Rapid Base-Specific Calling of SARS-CoV-2 Variants of Concern Using Combined RT-PCR Melting Curve Screening and SIRPH Technology

BACKGROUND: The emergence of novel variants of concern of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) demands fast and reliable detection of such variants in local populations. METHODS: Here we present a cost-efficient and fast workflow combining a prescreening of SARS-CoV-2-positiv...

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Detalles Bibliográficos
Autores principales: Tierling, Sascha, Kattler, Kathrin, Vogelgesang, Markus, Pfuhl, Thorsten, Lohse, Stefan, Lo Porto, Christina, Schmitt, Beate, Nastasja, Seiwert, Salhab, Abdulrahman, Smola, Sigrun, Walter, Jörn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Major Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364759/
https://www.ncbi.nlm.nih.gov/pubmed/34409122
http://dx.doi.org/10.1093/ofid/ofab364
Descripción
Sumario:BACKGROUND: The emergence of novel variants of concern of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) demands fast and reliable detection of such variants in local populations. METHODS: Here we present a cost-efficient and fast workflow combining a prescreening of SARS-CoV-2-positive samples using reverse transcription polymerase chain reaction melting curve analysis with multiplexed IP-RP-HPLC-based single nucleotide primer extensions. RESULTS: The entire workflow from positive SARS-CoV-2 testing to base-specific identification of variants requires about 24 hours. CONCLUSIONS: We applied the sensitive method to monitor local variant of concern outbreaks in SARS-CoV-2-positive samples collected in a confined region of Germany.

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