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Effect of bacterial infection on sperm quality and DNA fragmentation in subfertile men with Leukocytospermia

BACKGROUND: Although bacterial infections have been recognized as a possible cause of male infertility, the effect of bacterial infections on sperm quality and sperm DNA fragmentation remains controversial. The current study aimed to investigate the prevalence rate of bacterial infection in subferti...

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Autores principales: Eini, Fatemeh, Kutenaei, Maryam Azizi, Zareei, Fayegheh, Dastjerdi, Zeinolabedin Sharifian, Shirzeyli, Maryam Hosseinzadeh, Salehi, Ensieh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364116/
https://www.ncbi.nlm.nih.gov/pubmed/34388964
http://dx.doi.org/10.1186/s12860-021-00380-8
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author Eini, Fatemeh
Kutenaei, Maryam Azizi
Zareei, Fayegheh
Dastjerdi, Zeinolabedin Sharifian
Shirzeyli, Maryam Hosseinzadeh
Salehi, Ensieh
author_facet Eini, Fatemeh
Kutenaei, Maryam Azizi
Zareei, Fayegheh
Dastjerdi, Zeinolabedin Sharifian
Shirzeyli, Maryam Hosseinzadeh
Salehi, Ensieh
author_sort Eini, Fatemeh
collection PubMed
description BACKGROUND: Although bacterial infections have been recognized as a possible cause of male infertility, the effect of bacterial infections on sperm quality and sperm DNA fragmentation remains controversial. The current study aimed to investigate the prevalence rate of bacterial infection in subfertile men and its effect on semen quality. Seminal fluid was collected from 172 male members of infertile couples attending the andrology infertility center and a group of 35 fertile subjects as a control. Sperm parameters and DNA fragmentation were evaluated based on the type of bacteria in all ejaculates. RESULTS: From the 172 patients investigated for infertility, 60 (34.88%) patients had a positive culture for pathogenic bacteria of different species. Leukocytospermia was significantly higher in infected samples in comparison with non-infected samples (p < 0.05). Sperm concentration and motility and morphology were significantly lower in infected than non-infected samples. Moreover, sperm DNA fragmentation was significantly higher in infected than non-infected samples. Besides, our results showed that sperm DNA fragmentation was correlated significantly with leukocytospermia (R: 0.22, p < 0.01). CONCLUSION: The present study suggested that bacterial infection significantly correlated with leukocytospermia could impair male fertility potential through decreasing sperm motility, morphology, and DNA integrity.
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spelling pubmed-83641162021-08-17 Effect of bacterial infection on sperm quality and DNA fragmentation in subfertile men with Leukocytospermia Eini, Fatemeh Kutenaei, Maryam Azizi Zareei, Fayegheh Dastjerdi, Zeinolabedin Sharifian Shirzeyli, Maryam Hosseinzadeh Salehi, Ensieh BMC Mol Cell Biol Research BACKGROUND: Although bacterial infections have been recognized as a possible cause of male infertility, the effect of bacterial infections on sperm quality and sperm DNA fragmentation remains controversial. The current study aimed to investigate the prevalence rate of bacterial infection in subfertile men and its effect on semen quality. Seminal fluid was collected from 172 male members of infertile couples attending the andrology infertility center and a group of 35 fertile subjects as a control. Sperm parameters and DNA fragmentation were evaluated based on the type of bacteria in all ejaculates. RESULTS: From the 172 patients investigated for infertility, 60 (34.88%) patients had a positive culture for pathogenic bacteria of different species. Leukocytospermia was significantly higher in infected samples in comparison with non-infected samples (p < 0.05). Sperm concentration and motility and morphology were significantly lower in infected than non-infected samples. Moreover, sperm DNA fragmentation was significantly higher in infected than non-infected samples. Besides, our results showed that sperm DNA fragmentation was correlated significantly with leukocytospermia (R: 0.22, p < 0.01). CONCLUSION: The present study suggested that bacterial infection significantly correlated with leukocytospermia could impair male fertility potential through decreasing sperm motility, morphology, and DNA integrity. BioMed Central 2021-08-13 /pmc/articles/PMC8364116/ /pubmed/34388964 http://dx.doi.org/10.1186/s12860-021-00380-8 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Eini, Fatemeh
Kutenaei, Maryam Azizi
Zareei, Fayegheh
Dastjerdi, Zeinolabedin Sharifian
Shirzeyli, Maryam Hosseinzadeh
Salehi, Ensieh
Effect of bacterial infection on sperm quality and DNA fragmentation in subfertile men with Leukocytospermia
title Effect of bacterial infection on sperm quality and DNA fragmentation in subfertile men with Leukocytospermia
title_full Effect of bacterial infection on sperm quality and DNA fragmentation in subfertile men with Leukocytospermia
title_fullStr Effect of bacterial infection on sperm quality and DNA fragmentation in subfertile men with Leukocytospermia
title_full_unstemmed Effect of bacterial infection on sperm quality and DNA fragmentation in subfertile men with Leukocytospermia
title_short Effect of bacterial infection on sperm quality and DNA fragmentation in subfertile men with Leukocytospermia
title_sort effect of bacterial infection on sperm quality and dna fragmentation in subfertile men with leukocytospermia
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364116/
https://www.ncbi.nlm.nih.gov/pubmed/34388964
http://dx.doi.org/10.1186/s12860-021-00380-8
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