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A paper-based colorimetric molecular test for SARS-CoV-2 in saliva

Herein, we describe the development of a paper-based device to detect nucleic acids of pathogens of interest in complex samples using loop-mediated isothermal amplification (LAMP) by producing a colorimetric response visible to the human eye. To demonstrate the utility of this device in emerging pub...

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Autores principales: Davidson, Josiah Levi, Wang, Jiangshan, Maruthamuthu, Murali Kannan, Dextre, Andres, Pascual-Garrigos, Ana, Mohan, Suraj, Putikam, Sai Venkata Sravan, Osman, Fujr Osman Ibrahim, McChesney, Darby, Seville, Jordan, Verma, Mohit S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by Elsevier B.V. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364207/
https://www.ncbi.nlm.nih.gov/pubmed/34423284
http://dx.doi.org/10.1016/j.biosx.2021.100076
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author Davidson, Josiah Levi
Wang, Jiangshan
Maruthamuthu, Murali Kannan
Dextre, Andres
Pascual-Garrigos, Ana
Mohan, Suraj
Putikam, Sai Venkata Sravan
Osman, Fujr Osman Ibrahim
McChesney, Darby
Seville, Jordan
Verma, Mohit S.
author_facet Davidson, Josiah Levi
Wang, Jiangshan
Maruthamuthu, Murali Kannan
Dextre, Andres
Pascual-Garrigos, Ana
Mohan, Suraj
Putikam, Sai Venkata Sravan
Osman, Fujr Osman Ibrahim
McChesney, Darby
Seville, Jordan
Verma, Mohit S.
author_sort Davidson, Josiah Levi
collection PubMed
description Herein, we describe the development of a paper-based device to detect nucleic acids of pathogens of interest in complex samples using loop-mediated isothermal amplification (LAMP) by producing a colorimetric response visible to the human eye. To demonstrate the utility of this device in emerging public health emergencies, we developed and optimized our device to detect SARS-CoV-2 in human saliva without preprocessing. The resulting device was capable of detecting the virus within 60 min and had an analytical sensitivity of 97% and a specificity of 100% with a limit of detection of 200 genomic copies/μL of patient saliva using image analysis. The device consists of a configurable number of reaction zones constructed of Grade 222 chromatography paper separated by 20 mil polystyrene spacers attached to a Melinex® backing via an ARclean® double-sided adhesive. The resulting device is easily configurable to detect multiple targets and has the potential to detect a variety of pathogens simply by changing the LAMP primer sets.
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spelling pubmed-83642072021-08-15 A paper-based colorimetric molecular test for SARS-CoV-2 in saliva Davidson, Josiah Levi Wang, Jiangshan Maruthamuthu, Murali Kannan Dextre, Andres Pascual-Garrigos, Ana Mohan, Suraj Putikam, Sai Venkata Sravan Osman, Fujr Osman Ibrahim McChesney, Darby Seville, Jordan Verma, Mohit S. Biosens Bioelectron X Article Herein, we describe the development of a paper-based device to detect nucleic acids of pathogens of interest in complex samples using loop-mediated isothermal amplification (LAMP) by producing a colorimetric response visible to the human eye. To demonstrate the utility of this device in emerging public health emergencies, we developed and optimized our device to detect SARS-CoV-2 in human saliva without preprocessing. The resulting device was capable of detecting the virus within 60 min and had an analytical sensitivity of 97% and a specificity of 100% with a limit of detection of 200 genomic copies/μL of patient saliva using image analysis. The device consists of a configurable number of reaction zones constructed of Grade 222 chromatography paper separated by 20 mil polystyrene spacers attached to a Melinex® backing via an ARclean® double-sided adhesive. The resulting device is easily configurable to detect multiple targets and has the potential to detect a variety of pathogens simply by changing the LAMP primer sets. The Authors. Published by Elsevier B.V. 2021-12 2021-08-14 /pmc/articles/PMC8364207/ /pubmed/34423284 http://dx.doi.org/10.1016/j.biosx.2021.100076 Text en © 2021 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Davidson, Josiah Levi
Wang, Jiangshan
Maruthamuthu, Murali Kannan
Dextre, Andres
Pascual-Garrigos, Ana
Mohan, Suraj
Putikam, Sai Venkata Sravan
Osman, Fujr Osman Ibrahim
McChesney, Darby
Seville, Jordan
Verma, Mohit S.
A paper-based colorimetric molecular test for SARS-CoV-2 in saliva
title A paper-based colorimetric molecular test for SARS-CoV-2 in saliva
title_full A paper-based colorimetric molecular test for SARS-CoV-2 in saliva
title_fullStr A paper-based colorimetric molecular test for SARS-CoV-2 in saliva
title_full_unstemmed A paper-based colorimetric molecular test for SARS-CoV-2 in saliva
title_short A paper-based colorimetric molecular test for SARS-CoV-2 in saliva
title_sort paper-based colorimetric molecular test for sars-cov-2 in saliva
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364207/
https://www.ncbi.nlm.nih.gov/pubmed/34423284
http://dx.doi.org/10.1016/j.biosx.2021.100076
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