IGF2BP2-modified circular RNA circARHGAP12 promotes cervical cancer progression by interacting m(6)A/FOXM1 manner

Emerging evidence indicates that circular RNA (circRNA) and N(6)-methyladenosine (m(6)A) play critical roles in cervical cancer. However, the synergistic effect of circRNA and m(6)A on cervical cancer progression is unclear. In the present study, our sequencing data revealed that a novel m(6)A-modif...

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Autores principales: Ji, Fei, Lu, Yang, Chen, Shaoyun, Yu, Yan, Lin, Xiaoling, Zhu, Yuanfang, Luo, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364552/
https://www.ncbi.nlm.nih.gov/pubmed/34392306
http://dx.doi.org/10.1038/s41420-021-00595-w
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author Ji, Fei
Lu, Yang
Chen, Shaoyun
Yu, Yan
Lin, Xiaoling
Zhu, Yuanfang
Luo, Xin
author_facet Ji, Fei
Lu, Yang
Chen, Shaoyun
Yu, Yan
Lin, Xiaoling
Zhu, Yuanfang
Luo, Xin
author_sort Ji, Fei
collection PubMed
description Emerging evidence indicates that circular RNA (circRNA) and N(6)-methyladenosine (m(6)A) play critical roles in cervical cancer. However, the synergistic effect of circRNA and m(6)A on cervical cancer progression is unclear. In the present study, our sequencing data revealed that a novel m(6)A-modified circRNA (circARHGAP12, hsa_circ_0000231) upregulated in the cervical cancer tissue and cells. Interestingly, the m(6)A modification of circARHGAP12 could amplify its enrichment. Functional experiments illustrated that circARHGAP12 promoted the tumor progression of cervical cancer in vivo and vitro. Furthermore, MeRIP-Seq illustrated that there was a remarkable m(6)A site in FOXM1 mRNA. CircARHGAP12 interacted with m(6)A reader IGF2BP2 to combine with FOXM1 mRNA, thereby accelerating the stability of FOXM1 mRNA. In conclusion, we found that circARHGAP12 exerted the oncogenic role in cervical cancer progression through m(6)A-dependent IGF2BP2/FOXM1 pathway. These findings may provide new concepts for cervical cancer biology and pathological physiology.
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spelling pubmed-83645522021-08-31 IGF2BP2-modified circular RNA circARHGAP12 promotes cervical cancer progression by interacting m(6)A/FOXM1 manner Ji, Fei Lu, Yang Chen, Shaoyun Yu, Yan Lin, Xiaoling Zhu, Yuanfang Luo, Xin Cell Death Discov Article Emerging evidence indicates that circular RNA (circRNA) and N(6)-methyladenosine (m(6)A) play critical roles in cervical cancer. However, the synergistic effect of circRNA and m(6)A on cervical cancer progression is unclear. In the present study, our sequencing data revealed that a novel m(6)A-modified circRNA (circARHGAP12, hsa_circ_0000231) upregulated in the cervical cancer tissue and cells. Interestingly, the m(6)A modification of circARHGAP12 could amplify its enrichment. Functional experiments illustrated that circARHGAP12 promoted the tumor progression of cervical cancer in vivo and vitro. Furthermore, MeRIP-Seq illustrated that there was a remarkable m(6)A site in FOXM1 mRNA. CircARHGAP12 interacted with m(6)A reader IGF2BP2 to combine with FOXM1 mRNA, thereby accelerating the stability of FOXM1 mRNA. In conclusion, we found that circARHGAP12 exerted the oncogenic role in cervical cancer progression through m(6)A-dependent IGF2BP2/FOXM1 pathway. These findings may provide new concepts for cervical cancer biology and pathological physiology. Nature Publishing Group UK 2021-08-14 /pmc/articles/PMC8364552/ /pubmed/34392306 http://dx.doi.org/10.1038/s41420-021-00595-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Ji, Fei
Lu, Yang
Chen, Shaoyun
Yu, Yan
Lin, Xiaoling
Zhu, Yuanfang
Luo, Xin
IGF2BP2-modified circular RNA circARHGAP12 promotes cervical cancer progression by interacting m(6)A/FOXM1 manner
title IGF2BP2-modified circular RNA circARHGAP12 promotes cervical cancer progression by interacting m(6)A/FOXM1 manner
title_full IGF2BP2-modified circular RNA circARHGAP12 promotes cervical cancer progression by interacting m(6)A/FOXM1 manner
title_fullStr IGF2BP2-modified circular RNA circARHGAP12 promotes cervical cancer progression by interacting m(6)A/FOXM1 manner
title_full_unstemmed IGF2BP2-modified circular RNA circARHGAP12 promotes cervical cancer progression by interacting m(6)A/FOXM1 manner
title_short IGF2BP2-modified circular RNA circARHGAP12 promotes cervical cancer progression by interacting m(6)A/FOXM1 manner
title_sort igf2bp2-modified circular rna circarhgap12 promotes cervical cancer progression by interacting m(6)a/foxm1 manner
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364552/
https://www.ncbi.nlm.nih.gov/pubmed/34392306
http://dx.doi.org/10.1038/s41420-021-00595-w
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