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Robust sequential biophysical fractionation of blood plasma to study variations in the biomolecular landscape of systemically circulating extracellular vesicles across clinical conditions
Separating extracellular vesicles (EV) from blood plasma is challenging and complicates their biological understanding and biomarker development. In this study, we fractionate blood plasma by combining size‐exclusion chromatography (SEC) and OptiPrep density gradient centrifugation to study clinical...
Autores principales: | Vergauwen, Glenn, Tulkens, Joeri, Pinheiro, Cláudio, Avila Cobos, Francisco, Dedeyne, Sándor, De Scheerder, Marie‐Angélique, Vandekerckhove, Linos, Impens, Francis, Miinalainen, Ilkka, Braems, Geert, Gevaert, Kris, Mestdagh, Pieter, Vandesompele, Jo, Denys, Hannelore, De Wever, Olivier, Hendrix, An |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363909/ https://www.ncbi.nlm.nih.gov/pubmed/34429857 http://dx.doi.org/10.1002/jev2.12122 |
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