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Different DNA-binding specificities of NLP and NIN transcription factors underlie nitrate-induced control of root nodulation
Leguminous plants produce nodules for nitrogen fixation; however, nodule production incurs an energy cost. Therefore, as an adaptive strategy, leguminous plants halt root nodule development when sufficient amounts of nitrogen nutrients, such as nitrate, are present in the environment. Although legum...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364233/ https://www.ncbi.nlm.nih.gov/pubmed/33826745 http://dx.doi.org/10.1093/plcell/koab103 |
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author | Nishida, Hanna Nosaki, Shohei Suzuki, Takamasa Ito, Momoyo Miyakawa, Takuya Nomoto, Mika Tada, Yasuomi Miura, Kenji Tanokura, Masaru Kawaguchi, Masayoshi Suzaki, Takuya |
author_facet | Nishida, Hanna Nosaki, Shohei Suzuki, Takamasa Ito, Momoyo Miyakawa, Takuya Nomoto, Mika Tada, Yasuomi Miura, Kenji Tanokura, Masaru Kawaguchi, Masayoshi Suzaki, Takuya |
author_sort | Nishida, Hanna |
collection | PubMed |
description | Leguminous plants produce nodules for nitrogen fixation; however, nodule production incurs an energy cost. Therefore, as an adaptive strategy, leguminous plants halt root nodule development when sufficient amounts of nitrogen nutrients, such as nitrate, are present in the environment. Although legume NODULE INCEPTION (NIN)-LIKE PROTEIN (NLP) transcription factors have recently been identified, understanding how nodulation is controlled by nitrate, a fundamental question for nitrate-mediated transcriptional regulation of symbiotic genes, remains elusive. Here, we show that two Lotus japonicus NLPs, NITRATE UNRESPONSIVE SYMBIOSIS 1 (NRSYM1)/LjNLP4 and NRSYM2/LjNLP1, have overlapping functions in the nitrate-induced control of nodulation and act as master regulators for nitrate-dependent gene expression. We further identify candidate target genes of LjNLP4 by combining transcriptome analysis with a DNA affinity purification-seq approach. We then demonstrate that LjNLP4 and LjNIN, a key nodulation-specific regulator and paralog of LjNLP4, have different DNA-binding specificities. Moreover, LjNLP4–LjNIN dimerization underlies LjNLP4-mediated bifunctional transcriptional regulation. These data provide a basic principle for how nitrate controls nodulation through positive and negative regulation of symbiotic genes. |
format | Online Article Text |
id | pubmed-8364233 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-83642332021-08-17 Different DNA-binding specificities of NLP and NIN transcription factors underlie nitrate-induced control of root nodulation Nishida, Hanna Nosaki, Shohei Suzuki, Takamasa Ito, Momoyo Miyakawa, Takuya Nomoto, Mika Tada, Yasuomi Miura, Kenji Tanokura, Masaru Kawaguchi, Masayoshi Suzaki, Takuya Plant Cell Research Articles Leguminous plants produce nodules for nitrogen fixation; however, nodule production incurs an energy cost. Therefore, as an adaptive strategy, leguminous plants halt root nodule development when sufficient amounts of nitrogen nutrients, such as nitrate, are present in the environment. Although legume NODULE INCEPTION (NIN)-LIKE PROTEIN (NLP) transcription factors have recently been identified, understanding how nodulation is controlled by nitrate, a fundamental question for nitrate-mediated transcriptional regulation of symbiotic genes, remains elusive. Here, we show that two Lotus japonicus NLPs, NITRATE UNRESPONSIVE SYMBIOSIS 1 (NRSYM1)/LjNLP4 and NRSYM2/LjNLP1, have overlapping functions in the nitrate-induced control of nodulation and act as master regulators for nitrate-dependent gene expression. We further identify candidate target genes of LjNLP4 by combining transcriptome analysis with a DNA affinity purification-seq approach. We then demonstrate that LjNLP4 and LjNIN, a key nodulation-specific regulator and paralog of LjNLP4, have different DNA-binding specificities. Moreover, LjNLP4–LjNIN dimerization underlies LjNLP4-mediated bifunctional transcriptional regulation. These data provide a basic principle for how nitrate controls nodulation through positive and negative regulation of symbiotic genes. Oxford University Press 2021-04-07 /pmc/articles/PMC8364233/ /pubmed/33826745 http://dx.doi.org/10.1093/plcell/koab103 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Research Articles Nishida, Hanna Nosaki, Shohei Suzuki, Takamasa Ito, Momoyo Miyakawa, Takuya Nomoto, Mika Tada, Yasuomi Miura, Kenji Tanokura, Masaru Kawaguchi, Masayoshi Suzaki, Takuya Different DNA-binding specificities of NLP and NIN transcription factors underlie nitrate-induced control of root nodulation |
title | Different DNA-binding specificities of NLP and NIN transcription factors underlie nitrate-induced control of root nodulation |
title_full | Different DNA-binding specificities of NLP and NIN transcription factors underlie nitrate-induced control of root nodulation |
title_fullStr | Different DNA-binding specificities of NLP and NIN transcription factors underlie nitrate-induced control of root nodulation |
title_full_unstemmed | Different DNA-binding specificities of NLP and NIN transcription factors underlie nitrate-induced control of root nodulation |
title_short | Different DNA-binding specificities of NLP and NIN transcription factors underlie nitrate-induced control of root nodulation |
title_sort | different dna-binding specificities of nlp and nin transcription factors underlie nitrate-induced control of root nodulation |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364233/ https://www.ncbi.nlm.nih.gov/pubmed/33826745 http://dx.doi.org/10.1093/plcell/koab103 |
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