Cargando…
A paper-based colorimetric molecular test for SARS-CoV-2 in saliva
Herein, we describe the development of a paper-based device to detect nucleic acids of pathogens of interest in complex samples using loop-mediated isothermal amplification (LAMP) by producing a colorimetric response visible to the human eye. To demonstrate the utility of this device in emerging pub...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Authors. Published by Elsevier B.V.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364207/ https://www.ncbi.nlm.nih.gov/pubmed/34423284 http://dx.doi.org/10.1016/j.biosx.2021.100076 |
_version_ | 1783738489630621696 |
---|---|
author | Davidson, Josiah Levi Wang, Jiangshan Maruthamuthu, Murali Kannan Dextre, Andres Pascual-Garrigos, Ana Mohan, Suraj Putikam, Sai Venkata Sravan Osman, Fujr Osman Ibrahim McChesney, Darby Seville, Jordan Verma, Mohit S. |
author_facet | Davidson, Josiah Levi Wang, Jiangshan Maruthamuthu, Murali Kannan Dextre, Andres Pascual-Garrigos, Ana Mohan, Suraj Putikam, Sai Venkata Sravan Osman, Fujr Osman Ibrahim McChesney, Darby Seville, Jordan Verma, Mohit S. |
author_sort | Davidson, Josiah Levi |
collection | PubMed |
description | Herein, we describe the development of a paper-based device to detect nucleic acids of pathogens of interest in complex samples using loop-mediated isothermal amplification (LAMP) by producing a colorimetric response visible to the human eye. To demonstrate the utility of this device in emerging public health emergencies, we developed and optimized our device to detect SARS-CoV-2 in human saliva without preprocessing. The resulting device was capable of detecting the virus within 60 min and had an analytical sensitivity of 97% and a specificity of 100% with a limit of detection of 200 genomic copies/μL of patient saliva using image analysis. The device consists of a configurable number of reaction zones constructed of Grade 222 chromatography paper separated by 20 mil polystyrene spacers attached to a Melinex® backing via an ARclean® double-sided adhesive. The resulting device is easily configurable to detect multiple targets and has the potential to detect a variety of pathogens simply by changing the LAMP primer sets. |
format | Online Article Text |
id | pubmed-8364207 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Authors. Published by Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83642072021-08-15 A paper-based colorimetric molecular test for SARS-CoV-2 in saliva Davidson, Josiah Levi Wang, Jiangshan Maruthamuthu, Murali Kannan Dextre, Andres Pascual-Garrigos, Ana Mohan, Suraj Putikam, Sai Venkata Sravan Osman, Fujr Osman Ibrahim McChesney, Darby Seville, Jordan Verma, Mohit S. Biosens Bioelectron X Article Herein, we describe the development of a paper-based device to detect nucleic acids of pathogens of interest in complex samples using loop-mediated isothermal amplification (LAMP) by producing a colorimetric response visible to the human eye. To demonstrate the utility of this device in emerging public health emergencies, we developed and optimized our device to detect SARS-CoV-2 in human saliva without preprocessing. The resulting device was capable of detecting the virus within 60 min and had an analytical sensitivity of 97% and a specificity of 100% with a limit of detection of 200 genomic copies/μL of patient saliva using image analysis. The device consists of a configurable number of reaction zones constructed of Grade 222 chromatography paper separated by 20 mil polystyrene spacers attached to a Melinex® backing via an ARclean® double-sided adhesive. The resulting device is easily configurable to detect multiple targets and has the potential to detect a variety of pathogens simply by changing the LAMP primer sets. The Authors. Published by Elsevier B.V. 2021-12 2021-08-14 /pmc/articles/PMC8364207/ /pubmed/34423284 http://dx.doi.org/10.1016/j.biosx.2021.100076 Text en © 2021 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Davidson, Josiah Levi Wang, Jiangshan Maruthamuthu, Murali Kannan Dextre, Andres Pascual-Garrigos, Ana Mohan, Suraj Putikam, Sai Venkata Sravan Osman, Fujr Osman Ibrahim McChesney, Darby Seville, Jordan Verma, Mohit S. A paper-based colorimetric molecular test for SARS-CoV-2 in saliva |
title | A paper-based colorimetric molecular test for SARS-CoV-2 in saliva |
title_full | A paper-based colorimetric molecular test for SARS-CoV-2 in saliva |
title_fullStr | A paper-based colorimetric molecular test for SARS-CoV-2 in saliva |
title_full_unstemmed | A paper-based colorimetric molecular test for SARS-CoV-2 in saliva |
title_short | A paper-based colorimetric molecular test for SARS-CoV-2 in saliva |
title_sort | paper-based colorimetric molecular test for sars-cov-2 in saliva |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364207/ https://www.ncbi.nlm.nih.gov/pubmed/34423284 http://dx.doi.org/10.1016/j.biosx.2021.100076 |
work_keys_str_mv | AT davidsonjosiahlevi apaperbasedcolorimetricmoleculartestforsarscov2insaliva AT wangjiangshan apaperbasedcolorimetricmoleculartestforsarscov2insaliva AT maruthamuthumuralikannan apaperbasedcolorimetricmoleculartestforsarscov2insaliva AT dextreandres apaperbasedcolorimetricmoleculartestforsarscov2insaliva AT pascualgarrigosana apaperbasedcolorimetricmoleculartestforsarscov2insaliva AT mohansuraj apaperbasedcolorimetricmoleculartestforsarscov2insaliva AT putikamsaivenkatasravan apaperbasedcolorimetricmoleculartestforsarscov2insaliva AT osmanfujrosmanibrahim apaperbasedcolorimetricmoleculartestforsarscov2insaliva AT mcchesneydarby apaperbasedcolorimetricmoleculartestforsarscov2insaliva AT sevillejordan apaperbasedcolorimetricmoleculartestforsarscov2insaliva AT vermamohits apaperbasedcolorimetricmoleculartestforsarscov2insaliva AT davidsonjosiahlevi paperbasedcolorimetricmoleculartestforsarscov2insaliva AT wangjiangshan paperbasedcolorimetricmoleculartestforsarscov2insaliva AT maruthamuthumuralikannan paperbasedcolorimetricmoleculartestforsarscov2insaliva AT dextreandres paperbasedcolorimetricmoleculartestforsarscov2insaliva AT pascualgarrigosana paperbasedcolorimetricmoleculartestforsarscov2insaliva AT mohansuraj paperbasedcolorimetricmoleculartestforsarscov2insaliva AT putikamsaivenkatasravan paperbasedcolorimetricmoleculartestforsarscov2insaliva AT osmanfujrosmanibrahim paperbasedcolorimetricmoleculartestforsarscov2insaliva AT mcchesneydarby paperbasedcolorimetricmoleculartestforsarscov2insaliva AT sevillejordan paperbasedcolorimetricmoleculartestforsarscov2insaliva AT vermamohits paperbasedcolorimetricmoleculartestforsarscov2insaliva |