Cargando…
Lactobacillus plantarum in Dual-Species Biofilms With Listeria monocytogenes Enhanced the Anti-Listeria Activity of a Commercial Disinfectant Based on Hydrogen Peroxide and Peracetic Acid
The aim of this work was to investigate the effect of dual-species biofilms of Listeria monocytogenes with Lactobacillus plantarum on the anti-Listeria activity of a hydrogen peroxide/peracetic acid based commercial disinfectant (P3, Oxonia) when using conditions approaching the food industry enviro...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363201/ https://www.ncbi.nlm.nih.gov/pubmed/34394015 http://dx.doi.org/10.3389/fmicb.2021.631627 |
_version_ | 1783738307842146304 |
---|---|
author | Bonneville, Lourenço Maia, Vera Barroso, Inês Martínez-Suárez, Joaquín V. Brito, Luisa |
author_facet | Bonneville, Lourenço Maia, Vera Barroso, Inês Martínez-Suárez, Joaquín V. Brito, Luisa |
author_sort | Bonneville, Lourenço |
collection | PubMed |
description | The aim of this work was to investigate the effect of dual-species biofilms of Listeria monocytogenes with Lactobacillus plantarum on the anti-Listeria activity of a hydrogen peroxide/peracetic acid based commercial disinfectant (P3, Oxonia) when using conditions approaching the food industry environment. Nine strains of L. monocytogenes, including eight persistent strains collected from the meat industry and one laboratory control strain, were used in mono and in dual-species biofilms with a strain of L. plantarum. Biofilms were produced on stainless steel coupons (SSCs), at 11°C (low temperature) or at 25°C (control temperature), in TSB-YE (control rich medium) or in 1/10 diluted TSB-YE (mimicking the situation of biofilm formation after a deficient industrial cleaning procedure). The biofilm forming ability of the strains was evaluated by enumeration of viable cells, and the antibiofilm activity of P3 was assessed by the log reduction of viable cells on SSC. In both nutrient conditions and at low temperature, there was no significant difference (p > 0.05) between L. monocytogenes biofilm forming ability in mono- and in dual-species biofilms. In dual-species biofilms, L. monocytogenes was the dominant species. However, it was generally more susceptible to the lower concentration of P3 0.5% (v/v) than in pure culture biofilms. The presence of L. plantarum, although without significant interference in the number of viable cells of L. monocytogenes, enhanced the efficacy of the anti-Listeria activity of P3, since dual-species biofilms were easier to control. The results presented here reinforce the importance of the investigation into co-culture biofilms produced in food industry conditions, namely at low temperatures, when susceptibility to sanitizers is being assessed. |
format | Online Article Text |
id | pubmed-8363201 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83632012021-08-14 Lactobacillus plantarum in Dual-Species Biofilms With Listeria monocytogenes Enhanced the Anti-Listeria Activity of a Commercial Disinfectant Based on Hydrogen Peroxide and Peracetic Acid Bonneville, Lourenço Maia, Vera Barroso, Inês Martínez-Suárez, Joaquín V. Brito, Luisa Front Microbiol Microbiology The aim of this work was to investigate the effect of dual-species biofilms of Listeria monocytogenes with Lactobacillus plantarum on the anti-Listeria activity of a hydrogen peroxide/peracetic acid based commercial disinfectant (P3, Oxonia) when using conditions approaching the food industry environment. Nine strains of L. monocytogenes, including eight persistent strains collected from the meat industry and one laboratory control strain, were used in mono and in dual-species biofilms with a strain of L. plantarum. Biofilms were produced on stainless steel coupons (SSCs), at 11°C (low temperature) or at 25°C (control temperature), in TSB-YE (control rich medium) or in 1/10 diluted TSB-YE (mimicking the