Cargando…
Sustained expression of unc-4 homeobox gene and unc-37/Groucho in postmitotic neurons specifies the spatial organization of the cholinergic synapses in C. elegans
Neuronal cell fate determinants establish the identities of neurons by controlling gene expression to regulate neuronal morphology and synaptic connectivity. However, it is not understood if neuronal cell fate determinants have postmitotic functions in synapse pattern formation. Here we identify a n...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363302/ https://www.ncbi.nlm.nih.gov/pubmed/34388088 http://dx.doi.org/10.7554/eLife.66011 |
_version_ | 1783738322989875200 |
---|---|
author | Kurashina, Mizuki Wang, Jane Lin, Jeffrey Lee, Kathy Kyungeun Johal, Arpun Mizumoto, Kota |
author_facet | Kurashina, Mizuki Wang, Jane Lin, Jeffrey Lee, Kathy Kyungeun Johal, Arpun Mizumoto, Kota |
author_sort | Kurashina, Mizuki |
collection | PubMed |
description | Neuronal cell fate determinants establish the identities of neurons by controlling gene expression to regulate neuronal morphology and synaptic connectivity. However, it is not understood if neuronal cell fate determinants have postmitotic functions in synapse pattern formation. Here we identify a novel role for UNC-4 homeobox protein and its corepressor UNC-37/Groucho, in tiled synaptic patterning of the cholinergic motor neurons in Caenorhabditis elegans. We show that unc-4 is not required during neurogenesis but is required in the postmitotic neurons for proper synapse patterning. In contrast, unc-37 is required in both developing and postmitotic neurons. The synaptic tiling defects of unc-4 mutants are suppressed by bar-1/β-catenin mutation, which positively regulates the expression of ceh-12/HB9. Ectopic ceh-12 expression partly underlies the synaptic tiling defects of unc-4 and unc-37 mutants. Our results reveal a novel postmitotic role of neuronal cell fate determinants in synapse pattern formation through inhibiting the canonical Wnt signaling pathway. |
format | Online Article Text |
id | pubmed-8363302 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-83633022021-08-16 Sustained expression of unc-4 homeobox gene and unc-37/Groucho in postmitotic neurons specifies the spatial organization of the cholinergic synapses in C. elegans Kurashina, Mizuki Wang, Jane Lin, Jeffrey Lee, Kathy Kyungeun Johal, Arpun Mizumoto, Kota eLife Developmental Biology Neuronal cell fate determinants establish the identities of neurons by controlling gene expression to regulate neuronal morphology and synaptic connectivity. However, it is not understood if neuronal cell fate determinants have postmitotic functions in synapse pattern formation. Here we identify a novel role for UNC-4 homeobox protein and its corepressor UNC-37/Groucho, in tiled synaptic patterning of the cholinergic motor neurons in Caenorhabditis elegans. We show that unc-4 is not required during neurogenesis but is required in the postmitotic neurons for proper synapse patterning. In contrast, unc-37 is required in both developing and postmitotic neurons. The synaptic tiling defects of unc-4 mutants are suppressed by bar-1/β-catenin mutation, which positively regulates the expression of ceh-12/HB9. Ectopic ceh-12 expression partly underlies the synaptic tiling defects of unc-4 and unc-37 mutants. Our results reveal a novel postmitotic role of neuronal cell fate determinants in synapse pattern formation through inhibiting the canonical Wnt signaling pathway. eLife Sciences Publications, Ltd 2021-08-13 /pmc/articles/PMC8363302/ /pubmed/34388088 http://dx.doi.org/10.7554/eLife.66011 Text en © 2021, Kurashina et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Developmental Biology Kurashina, Mizuki Wang, Jane Lin, Jeffrey Lee, Kathy Kyungeun Johal, Arpun Mizumoto, Kota Sustained expression of unc-4 homeobox gene and unc-37/Groucho in postmitotic neurons specifies the spatial organization of the cholinergic synapses in C. elegans |
title | Sustained expression of unc-4 homeobox gene and unc-37/Groucho in postmitotic neurons specifies the spatial organization of the cholinergic synapses in C. elegans |
title_full | Sustained expression of unc-4 homeobox gene and unc-37/Groucho in postmitotic neurons specifies the spatial organization of the cholinergic synapses in C. elegans |
title_fullStr | Sustained expression of unc-4 homeobox gene and unc-37/Groucho in postmitotic neurons specifies the spatial organization of the cholinergic synapses in C. elegans |
title_full_unstemmed | Sustained expression of unc-4 homeobox gene and unc-37/Groucho in postmitotic neurons specifies the spatial organization of the cholinergic synapses in C. elegans |
title_short | Sustained expression of unc-4 homeobox gene and unc-37/Groucho in postmitotic neurons specifies the spatial organization of the cholinergic synapses in C. elegans |
title_sort | sustained expression of unc-4 homeobox gene and unc-37/groucho in postmitotic neurons specifies the spatial organization of the cholinergic synapses in c. elegans |
topic | Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363302/ https://www.ncbi.nlm.nih.gov/pubmed/34388088 http://dx.doi.org/10.7554/eLife.66011 |
work_keys_str_mv | AT kurashinamizuki sustainedexpressionofunc4homeoboxgeneandunc37grouchoinpostmitoticneuronsspecifiesthespatialorganizationofthecholinergicsynapsesincelegans AT wangjane sustainedexpressionofunc4homeoboxgeneandunc37grouchoinpostmitoticneuronsspecifiesthespatialorganizationofthecholinergicsynapsesincelegans AT linjeffrey sustainedexpressionofunc4homeoboxgeneandunc37grouchoinpostmitoticneuronsspecifiesthespatialorganizationofthecholinergicsynapsesincelegans AT leekathykyungeun sustainedexpressionofunc4homeoboxgeneandunc37grouchoinpostmitoticneuronsspecifiesthespatialorganizationofthecholinergicsynapsesincelegans AT johalarpun sustainedexpressionofunc4homeoboxgeneandunc37grouchoinpostmitoticneuronsspecifiesthespatialorganizationofthecholinergicsynapsesincelegans AT mizumotokota sustainedexpressionofunc4homeoboxgeneandunc37grouchoinpostmitoticneuronsspecifiesthespatialorganizationofthecholinergicsynapsesincelegans |