocol We present a protocol for analyzing the impact of SARS-CoV-2 proteins in interferon signaling using luciferase reporter assays. Here, the induction of defined promoters can be quantitatively assessed with high sensitivity and broad linear range. The results are similar to those obtained using qPCR to measure endogenous mRNA induction. The assay requires stringent normalization and confirmation of the results in more physiological settings. The protocol is adaptable for other viruses and other innate immune stimuli. For complete details on the use and execution of this protocol, please refer to Hayn et al. (2021). Elsevier 2021-08-13 /pmc/articles/PMC8361205/ /pubmed/34405154 http://dx.doi.org/10.1016/j.xpro.2021.100781 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Hirschenberger, Maximilian
Hayn, Manuel
Laliberté, Alexandre
Koepke, Lennart
Kirchhoff, Frank
Sparrer, Konstantin Maria Johannes
Luciferase reporter assays to monitor interferon signaling modulation by SARS-CoV-2 proteins
title Luciferase reporter assays to monitor interferon signaling modulation by SARS-CoV-2 proteins
title_full Luciferase reporter assays to monitor interferon signaling modulation by SARS-CoV-2 proteins
title_fullStr Luciferase reporter assays to monitor interferon signaling modulation by SARS-CoV-2 proteins
title_full_unstemmed Luciferase reporter assays to monitor interferon signaling modulation by SARS-CoV-2 proteins
title_short Luciferase reporter assays to monitor interferon signaling modulation by SARS-CoV-2 proteins
title_sort luciferase reporter assays to monitor interferon signaling modulation by sars-cov-2 proteins
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361205/
https://www.ncbi.nlm.nih.gov/pubmed/34405154
http://dx.doi.org/10.1016/j.xpro.2021.100781
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