Hydrogen Attenuates Myocardial Injury in Rats by Regulating Oxidative Stress and NLRP3 Inflammasome Mediated Pyroptosis

Purpose: Hydrogen (H(2)) is an antioxidant with anti-inflammatory and apoptosis functions.This study aimed to estimate the effects of H(2) on acute myocardial infarction (AMI) in rats and its association with the inhibition of oxidative stress and cardiomyocyte pyroptosis. Methods: Sixty-four rats were randomly divided into three groups (Sham, AMI, and H(2)). The left anterior descending coronary artery (LAD) of rats in the AMI and H(2) groups was ligated, while rats in the Sham group were threaded without ligation. In addition, 2% H(2) was administered by inhalation for 24 h after ligation in the H(2) group. Transthoracic echocardiography was performed after H(2) inhalation, followed by collection of the serum and cardiac tissue of all rats. Results: H(2) inhalation ameliorated the cardiac dysfunction, infarct size and inflammatory cell infiltration caused by AMI. Meanwhile, H(2) inhalation reduced the concentration of serum Troponin I (TnI), brain natriuretic peptide (BNP), reactive oxygen species (ROS), cardiac malondialdehyde (MDA), and 8-OHdG. In addition, H(2) inhalation inhibited cardiac inflammation and pyroptosis relative proteins expression. Conclusion: H(2) effectively promoted heart functions in AMI rats by regulating oxidative stress and pyroptosis.