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Mutation SVCT2 promotes cell proliferation, invasion and migration in colorectal cancer
The sodium-dependent vitamin C transporter 2 (SVCT2) surface glycoprotein regulates ascorbate accumulation in the plasma, often resulting in the induction of cancer cell death. Therefore, high expression of this gene associates with increased overall survival in several cancers. However, in colorect...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364649/ https://www.ncbi.nlm.nih.gov/pubmed/34405001 http://dx.doi.org/10.7150/jca.57463 |
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author | Park, Sang-Soo Ryu, Yea Seong Koh, Dong-In Hong, Seung-Woo Moon, Jai-Hee Shin, Jae-Sik Kim, Mi Jin Kim, Do Yeon Hong, Jun Ki Kim, Eun Ho Jeong, Hong-Rae Park, Yoon Sun Kim, Joseph Kim, Dong Min Yun, Hyeseon Shin, Joo-Yeon Jin, Dong-Hoon |
author_facet | Park, Sang-Soo Ryu, Yea Seong Koh, Dong-In Hong, Seung-Woo Moon, Jai-Hee Shin, Jae-Sik Kim, Mi Jin Kim, Do Yeon Hong, Jun Ki Kim, Eun Ho Jeong, Hong-Rae Park, Yoon Sun Kim, Joseph Kim, Dong Min Yun, Hyeseon Shin, Joo-Yeon Jin, Dong-Hoon |
author_sort | Park, Sang-Soo |
collection | PubMed |
description | The sodium-dependent vitamin C transporter 2 (SVCT2) surface glycoprotein regulates ascorbate accumulation in the plasma, often resulting in the induction of cancer cell death. Therefore, high expression of this gene associates with increased overall survival in several cancers. However, in colorectal cancer (CRC), high (likely mutated) SVCT2 expression relates to poor overall survival, and its functional significance has not been studied. Thus, we hypothesize that mutant SVCT2 expression could affect CRC patient survival. According to biological databases, SVCT2 has been found to be mutated frequently, and SVCT2 E264K has a particularly high pathogenic score (0.98), compared to other SVCT2 mutant sites, in CRC patients. Interestingly, our results reveal expression of SVCT2 E264K in many CRC tissues and cells. Also, we found wild-type SVCT2 expression to be largely localized to the cytoplasm and membrane, while SVCT2 E264K was restricted to the cytoplasm. We further found that SVCT2 E264K overexpression increases cell growth. By contrast, SVCT2 E264K knockdown significantly reduced cell proliferation and promoted cell apoptosis, resulting in inhibition of cell invasion and migration. Taken together, SVCT2 E264K plays a critical role in proliferation in CRC. Our results suggest that SVCT2 E264K could be a promising novel therapeutic target in CRC. |
format | Online Article Text |
id | pubmed-8364649 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-83646492021-08-16 Mutation SVCT2 promotes cell proliferation, invasion and migration in colorectal cancer Park, Sang-Soo Ryu, Yea Seong Koh, Dong-In Hong, Seung-Woo Moon, Jai-Hee Shin, Jae-Sik Kim, Mi Jin Kim, Do Yeon Hong, Jun Ki Kim, Eun Ho Jeong, Hong-Rae Park, Yoon Sun Kim, Joseph Kim, Dong Min Yun, Hyeseon Shin, Joo-Yeon Jin, Dong-Hoon J Cancer Research Paper The sodium-dependent vitamin C transporter 2 (SVCT2) surface glycoprotein regulates ascorbate accumulation in the plasma, often resulting in the induction of cancer cell death. Therefore, high expression of this gene associates with increased overall survival in several cancers. However, in colorectal cancer (CRC), high (likely mutated) SVCT2 expression relates to poor overall survival, and its functional significance has not been studied. Thus, we hypothesize that mutant SVCT2 expression could affect CRC patient survival. According to biological databases, SVCT2 has been found to be mutated frequently, and SVCT2 E264K has a particularly high pathogenic score (0.98), compared to other SVCT2 mutant sites, in CRC patients. Interestingly, our results reveal expression of SVCT2 E264K in many CRC tissues and cells. Also, we found wild-type SVCT2 expression to be largely localized to the cytoplasm and membrane, while SVCT2 E264K was restricted to the cytoplasm. We further found that SVCT2 E264K overexpression increases cell growth. By contrast, SVCT2 E264K knockdown significantly reduced cell proliferation and promoted cell apoptosis, resulting in inhibition of cell invasion and migration. Taken together, SVCT2 E264K plays a critical role in proliferation in CRC. Our results suggest that SVCT2 E264K could be a promising novel therapeutic target in CRC. Ivyspring International Publisher 2021-07-06 /pmc/articles/PMC8364649/ /pubmed/34405001 http://dx.doi.org/10.7150/jca.57463 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Park, Sang-Soo Ryu, Yea Seong Koh, Dong-In Hong, Seung-Woo Moon, Jai-Hee Shin, Jae-Sik Kim, Mi Jin Kim, Do Yeon Hong, Jun Ki Kim, Eun Ho Jeong, Hong-Rae Park, Yoon Sun Kim, Joseph Kim, Dong Min Yun, Hyeseon Shin, Joo-Yeon Jin, Dong-Hoon Mutation SVCT2 promotes cell proliferation, invasion and migration in colorectal cancer |
title | Mutation SVCT2 promotes cell proliferation, invasion and migration in colorectal cancer |
title_full | Mutation SVCT2 promotes cell proliferation, invasion and migration in colorectal cancer |
title_fullStr | Mutation SVCT2 promotes cell proliferation, invasion and migration in colorectal cancer |
title_full_unstemmed | Mutation SVCT2 promotes cell proliferation, invasion and migration in colorectal cancer |
title_short | Mutation SVCT2 promotes cell proliferation, invasion and migration in colorectal cancer |
title_sort | mutation svct2 promotes cell proliferation, invasion and migration in colorectal cancer |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364649/ https://www.ncbi.nlm.nih.gov/pubmed/34405001 http://dx.doi.org/10.7150/jca.57463 |
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