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The circular RNA circ_GRHPR promotes NSCLC cell proliferation and invasion via interactions with the RNA‐binding protein PCBP2

As the most common malignancy, lung cancer is characterised by high rates of occurrence and mortality. Although circular RNAs (circRNAs) are known to act as important regulators in cancer, their role in lung cancer remains poorly understood. In this study, circ_GRHPR expression was found to be signi...

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Autores principales: Hou, Yanyan, Lin, Jiafei, Wang, Danyang, Zhang, Yingwei, Liang, Qiuli, Chen, Ning, Wu, Jiemin, Wu, Weiqi, Liu, Xiangfan, Ni, Peihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8362189/
https://www.ncbi.nlm.nih.gov/pubmed/33987874
http://dx.doi.org/10.1111/1440-1681.13523
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author Hou, Yanyan
Lin, Jiafei
Wang, Danyang
Zhang, Yingwei
Liang, Qiuli
Chen, Ning
Wu, Jiemin
Wu, Weiqi
Liu, Xiangfan
Ni, Peihua
author_facet Hou, Yanyan
Lin, Jiafei
Wang, Danyang
Zhang, Yingwei
Liang, Qiuli
Chen, Ning
Wu, Jiemin
Wu, Weiqi
Liu, Xiangfan
Ni, Peihua
author_sort Hou, Yanyan
collection PubMed
description As the most common malignancy, lung cancer is characterised by high rates of occurrence and mortality. Although circular RNAs (circRNAs) are known to act as important regulators in cancer, their role in lung cancer remains poorly understood. In this study, circ_GRHPR expression was found to be significantly upregulated in the serum of five patients with non‐small cell lung cancer (NSCLC), compared to that in healthy controls. It is expressed at high levels in NSCLC cell lines, as revealed by qRT‐PCR analysis. Functionally, we demonstrated that circ_GRHPR promotes NSCLC proliferation and invasion in vitro and in vivo by cell proliferation, transwell, cell cycle, and tumour‐forming assays. Mechanistically, RNA pull‐down and RNA immunoprecipitation assays showed that circ_GRHPR interacts with the RNA‐binding protein poly(rC)‐binding protein 2 (PCBP2) and regulates its subcellular localisation by forming the circ_GRHPR/PCBP2 complex, localizing PCBP2 mainly in the cytoplasm and reducing the proportion found in the nucleus. Furthermore, we demonstrated that four‐and‐a‐half LIM‐only protein 3 (FHL3) is a tumour‐stimulating factor in NSCLC that interacts with and is influenced by PCBP2. Circ_GRHPR increased FHL3 expression in the nucleus of NSCLC cells by decreasing PCBP2 expression therein and promoting the proliferation and invasion of NSCLC cells. Therefore, our study identified that circ_GRHPR promotes NSCLC proliferation and invasion, providing a possible explanation for its mechanism of action.
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spelling pubmed-83621892021-08-17 The circular RNA circ_GRHPR promotes NSCLC cell proliferation and invasion via interactions with the RNA‐binding protein PCBP2 Hou, Yanyan Lin, Jiafei Wang, Danyang Zhang, Yingwei Liang, Qiuli Chen, Ning Wu, Jiemin Wu, Weiqi Liu, Xiangfan Ni, Peihua Clin Exp Pharmacol Physiol Original Articles As the most common malignancy, lung cancer is characterised by high rates of occurrence and mortality. Although circular RNAs (circRNAs) are known to act as important regulators in cancer, their role in lung cancer remains poorly understood. In this study, circ_GRHPR expression was found to be significantly upregulated in the serum of five patients with non‐small cell lung cancer (NSCLC), compared to that in healthy controls. It is expressed at high levels in NSCLC cell lines, as revealed by qRT‐PCR analysis. Functionally, we demonstrated that circ_GRHPR promotes NSCLC proliferation and invasion in vitro and in vivo by cell proliferation, transwell, cell cycle, and tumour‐forming assays. Mechanistically, RNA pull‐down and RNA immunoprecipitation assays showed that circ_GRHPR interacts with the RNA‐binding protein poly(rC)‐binding protein 2 (PCBP2) and regulates its subcellular localisation by forming the circ_GRHPR/PCBP2 complex, localizing PCBP2 mainly in the cytoplasm and reducing the proportion found in the nucleus. Furthermore, we demonstrated that four‐and‐a‐half LIM‐only protein 3 (FHL3) is a tumour‐stimulating factor in NSCLC that interacts with and is influenced by PCBP2. Circ_GRHPR increased FHL3 expression in the nucleus of NSCLC cells by decreasing PCBP2 expression therein and promoting the proliferation and invasion of NSCLC cells. Therefore, our study identified that circ_GRHPR promotes NSCLC proliferation and invasion, providing a possible explanation for its mechanism of action. John Wiley and Sons Inc. 2021-05-29 2021-08 /pmc/articles/PMC8362189/ /pubmed/33987874 http://dx.doi.org/10.1111/1440-1681.13523 Text en © 2021 The Authors. Clinical and Experimental Pharmacology and Physiology published by John Wiley & Sons Australia, Ltd. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Hou, Yanyan
Lin, Jiafei
Wang, Danyang
Zhang, Yingwei
Liang, Qiuli
Chen, Ning
Wu, Jiemin
Wu, Weiqi
Liu, Xiangfan
Ni, Peihua
The circular RNA circ_GRHPR promotes NSCLC cell proliferation and invasion via interactions with the RNA‐binding protein PCBP2
title The circular RNA circ_GRHPR promotes NSCLC cell proliferation and invasion via interactions with the RNA‐binding protein PCBP2
title_full The circular RNA circ_GRHPR promotes NSCLC cell proliferation and invasion via interactions with the RNA‐binding protein PCBP2
title_fullStr The circular RNA circ_GRHPR promotes NSCLC cell proliferation and invasion via interactions with the RNA‐binding protein PCBP2
title_full_unstemmed The circular RNA circ_GRHPR promotes NSCLC cell proliferation and invasion via interactions with the RNA‐binding protein PCBP2
title_short The circular RNA circ_GRHPR promotes NSCLC cell proliferation and invasion via interactions with the RNA‐binding protein PCBP2
title_sort circular rna circ_grhpr promotes nsclc cell proliferation and invasion via interactions with the rna‐binding protein pcbp2
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8362189/
https://www.ncbi.nlm.nih.gov/pubmed/33987874
http://dx.doi.org/10.1111/1440-1681.13523
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