Sequence variation in the β7–β8 loop of bacterial class A sortase enzymes alters substrate selectivity

Gram-positive bacteria contain sortase enzymes on their cell surfaces that catalyze transpeptidation reactions critical for proper cellular function. In vitro, sortases are used in sortase-mediated ligation (SML) reactions for a variety of protein engineering applications. Historically, sortase A fr...

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Detalles Bibliográficos
Autores principales: Piper, Isabel M., Struyvenberg, Sarah A., Valgardson, Jordan D., Johnson, D. Alex, Gao, Melody, Johnston, Katherine, Svendsen, Justin E., Kodama, Hanna M., Hvorecny, Kelli L., Antos, John M., Amacher, Jeanine F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361268/
https://www.ncbi.nlm.nih.gov/pubmed/34302812
http://dx.doi.org/10.1016/j.jbc.2021.100981

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