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Multifunctional Heterometallic Ir(III)−Au(I) Probes as Promising Anticancer and Antiangiogenic Agents
A new class of emissive cyclometallated Ir(III)−Au(I) complexes with a bis(diphenylphosphino) methanide bridging ligand was successfully synthesised from the diphosphino complex [Ir(N^C)(2)(dppm)](+) (1). The different gold ancillary ligand, a triphenylphosphine (2), a chloride (3) or a thiocytosine...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361937/ https://www.ncbi.nlm.nih.gov/pubmed/33860585 http://dx.doi.org/10.1002/chem.202100707 |
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author | Redrado, Marta Benedi, Andrea Marzo, Isabel García‐Otín, Angel L. Fernández‐Moreira, Vanesa Concepción Gimeno, M. |
author_facet | Redrado, Marta Benedi, Andrea Marzo, Isabel García‐Otín, Angel L. Fernández‐Moreira, Vanesa Concepción Gimeno, M. |
author_sort | Redrado, Marta |
collection | PubMed |
description | A new class of emissive cyclometallated Ir(III)−Au(I) complexes with a bis(diphenylphosphino) methanide bridging ligand was successfully synthesised from the diphosphino complex [Ir(N^C)(2)(dppm)](+) (1). The different gold ancillary ligand, a triphenylphosphine (2), a chloride (3) or a thiocytosine (4) did not reveal any significant effect on the photophysical properties, which are mainly due to metal‐to‐ligand charge‐transfer ((3)MLCT) transitions based on Ir(III). However, the Au(I) fragment, along with the ancillary ligand, seemed crucial for the bioactivity in A549 lung carcinoma cells versus endothelial cells. Both cell types display variable sensitivities to the complexes (IC(50)=0.6–3.5 μM). The apoptotic pathway is activated in all cases, and paraptotic cell death seems to take place at initial stages in A549 cells. Species 2–4 showed at least dual lysosomal and mitochondrial biodistribution in A549 cells, with an initial lysosomal localisation and a possible trafficking process between both organelles with time. The bimetallic Ir(III)−Au(I) complexes disrupted the mitochondrial transmembrane potential in A549 cells and increased reactive oxygen species (ROS) generation and thioredoxin reductase (TrxR) inhibition in comparison with that displayed by the monometallic complex 1. Angiogenic activity assays performed in endothelial cells revealed the promising antimetastatic potential of 1, 2 and 4. |
format | Online Article Text |
id | pubmed-8361937 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83619372021-08-17 Multifunctional Heterometallic Ir(III)−Au(I) Probes as Promising Anticancer and Antiangiogenic Agents Redrado, Marta Benedi, Andrea Marzo, Isabel García‐Otín, Angel L. Fernández‐Moreira, Vanesa Concepción Gimeno, M. Chemistry Full Papers A new class of emissive cyclometallated Ir(III)−Au(I) complexes with a bis(diphenylphosphino) methanide bridging ligand was successfully synthesised from the diphosphino complex [Ir(N^C)(2)(dppm)](+) (1). The different gold ancillary ligand, a triphenylphosphine (2), a chloride (3) or a thiocytosine (4) did not reveal any significant effect on the photophysical properties, which are mainly due to metal‐to‐ligand charge‐transfer ((3)MLCT) transitions based on Ir(III). However, the Au(I) fragment, along with the ancillary ligand, seemed crucial for the bioactivity in A549 lung carcinoma cells versus endothelial cells. Both cell types display variable sensitivities to the complexes (IC(50)=0.6–3.5 μM). The apoptotic pathway is activated in all cases, and paraptotic cell death seems to take place at initial stages in A549 cells. Species 2–4 showed at least dual lysosomal and mitochondrial biodistribution in A549 cells, with an initial lysosomal localisation and a possible trafficking process between both organelles with time. The bimetallic Ir(III)−Au(I) complexes disrupted the mitochondrial transmembrane potential in A549 cells and increased reactive oxygen species (ROS) generation and thioredoxin reductase (TrxR) inhibition in comparison with that displayed by the monometallic complex 1. Angiogenic activity assays performed in endothelial cells revealed the promising antimetastatic potential of 1, 2 and 4. John Wiley and Sons Inc. 2021-05-29 2021-07-07 /pmc/articles/PMC8361937/ /pubmed/33860585 http://dx.doi.org/10.1002/chem.202100707 Text en © 2021 The Authors. Chemistry - A European Journal published by Wiley-VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Full Papers Redrado, Marta Benedi, Andrea Marzo, Isabel García‐Otín, Angel L. Fernández‐Moreira, Vanesa Concepción Gimeno, M. Multifunctional Heterometallic Ir(III)−Au(I) Probes as Promising Anticancer and Antiangiogenic Agents |
title | Multifunctional Heterometallic Ir(III)−Au(I) Probes as Promising Anticancer and Antiangiogenic Agents |
title_full | Multifunctional Heterometallic Ir(III)−Au(I) Probes as Promising Anticancer and Antiangiogenic Agents |
title_fullStr | Multifunctional Heterometallic Ir(III)−Au(I) Probes as Promising Anticancer and Antiangiogenic Agents |
title_full_unstemmed | Multifunctional Heterometallic Ir(III)−Au(I) Probes as Promising Anticancer and Antiangiogenic Agents |
title_short | Multifunctional Heterometallic Ir(III)−Au(I) Probes as Promising Anticancer and Antiangiogenic Agents |
title_sort | multifunctional heterometallic ir(iii)−au(i) probes as promising anticancer and antiangiogenic agents |
topic | Full Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361937/ https://www.ncbi.nlm.nih.gov/pubmed/33860585 http://dx.doi.org/10.1002/chem.202100707 |
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