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Relationship between CCR5(+)FoxP3(+) Treg cells and forced expiratory volume in 1 s, peak expiratory flow in patients with severe asthma
INTRODUCTION: Severe asthma is a special clinical problem. CD4(+)CD25(high)CD127(low)Foxp3(+) Tregs play a role in maintaining appropriate immunological response. It is a known fact that Treg cells with CCR5 expression represent strong suppressive activity. It has been shown that a low number or alt...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Termedia Publishing House
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8362744/ https://www.ncbi.nlm.nih.gov/pubmed/34408594 http://dx.doi.org/10.5114/ada.2021.106202 |
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author | Kraszula, Łukasz Eusebio, Makandjou-Ola Kuna, Piotr Pietruczuk, Mirosława |
author_facet | Kraszula, Łukasz Eusebio, Makandjou-Ola Kuna, Piotr Pietruczuk, Mirosława |
author_sort | Kraszula, Łukasz |
collection | PubMed |
description | INTRODUCTION: Severe asthma is a special clinical problem. CD4(+)CD25(high)CD127(low)Foxp3(+) Tregs play a role in maintaining appropriate immunological response. It is a known fact that Treg cells with CCR5 expression represent strong suppressive activity. It has been shown that a low number or altered function of FoxP3(+) Tregs is associated with the inflammatory process and airway obstruction in asthma. AIM: To evaluate whether CCR5 Tregs expression and surface density on FoxP3(+) Treg cells depend on the severity of asthma. MATERIAL AND METHODS: The study included 50 patients with asthma (25 with severe and 25 with mild-to-moderate asthma). The control group comprised 25 healthy volunteers. The phenotype of CD4(+)CD25(high)CD127(low)Foxp3(+)CCR5(+) cells was evaluated by multicolour flow cytometry. The degree of airflow obstruction was assessed by spirometry as forced expiratory volume in 1 s (FEV(1)) and peak expiratory flow (PEF). RESULTS: The absolute count of FoxP3(+) Treg cells in patients with severe asthma was significantly decreased in comparison with the control group. MFI (median fluorescence intensity) of CCR5 expression on FoxP3(+) Treg cells was significantly decreased in severe asthma compared to the mild-to-moderate asthma and control groups. CCR5 expression on FoxP3(+) Treg cells as MFI positively correlated with lung function parameters FEV(1)% and PEF% in patients with severe asthma. CONCLUSIONS: High CCR5 Tregs expression as MFI is associated with improved in lung function parameters: FEV(1)% and PEF% in patients with severe asthma. The measurement of CCR5 expression on the surface of peripheral blood FoxP3(+) Treg cells as MFI could be an additional tool to estimate the severity of asthma. |
format | Online Article Text |
id | pubmed-8362744 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Termedia Publishing House |
record_format | MEDLINE/PubMed |
spelling | pubmed-83627442021-08-17 Relationship between CCR5(+)FoxP3(+) Treg cells and forced expiratory volume in 1 s, peak expiratory flow in patients with severe asthma Kraszula, Łukasz Eusebio, Makandjou-Ola Kuna, Piotr Pietruczuk, Mirosława Postepy Dermatol Alergol Original Paper INTRODUCTION: Severe asthma is a special clinical problem. CD4(+)CD25(high)CD127(low)Foxp3(+) Tregs play a role in maintaining appropriate immunological response. It is a known fact that Treg cells with CCR5 expression represent strong suppressive activity. It has been shown that a low number or altered function of FoxP3(+) Tregs is associated with the inflammatory process and airway obstruction in asthma. AIM: To evaluate whether CCR5 Tregs expression and surface density on FoxP3(+) Treg cells depend on the severity of asthma. MATERIAL AND METHODS: The study included 50 patients with asthma (25 with severe and 25 with mild-to-moderate asthma). The control group comprised 25 healthy volunteers. The phenotype of CD4(+)CD25(high)CD127(low)Foxp3(+)CCR5(+) cells was evaluated by multicolour flow cytometry. The degree of airflow obstruction was assessed by spirometry as forced expiratory volume in 1 s (FEV(1)) and peak expiratory flow (PEF). RESULTS: The absolute count of FoxP3(+) Treg cells in patients with severe asthma was significantly decreased in comparison with the control group. MFI (median fluorescence intensity) of CCR5 expression on FoxP3(+) Treg cells was significantly decreased in severe asthma compared to the mild-to-moderate asthma and control groups. CCR5 expression on FoxP3(+) Treg cells as MFI positively correlated with lung function parameters FEV(1)% and PEF% in patients with severe asthma. CONCLUSIONS: High CCR5 Tregs expression as MFI is associated with improved in lung function parameters: FEV(1)% and PEF% in patients with severe asthma. The measurement of CCR5 expression on the surface of peripheral blood FoxP3(+) Treg cells as MFI could be an additional tool to estimate the severity of asthma. Termedia Publishing House 2021-05-22 2021-04 /pmc/articles/PMC8362744/ /pubmed/34408594 http://dx.doi.org/10.5114/ada.2021.106202 Text en Copyright © 2021 Termedia https://creativecommons.org/licenses/by-nc-sa/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0). License (http://creativecommons.org/licenses/by-nc-sa/4.0/ (https://creativecommons.org/licenses/by-nc-sa/4.0/) ) |
spellingShingle | Original Paper Kraszula, Łukasz Eusebio, Makandjou-Ola Kuna, Piotr Pietruczuk, Mirosława Relationship between CCR5(+)FoxP3(+) Treg cells and forced expiratory volume in 1 s, peak expiratory flow in patients with severe asthma |
title | Relationship between CCR5(+)FoxP3(+) Treg cells and forced expiratory volume in 1 s, peak expiratory flow in patients with severe asthma |
title_full | Relationship between CCR5(+)FoxP3(+) Treg cells and forced expiratory volume in 1 s, peak expiratory flow in patients with severe asthma |
title_fullStr | Relationship between CCR5(+)FoxP3(+) Treg cells and forced expiratory volume in 1 s, peak expiratory flow in patients with severe asthma |
title_full_unstemmed | Relationship between CCR5(+)FoxP3(+) Treg cells and forced expiratory volume in 1 s, peak expiratory flow in patients with severe asthma |
title_short | Relationship between CCR5(+)FoxP3(+) Treg cells and forced expiratory volume in 1 s, peak expiratory flow in patients with severe asthma |
title_sort | relationship between ccr5(+)foxp3(+) treg cells and forced expiratory volume in 1 s, peak expiratory flow in patients with severe asthma |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8362744/ https://www.ncbi.nlm.nih.gov/pubmed/34408594 http://dx.doi.org/10.5114/ada.2021.106202 |
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