Cargando…
In situ imaging reveals disparity between prostaglandin localization and abundance of prostaglandin synthases
Prostaglandins are important lipids involved in mediating many physiological processes, such as allergic responses, inflammation, and pregnancy. However, technical limitations of in-situ prostaglandin detection in tissue have led researchers to infer prostaglandin tissue distributions from localizat...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363604/ https://www.ncbi.nlm.nih.gov/pubmed/34389796 http://dx.doi.org/10.1038/s42003-021-02488-1 |
_version_ | 1783738378254024704 |
---|---|
author | Duncan, Kyle D. Sun, Xiaofei Baker, Erin S. Dey, Sudhansu K. Lanekoff, Ingela |
author_facet | Duncan, Kyle D. Sun, Xiaofei Baker, Erin S. Dey, Sudhansu K. Lanekoff, Ingela |
author_sort | Duncan, Kyle D. |
collection | PubMed |
description | Prostaglandins are important lipids involved in mediating many physiological processes, such as allergic responses, inflammation, and pregnancy. However, technical limitations of in-situ prostaglandin detection in tissue have led researchers to infer prostaglandin tissue distributions from localization of regulatory synthases, such as COX1 and COX2. Herein, we apply a novel mass spectrometry imaging method for direct in situ tissue localization of prostaglandins, and combine it with techniques for protein expression and RNA localization. We report that prostaglandin D(2), its precursors, and downstream synthases co-localize with the highest expression of COX1, and not COX2. Further, we study tissue with a conditional deletion of transformation-related protein 53 where pregnancy success is low and confirm that PG levels are altered, although localization is conserved. Our studies reveal that the abundance of COX and prostaglandin D(2) synthases in cellular regions does not mirror the regional abundance of prostaglandins. Thus, we deduce that prostaglandins tissue localization and abundance may not be inferred by COX or prostaglandin synthases in uterine tissue, and must be resolved by an in situ prostaglandin imaging. |
format | Online Article Text |
id | pubmed-8363604 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-83636042021-08-19 In situ imaging reveals disparity between prostaglandin localization and abundance of prostaglandin synthases Duncan, Kyle D. Sun, Xiaofei Baker, Erin S. Dey, Sudhansu K. Lanekoff, Ingela Commun Biol Article Prostaglandins are important lipids involved in mediating many physiological processes, such as allergic responses, inflammation, and pregnancy. However, technical limitations of in-situ prostaglandin detection in tissue have led researchers to infer prostaglandin tissue distributions from localization of regulatory synthases, such as COX1 and COX2. Herein, we apply a novel mass spectrometry imaging method for direct in situ tissue localization of prostaglandins, and combine it with techniques for protein expression and RNA localization. We report that prostaglandin D(2), its precursors, and downstream synthases co-localize with the highest expression of COX1, and not COX2. Further, we study tissue with a conditional deletion of transformation-related protein 53 where pregnancy success is low and confirm that PG levels are altered, although localization is conserved. Our studies reveal that the abundance of COX and prostaglandin D(2) synthases in cellular regions does not mirror the regional abundance of prostaglandins. Thus, we deduce that prostaglandins tissue localization and abundance may not be inferred by COX or prostaglandin synthases in uterine tissue, and must be resolved by an in situ prostaglandin imaging. Nature Publishing Group UK 2021-08-13 /pmc/articles/PMC8363604/ /pubmed/34389796 http://dx.doi.org/10.1038/s42003-021-02488-1 Text en © The Author(s) 2021, corrected publication 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Duncan, Kyle D. Sun, Xiaofei Baker, Erin S. Dey, Sudhansu K. Lanekoff, Ingela In situ imaging reveals disparity between prostaglandin localization and abundance of prostaglandin synthases |
title | In situ imaging reveals disparity between prostaglandin localization and abundance of prostaglandin synthases |
title_full | In situ imaging reveals disparity between prostaglandin localization and abundance of prostaglandin synthases |
title_fullStr | In situ imaging reveals disparity between prostaglandin localization and abundance of prostaglandin synthases |
title_full_unstemmed | In situ imaging reveals disparity between prostaglandin localization and abundance of prostaglandin synthases |
title_short | In situ imaging reveals disparity between prostaglandin localization and abundance of prostaglandin synthases |
title_sort | in situ imaging reveals disparity between prostaglandin localization and abundance of prostaglandin synthases |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363604/ https://www.ncbi.nlm.nih.gov/pubmed/34389796 http://dx.doi.org/10.1038/s42003-021-02488-1 |
work_keys_str_mv | AT duncankyled insituimagingrevealsdisparitybetweenprostaglandinlocalizationandabundanceofprostaglandinsynthases AT sunxiaofei insituimagingrevealsdisparitybetweenprostaglandinlocalizationandabundanceofprostaglandinsynthases AT bakererins insituimagingrevealsdisparitybetweenprostaglandinlocalizationandabundanceofprostaglandinsynthases AT deysudhansuk insituimagingrevealsdisparitybetweenprostaglandinlocalizationandabundanceofprostaglandinsynthases AT lanekoffingela insituimagingrevealsdisparitybetweenprostaglandinlocalizationandabundanceofprostaglandinsynthases |