Cargando…
Simultaneous Detection of Ebola Virus and Pathogens Associated With Hemorrhagic Fever by an Oligonucleotide Microarray
Ebola virus infection causes severe hemorrhagic fever, and its mortality rates varied from 25 to 90% in the previous outbreaks. The highly infectious and lethal nature of this virus highlights the need for reliable and sensitive diagnostic methods to distinguish it from other diseases present with s...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363200/ https://www.ncbi.nlm.nih.gov/pubmed/34394063 http://dx.doi.org/10.3389/fmicb.2021.713372 |
_version_ | 1783738307599925248 |
---|---|
author | Yao, Wenwu Yang, Zhangnv Lou, Xiuyu Mao, Haiyan Yan, Hao Zhang, Yanjun |
author_facet | Yao, Wenwu Yang, Zhangnv Lou, Xiuyu Mao, Haiyan Yan, Hao Zhang, Yanjun |
author_sort | Yao, Wenwu |
collection | PubMed |
description | Ebola virus infection causes severe hemorrhagic fever, and its mortality rates varied from 25 to 90% in the previous outbreaks. The highly infectious and lethal nature of this virus highlights the need for reliable and sensitive diagnostic methods to distinguish it from other diseases present with similar clinical symptoms. Based on multiplex polymerase chain reaction (PCR) and oligonucleotide microarray technology, a cost-effective, multipathogen and high-throughput method was developed for simultaneous detection of Ebola virus and other pathogens associated with hemorrhagic fever, including Marburg virus, Lassa fever virus, Junin virus, Machupo virus, Rift Valley fever virus, Crimean-Congo hemorrhagic fever virus, malaria parasite, hantavirus, severe fever with thrombocytopenia syndrome virus, dengue virus, yellow fever virus, Chikungunya virus, influenza A virus, and influenza B virus. This assay had an excellent specificity for target pathogens, without overlap signal between the probes. The limit of detection was approximately 10(3) pathogen copies/μl. A total of 60 positive nucleic acid samples for different pathogens were detected, a concordance of 100% was observed between microarray assay and real-time PCR analysis. Consequently, the described oligonucleotide microarray may be specific and sensitive assay for diagnosis and surveillance of infections caused by Ebola virus and other species of hemorrhagic fever pathogens. |
format | Online Article Text |
id | pubmed-8363200 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83632002021-08-14 Simultaneous Detection of Ebola Virus and Pathogens Associated With Hemorrhagic Fever by an Oligonucleotide Microarray Yao, Wenwu Yang, Zhangnv Lou, Xiuyu Mao, Haiyan Yan, Hao Zhang, Yanjun Front Microbiol Microbiology Ebola virus infection causes severe hemorrhagic fever, and its mortality rates varied from 25 to 90% in the previous outbreaks. The highly infectious and lethal nature of this virus highlights the need for reliable and sensitive diagnostic methods to distinguish it from other diseases present with similar clinical symptoms. Based on multiplex polymerase chain reaction (PCR) and oligonucleotide microarray technology, a cost-effective, multipathogen and high-throughput method was developed for simultaneous detection of Ebola virus and other pathogens associated with hemorrhagic fever, including Marburg virus, Lassa fever virus, Junin virus, Machupo virus, Rift Valley fever virus, Crimean-Congo hemorrhagic fever virus, malaria parasite, hantavirus, severe fever with thrombocytopenia syndrome virus, dengue virus, yellow fever virus, Chikungunya virus, influenza A virus, and influenza B virus. This assay had an excellent specificity for target pathogens, without overlap signal between the probes. The limit of detection was approximately 10(3) pathogen copies/μl. A total of 60 positive nucleic acid samples for different pathogens were detected, a concordance of 100% was observed between microarray assay and real-time PCR analysis. Consequently, the described oligonucleotide microarray may be specific and sensitive assay for diagnosis and surveillance of infections caused by Ebola virus and other species of hemorrhagic fever pathogens. Frontiers Media S.A. 2021-07-30 /pmc/articles/PMC8363200/ /pubmed/34394063 http://dx.doi.org/10.3389/fmicb.2021.713372 Text en Copyright © 2021 Yao, Yang, Lou, Mao, Yan and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Yao, Wenwu Yang, Zhangnv Lou, Xiuyu Mao, Haiyan Yan, Hao Zhang, Yanjun Simultaneous Detection of Ebola Virus and Pathogens Associated With Hemorrhagic Fever by an Oligonucleotide Microarray |
title | Simultaneous Detection of Ebola Virus and Pathogens Associated With Hemorrhagic Fever by an Oligonucleotide Microarray |
title_full | Simultaneous Detection of Ebola Virus and Pathogens Associated With Hemorrhagic Fever by an Oligonucleotide Microarray |
title_fullStr | Simultaneous Detection of Ebola Virus and Pathogens Associated With Hemorrhagic Fever by an Oligonucleotide Microarray |
title_full_unstemmed | Simultaneous Detection of Ebola Virus and Pathogens Associated With Hemorrhagic Fever by an Oligonucleotide Microarray |
title_short | Simultaneous Detection of Ebola Virus and Pathogens Associated With Hemorrhagic Fever by an Oligonucleotide Microarray |
title_sort | simultaneous detection of ebola virus and pathogens associated with hemorrhagic fever by an oligonucleotide microarray |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363200/ https://www.ncbi.nlm.nih.gov/pubmed/34394063 http://dx.doi.org/10.3389/fmicb.2021.713372 |
work_keys_str_mv | AT yaowenwu simultaneousdetectionofebolavirusandpathogensassociatedwithhemorrhagicfeverbyanoligonucleotidemicroarray AT yangzhangnv simultaneousdetectionofebolavirusandpathogensassociatedwithhemorrhagicfeverbyanoligonucleotidemicroarray AT louxiuyu simultaneousdetectionofebolavirusandpathogensassociatedwithhemorrhagicfeverbyanoligonucleotidemicroarray AT maohaiyan simultaneousdetectionofebolavirusandpathogensassociatedwithhemorrhagicfeverbyanoligonucleotidemicroarray AT yanhao simultaneousdetectionofebolavirusandpathogensassociatedwithhemorrhagicfeverbyanoligonucleotidemicroarray AT zhangyanjun simultaneousdetectionofebolavirusandpathogensassociatedwithhemorrhagicfeverbyanoligonucleotidemicroarray |