Single base mutations in the nucleocapsid gene of SARS-CoV-2 affects amplification efficiency of sequence variants and may lead to assay failure

Reverse transcriptase quantitative PCR (RT-qPCR) is the main diagnostic assay used to detect SARS-CoV-2 RNA in respiratory samples. RT-qPCR is performed by specifically targeting the viral genome using complementary oligonucleotides called primers and probes. This approach relies on prior knowledge...

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Detalles Bibliográficos
Autores principales: Storey, Nathaniel, Brown, Julianne R, Pereira, Rui P A, O'Sullivan, Denise M, Huggett, Jim F, Williams, Rachel, Breuer, Judith, Harris, Kathryn A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by Elsevier Ltd. 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364770/
https://www.ncbi.nlm.nih.gov/pubmed/35262020
http://dx.doi.org/10.1016/j.jcvp.2021.100037

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