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In situ imaging reveals disparity between prostaglandin localization and abundance of prostaglandin synthases

Prostaglandins are important lipids involved in mediating many physiological processes, such as allergic responses, inflammation, and pregnancy. However, technical limitations of in-situ prostaglandin detection in tissue have led researchers to infer prostaglandin tissue distributions from localizat...

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Autores principales: Duncan, Kyle D., Sun, Xiaofei, Baker, Erin S., Dey, Sudhansu K., Lanekoff, Ingela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363604/
https://www.ncbi.nlm.nih.gov/pubmed/34389796
http://dx.doi.org/10.1038/s42003-021-02488-1
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author Duncan, Kyle D.
Sun, Xiaofei
Baker, Erin S.
Dey, Sudhansu K.
Lanekoff, Ingela
author_facet Duncan, Kyle D.
Sun, Xiaofei
Baker, Erin S.
Dey, Sudhansu K.
Lanekoff, Ingela
author_sort Duncan, Kyle D.
collection PubMed
description Prostaglandins are important lipids involved in mediating many physiological processes, such as allergic responses, inflammation, and pregnancy. However, technical limitations of in-situ prostaglandin detection in tissue have led researchers to infer prostaglandin tissue distributions from localization of regulatory synthases, such as COX1 and COX2. Herein, we apply a novel mass spectrometry imaging method for direct in situ tissue localization of prostaglandins, and combine it with techniques for protein expression and RNA localization. We report that prostaglandin D(2), its precursors, and downstream synthases co-localize with the highest expression of COX1, and not COX2. Further, we study tissue with a conditional deletion of transformation-related protein 53 where pregnancy success is low and confirm that PG levels are altered, although localization is conserved. Our studies reveal that the abundance of COX and prostaglandin D(2) synthases in cellular regions does not mirror the regional abundance of prostaglandins. Thus, we deduce that prostaglandins tissue localization and abundance may not be inferred by COX or prostaglandin synthases in uterine tissue, and must be resolved by an in situ prostaglandin imaging.
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spelling pubmed-83636042021-08-19 In situ imaging reveals disparity between prostaglandin localization and abundance of prostaglandin synthases Duncan, Kyle D. Sun, Xiaofei Baker, Erin S. Dey, Sudhansu K. Lanekoff, Ingela Commun Biol Article Prostaglandins are important lipids involved in mediating many physiological processes, such as allergic responses, inflammation, and pregnancy. However, technical limitations of in-situ prostaglandin detection in tissue have led researchers to infer prostaglandin tissue distributions from localization of regulatory synthases, such as COX1 and COX2. Herein, we apply a novel mass spectrometry imaging method for direct in situ tissue localization of prostaglandins, and combine it with techniques for protein expression and RNA localization. We report that prostaglandin D(2), its precursors, and downstream synthases co-localize with the highest expression of COX1, and not COX2. Further, we study tissue with a conditional deletion of transformation-related protein 53 where pregnancy success is low and confirm that PG levels are altered, although localization is conserved. Our studies reveal that the abundance of COX and prostaglandin D(2) synthases in cellular regions does not mirror the regional abundance of prostaglandins. Thus, we deduce that prostaglandins tissue localization and abundance may not be inferred by COX or prostaglandin synthases in uterine tissue, and must be resolved by an in situ prostaglandin imaging. Nature Publishing Group UK 2021-08-13 /pmc/articles/PMC8363604/ /pubmed/34389796 http://dx.doi.org/10.1038/s42003-021-02488-1 Text en © The Author(s) 2021, corrected publication 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Duncan, Kyle D.
Sun, Xiaofei
Baker, Erin S.
Dey, Sudhansu K.
Lanekoff, Ingela
In situ imaging reveals disparity between prostaglandin localization and abundance of prostaglandin synthases
title In situ imaging reveals disparity between prostaglandin localization and abundance of prostaglandin synthases
title_full In situ imaging reveals disparity between prostaglandin localization and abundance of prostaglandin synthases
title_fullStr In situ imaging reveals disparity between prostaglandin localization and abundance of prostaglandin synthases
title_full_unstemmed In situ imaging reveals disparity between prostaglandin localization and abundance of prostaglandin synthases
title_short In situ imaging reveals disparity between prostaglandin localization and abundance of prostaglandin synthases
title_sort in situ imaging reveals disparity between prostaglandin localization and abundance of prostaglandin synthases
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363604/
https://www.ncbi.nlm.nih.gov/pubmed/34389796
http://dx.doi.org/10.1038/s42003-021-02488-1
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