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Effect of storage conditions on SARS-CoV-2 RNA quantification in wastewater solids

SARS-CoV-2 RNA in wastewater settled solids is associated with COVID-19 incidence in sewersheds and therefore, there is a strong interest in using these measurements to augment traditional disease surveillance methods. A wastewater surveillance program should provide rapid turn around for sample mea...

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Autores principales: Simpson, Adrian, Topol, Aaron, White, Bradley J., Wolfe, Marlene K., Wigginton, Krista R., Boehm, Alexandria B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364322/
https://www.ncbi.nlm.nih.gov/pubmed/34447628
http://dx.doi.org/10.7717/peerj.11933
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author Simpson, Adrian
Topol, Aaron
White, Bradley J.
Wolfe, Marlene K.
Wigginton, Krista R.
Boehm, Alexandria B.
author_facet Simpson, Adrian
Topol, Aaron
White, Bradley J.
Wolfe, Marlene K.
Wigginton, Krista R.
Boehm, Alexandria B.
author_sort Simpson, Adrian
collection PubMed
description SARS-CoV-2 RNA in wastewater settled solids is associated with COVID-19 incidence in sewersheds and therefore, there is a strong interest in using these measurements to augment traditional disease surveillance methods. A wastewater surveillance program should provide rapid turn around for sample measurements (ideally within 24 hours), but storage of samples is necessary for a variety of reasons including biobanking. Here we investigate how storage of wastewater solids at 4 °C, −20 °C, and −80 °C affects measured concentrations of SARS-CoV-2 RNA. We find that short term (7 or 8 d) storage of raw solids at 4 °C has little effect on measured concentrations of SARS-CoV-2 RNA, whereas longer term storage at 4 °C (35–122 d) or freezing reduces measurements by 60%, on average. We show that normalizing SARS-CoV-2 RNA concentrations by concentrations of pepper mild mottle virus (PMMoV) RNA, an endogenous wastewater virus, can correct for changes during storage as storage can have a similar effect on PMMoV RNA as on SARS-CoV-2 RNA. The reductions in SARS-CoV-2 RNA in solids during freeze thaws is less than those reported for the same target in liquid influent by several authors.
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spelling pubmed-83643222021-08-25 Effect of storage conditions on SARS-CoV-2 RNA quantification in wastewater solids Simpson, Adrian Topol, Aaron White, Bradley J. Wolfe, Marlene K. Wigginton, Krista R. Boehm, Alexandria B. PeerJ Virology SARS-CoV-2 RNA in wastewater settled solids is associated with COVID-19 incidence in sewersheds and therefore, there is a strong interest in using these measurements to augment traditional disease surveillance methods. A wastewater surveillance program should provide rapid turn around for sample measurements (ideally within 24 hours), but storage of samples is necessary for a variety of reasons including biobanking. Here we investigate how storage of wastewater solids at 4 °C, −20 °C, and −80 °C affects measured concentrations of SARS-CoV-2 RNA. We find that short term (7 or 8 d) storage of raw solids at 4 °C has little effect on measured concentrations of SARS-CoV-2 RNA, whereas longer term storage at 4 °C (35–122 d) or freezing reduces measurements by 60%, on average. We show that normalizing SARS-CoV-2 RNA concentrations by concentrations of pepper mild mottle virus (PMMoV) RNA, an endogenous wastewater virus, can correct for changes during storage as storage can have a similar effect on PMMoV RNA as on SARS-CoV-2 RNA. The reductions in SARS-CoV-2 RNA in solids during freeze thaws is less than those reported for the same target in liquid influent by several authors. PeerJ Inc. 2021-08-11 /pmc/articles/PMC8364322/ /pubmed/34447628 http://dx.doi.org/10.7717/peerj.11933 Text en ©2021 Simpson et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Virology
Simpson, Adrian
Topol, Aaron
White, Bradley J.
Wolfe, Marlene K.
Wigginton, Krista R.
Boehm, Alexandria B.
Effect of storage conditions on SARS-CoV-2 RNA quantification in wastewater solids
title Effect of storage conditions on SARS-CoV-2 RNA quantification in wastewater solids
title_full Effect of storage conditions on SARS-CoV-2 RNA quantification in wastewater solids
title_fullStr Effect of storage conditions on SARS-CoV-2 RNA quantification in wastewater solids
title_full_unstemmed Effect of storage conditions on SARS-CoV-2 RNA quantification in wastewater solids
title_short Effect of storage conditions on SARS-CoV-2 RNA quantification in wastewater solids
title_sort effect of storage conditions on sars-cov-2 rna quantification in wastewater solids
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364322/
https://www.ncbi.nlm.nih.gov/pubmed/34447628
http://dx.doi.org/10.7717/peerj.11933
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