EBiSC best practice: How to ensure optimal generation, qualification, and distribution of iPSC lines

Disease-relevant human induced pluripotent stem cells (iPSCs) are generated worldwide for research purposes; however, without robust and practical ethical, legal, and quality standards, there is a high risk that their true potential will not be realized. Best practices for tissue procurement, iPSC r...

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Detalles Bibliográficos
Autores principales: Steeg, Rachel, Mueller, Sabine C., Mah, Nancy, Holst, Bjørn, Cabrera-Socorro, Alfredo, Stacey, Glyn N., De Sousa, Paul A., Courtney, Aidan, Zimmermann, Heiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Perspective
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365092/
https://www.ncbi.nlm.nih.gov/pubmed/34380020
http://dx.doi.org/10.1016/j.stemcr.2021.07.009
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author Steeg, Rachel
Mueller, Sabine C.
Mah, Nancy
Holst, Bjørn
Cabrera-Socorro, Alfredo
Stacey, Glyn N.
De Sousa, Paul A.
Courtney, Aidan
Zimmermann, Heiko
author_facet Steeg, Rachel
Mueller, Sabine C.
Mah, Nancy
Holst, Bjørn
Cabrera-Socorro, Alfredo
Stacey, Glyn N.
De Sousa, Paul A.
Courtney, Aidan
Zimmermann, Heiko
author_sort Steeg, Rachel
collection PubMed
description Disease-relevant human induced pluripotent stem cells (iPSCs) are generated worldwide for research purposes; however, without robust and practical ethical, legal, and quality standards, there is a high risk that their true potential will not be realized. Best practices for tissue procurement, iPSC reprogramming, day-to-day cultivation, quality control, and data management aligned with an ethical and legal framework must be included into daily operations to ensure their promise is maximized. Here we discuss key learning experiences from 7 years of operating the European Bank for induced Pluripotent Stem Cells (EBiSC) and recommend how to incorporate solutions into a daily management framework.
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spelling pubmed-83650922021-08-23 EBiSC best practice: How to ensure optimal generation, qualification, and distribution of iPSC lines Steeg, Rachel Mueller, Sabine C. Mah, Nancy Holst, Bjørn Cabrera-Socorro, Alfredo Stacey, Glyn N. De Sousa, Paul A. Courtney, Aidan Zimmermann, Heiko Stem Cell Reports Perspective Disease-relevant human induced pluripotent stem cells (iPSCs) are generated worldwide for research purposes; however, without robust and practical ethical, legal, and quality standards, there is a high risk that their true potential will not be realized. Best practices for tissue procurement, iPSC reprogramming, day-to-day cultivation, quality control, and data management aligned with an ethical and legal framework must be included into daily operations to ensure their promise is maximized. Here we discuss key learning experiences from 7 years of operating the European Bank for induced Pluripotent Stem Cells (EBiSC) and recommend how to incorporate solutions into a daily management framework. Elsevier 2021-08-10 /pmc/articles/PMC8365092/ /pubmed/34380020 http://dx.doi.org/10.1016/j.stemcr.2021.07.009 Text en © 2021 Fraunhofer Institut fû¥r Biomedizinische Technik https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Perspective
Steeg, Rachel
Mueller, Sabine C.
Mah, Nancy
Holst, Bjørn
Cabrera-Socorro, Alfredo
Stacey, Glyn N.
De Sousa, Paul A.
Courtney, Aidan
Zimmermann, Heiko
EBiSC best practice: How to ensure optimal generation, qualification, and distribution of iPSC lines
title EBiSC best practice: How to ensure optimal generation, qualification, and distribution of iPSC lines
title_full EBiSC best practice: How to ensure optimal generation, qualification, and distribution of iPSC lines
title_fullStr EBiSC best practice: How to ensure optimal generation, qualification, and distribution of iPSC lines
title_full_unstemmed EBiSC best practice: How to ensure optimal generation, qualification, and distribution of iPSC lines
title_short EBiSC best practice: How to ensure optimal generation, qualification, and distribution of iPSC lines
title_sort ebisc best practice: how to ensure optimal generation, qualification, and distribution of ipsc lines
topic Perspective
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365092/
https://www.ncbi.nlm.nih.gov/pubmed/34380020
http://dx.doi.org/10.1016/j.stemcr.2021.07.009
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