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Robust sequential biophysical fractionation of blood plasma to study variations in the biomolecular landscape of systemically circulating extracellular vesicles across clinical conditions
Separating extracellular vesicles (EV) from blood plasma is challenging and complicates their biological understanding and biomarker development. In this study, we fractionate blood plasma by combining size‐exclusion chromatography (SEC) and OptiPrep density gradient centrifugation to study clinical...
| Autores principales: | , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363909/ https://www.ncbi.nlm.nih.gov/pubmed/34429857 http://dx.doi.org/10.1002/jev2.12122 |
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| author | Vergauwen, Glenn Tulkens, Joeri Pinheiro, Cláudio Avila Cobos, Francisco Dedeyne, Sándor De Scheerder, Marie‐Angélique Vandekerckhove, Linos Impens, Francis Miinalainen, Ilkka Braems, Geert Gevaert, Kris Mestdagh, Pieter Vandesompele, Jo Denys, Hannelore De Wever, Olivier Hendrix, An |
| author_facet | Vergauwen, Glenn Tulkens, Joeri Pinheiro, Cláudio Avila Cobos, Francisco Dedeyne, Sándor De Scheerder, Marie‐Angélique Vandekerckhove, Linos Impens, Francis Miinalainen, Ilkka Braems, Geert Gevaert, Kris Mestdagh, Pieter Vandesompele, Jo Denys, Hannelore De Wever, Olivier Hendrix, An |
| author_sort | Vergauwen, Glenn |
| collection | PubMed |
| description | Separating extracellular vesicles (EV) from blood plasma is challenging and complicates their biological understanding and biomarker development. In this study, we fractionate blood plasma by combining size‐exclusion chromatography (SEC) and OptiPrep density gradient centrifugation to study clinical context‐dependent and time‐dependent variations in the biomolecular landscape of systemically circulating EV. Using pooled blood plasma samples from breast cancer patients, we first demonstrate the technical repeatability of blood plasma fractionation. Using serial blood plasma samples from HIV and ovarian cancer patients (n = 10) we next show that EV carry a clinical context‐dependent and/or time‐dependent protein and small RNA composition, including miRNA and tRNA. In addition, differential analysis of blood plasma fractions provides a catalogue of putative proteins not associated with systemically circulating EV. In conclusion, the implementation of blood plasma fractionation allows to advance the biological understanding and biomarker development of systemically circulating EV. |
| format | Online Article Text |
| id | pubmed-8363909 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| publisher | John Wiley and Sons Inc. |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-83639092021-08-23 Robust sequential biophysical fractionation of blood plasma to study variations in the biomolecular landscape of systemically circulating extracellular vesicles across clinical conditions Vergauwen, Glenn Tulkens, Joeri Pinheiro, Cláudio Avila Cobos, Francisco Dedeyne, Sándor De Scheerder, Marie‐Angélique Vandekerckhove, Linos Impens, Francis Miinalainen, Ilkka Braems, Geert Gevaert, Kris Mestdagh, Pieter Vandesompele, Jo Denys, Hannelore De Wever, Olivier Hendrix, An J Extracell Vesicles Research Articles Separating extracellular vesicles (EV) from blood plasma is challenging and complicates their biological understanding and biomarker development. In this study, we fractionate blood plasma by combining size‐exclusion chromatography (SEC) and OptiPrep density gradient centrifugation to study clinical context‐dependent and time‐dependent variations in the biomolecular landscape of systemically circulating EV. Using pooled blood plasma samples from breast cancer patients, we first demonstrate the technical repeatability of blood plasma fractionation. Using serial blood plasma samples from HIV and ovarian cancer patients (n = 10) we next show that EV carry a clinical context‐dependent and/or time‐dependent protein and small RNA composition, including miRNA and tRNA. In addition, differential analysis of blood plasma fractions provides a catalogue of putative proteins not associated with systemically circulating EV. In conclusion, the implementation of blood plasma fractionation allows to advance the biological understanding and biomarker development of systemically circulating EV. John Wiley and Sons Inc. 2021-08-14 2021-08 /pmc/articles/PMC8363909/ /pubmed/34429857 http://dx.doi.org/10.1002/jev2.12122 Text en © 2021 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| spellingShingle | Research Articles Vergauwen, Glenn Tulkens, Joeri Pinheiro, Cláudio Avila Cobos, Francisco Dedeyne, Sándor De Scheerder, Marie‐Angélique Vandekerckhove, Linos Impens, Francis Miinalainen, Ilkka Braems, Geert Gevaert, Kris Mestdagh, Pieter Vandesompele, Jo Denys, Hannelore De Wever, Olivier Hendrix, An Robust sequential biophysical fractionation of blood plasma to study variations in the biomolecular landscape of systemically circulating extracellular vesicles across clinical conditions |
| title | Robust sequential biophysical fractionation of blood plasma to study variations in the biomolecular landscape of systemically circulating extracellular vesicles across clinical conditions |
| title_full | Robust sequential biophysical fractionation of blood plasma to study variations in the biomolecular landscape of systemically circulating extracellular vesicles across clinical conditions |
| title_fullStr | Robust sequential biophysical fractionation of blood plasma to study variations in the biomolecular landscape of systemically circulating extracellular vesicles across clinical conditions |
| title_full_unstemmed | Robust sequential biophysical fractionation of blood plasma to study variations in the biomolecular landscape of systemically circulating extracellular vesicles across clinical conditions |
| title_short | Robust sequential biophysical fractionation of blood plasma to study variations in the biomolecular landscape of systemically circulating extracellular vesicles across clinical conditions |
| title_sort | robust sequential biophysical fractionation of blood plasma to study variations in the biomolecular landscape of systemically circulating extracellular vesicles across clinical conditions |
| topic | Research Articles |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363909/ https://www.ncbi.nlm.nih.gov/pubmed/34429857 http://dx.doi.org/10.1002/jev2.12122 |
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