situation of biofilm formation after a deficient industrial cleaning procedure). The biofilm forming ability of the strains was evaluated by enumeration of viable cells, and the antibiofilm activity of P3 was assessed by the log reduction of viable cells on SSC. In both nutrient conditions and at low temperature, there was no significant difference (p > 0.05) between L. monocytogenes biofilm forming ability in mono- and in dual-species biofilms. In dual-species biofilms, L. monocytogenes was the dominant species. However, it was generally more susceptible to the lower concentration of P3 0.5% (v/v) than in pure culture biofilms. The presence of L. plantarum, although without significant interference in the number of viable cells of L. monocytogenes, enhanced the efficacy of the anti-Listeria activity of P3, since dual-species biofilms were easier to control. The results presented here reinforce the importance of the investigation into co-culture biofilms produced in food industry conditions, namely at low temperatures, when susceptibility to sanitizers is being assessed. Frontiers Media S.A. 2021-07-30 /pmc/articles/PMC8363201/ /pubmed/34394015 http://dx.doi.org/10.3389/fmicb.2021.631627 Text en Copyright © 2021 Bonneville, Maia, Barroso, Martínez-Suárez and Brito. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Bonneville, Lourenço Maia, Vera Barroso, Inês Martínez-Suárez, Joaquín V. Brito, Luisa Lactobacillus plantarum in Dual-Species Biofilms With Listeria monocytogenes Enhanced the Anti-Listeria Activity of a Commercial Disinfectant Based on Hydrogen Peroxide and Peracetic Acid |
title | Lactobacillus plantarum in Dual-Species Biofilms With Listeria monocytogenes Enhanced the Anti-Listeria Activity of a Commercial Disinfectant Based on Hydrogen Peroxide and Peracetic Acid |
title_full | Lactobacillus plantarum in Dual-Species Biofilms With Listeria monocytogenes Enhanced the Anti-Listeria Activity of a Commercial Disinfectant Based on Hydrogen Peroxide and Peracetic Acid |
title_fullStr | Lactobacillus plantarum in Dual-Species Biofilms With Listeria monocytogenes Enhanced the Anti-Listeria Activity of a Commercial Disinfectant Based on Hydrogen Peroxide and Peracetic Acid |
title_full_unstemmed | Lactobacillus plantarum in Dual-Species Biofilms With Listeria monocytogenes Enhanced the Anti-Listeria Activity of a Commercial Disinfectant Based on Hydrogen Peroxide and Peracetic Acid |
title_short | Lactobacillus plantarum in Dual-Species Biofilms With Listeria monocytogenes Enhanced the Anti-Listeria Activity of a Commercial Disinfectant Based on Hydrogen Peroxide and Peracetic Acid |
title_sort | lactobacillus plantarum in dual-species biofilms with listeria monocytogenes enhanced the anti-listeria activity of a commercial disinfectant based on hydrogen peroxide and peracetic acid |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363201/ https://www.ncbi.nlm.nih.gov/pubmed/34394015 http://dx.doi.org/10.3389/fmicb.2021.631627 |
work_keys_str_mv | AT bonnevillelourenco lactobacillusplantarumindualspeciesbiofilmswithlisteriamonocytogenesenhancedtheantilisteriaactivityofacommercialdisinfectantbasedonhydrogenperoxideandperaceticacid AT maiavera lactobacillusplantarumindualspeciesbiofilmswithlisteriamonocytogenesenhancedtheantilisteriaactivityofacommercialdisinfectantbasedonhydrogenperoxideandperaceticacid AT barrosoines lactobacillusplantarumindualspeciesbiofilmswithlisteriamonocytogenesenhancedtheantilisteriaactivityofacommercialdisinfectantbasedonhydrogenperoxideandperaceticacid AT martinezsuarezjoaquinv lactobacillusplantarumindualspeciesbiofilmswithlisteriamonocytogenesenhancedtheantilisteriaactivityofacommercialdisinfectantbasedonhydrogenperoxideandperaceticacid AT britoluisa lactobacillusplantarumindualspeciesbiofilmswithlisteriamonocytogenesenhancedtheantilisteriaactivityofacommercialdisinfectantbasedonhydrogenperoxideandperaceticacid |