Cargando…
Comprehensive dataset to assess morphological changes subsequent to bleomycin exposure
Intratracheal bleomycin causes pulmonary injury, inflammation and fibrosis. The characteristic patchy nature of the injury makes analysis challenging. Histological assessment of lung injury is a useful tool to evaluate damage, however quantification is not standardized. We propose a multi-factorial...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365314/ https://www.ncbi.nlm.nih.gov/pubmed/34430679 http://dx.doi.org/10.1016/j.dib.2021.107270 |
_version_ | 1783738683464089600 |
---|---|
author | Golden, Thea Murray, Alexa Venosa, Alessandro Gow, Andrew J. |
author_facet | Golden, Thea Murray, Alexa Venosa, Alessandro Gow, Andrew J. |
author_sort | Golden, Thea |
collection | PubMed |
description | Intratracheal bleomycin causes pulmonary injury, inflammation and fibrosis. The characteristic patchy nature of the injury makes analysis challenging. Histological assessment of lung injury is a useful tool to evaluate damage, however quantification is not standardized. We propose a multi-factorial approach to assess morphological changes subsequent to intratracheal bleomycin mediated lung injury. Lungs were inflation fixed with paraformaldehyde, sectioned and stained with hematoxylin and eosin. Whole slide images were scanned and ten 400x images were randomly chosen throughout the tissue for further analysis. Using ImageJ software, alveolar wall width was measured, nuclei were counted and airspace was quantified. Morphological changes were identified in mice instilled with bleomycin. This combination offers a robust measure of lung morphology especially in a heterogenous injury. |
format | Online Article Text |
id | pubmed-8365314 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-83653142021-08-23 Comprehensive dataset to assess morphological changes subsequent to bleomycin exposure Golden, Thea Murray, Alexa Venosa, Alessandro Gow, Andrew J. Data Brief Data Article Intratracheal bleomycin causes pulmonary injury, inflammation and fibrosis. The characteristic patchy nature of the injury makes analysis challenging. Histological assessment of lung injury is a useful tool to evaluate damage, however quantification is not standardized. We propose a multi-factorial approach to assess morphological changes subsequent to intratracheal bleomycin mediated lung injury. Lungs were inflation fixed with paraformaldehyde, sectioned and stained with hematoxylin and eosin. Whole slide images were scanned and ten 400x images were randomly chosen throughout the tissue for further analysis. Using ImageJ software, alveolar wall width was measured, nuclei were counted and airspace was quantified. Morphological changes were identified in mice instilled with bleomycin. This combination offers a robust measure of lung morphology especially in a heterogenous injury. Elsevier 2021-08-05 /pmc/articles/PMC8365314/ /pubmed/34430679 http://dx.doi.org/10.1016/j.dib.2021.107270 Text en © 2021 The Authors. Published by Elsevier Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Data Article Golden, Thea Murray, Alexa Venosa, Alessandro Gow, Andrew J. Comprehensive dataset to assess morphological changes subsequent to bleomycin exposure |
title | Comprehensive dataset to assess morphological changes subsequent to bleomycin exposure |
title_full | Comprehensive dataset to assess morphological changes subsequent to bleomycin exposure |
title_fullStr | Comprehensive dataset to assess morphological changes subsequent to bleomycin exposure |
title_full_unstemmed | Comprehensive dataset to assess morphological changes subsequent to bleomycin exposure |
title_short | Comprehensive dataset to assess morphological changes subsequent to bleomycin exposure |
title_sort | comprehensive dataset to assess morphological changes subsequent to bleomycin exposure |
topic | Data Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365314/ https://www.ncbi.nlm.nih.gov/pubmed/34430679 http://dx.doi.org/10.1016/j.dib.2021.107270 |
work_keys_str_mv | AT goldenthea comprehensivedatasettoassessmorphologicalchangessubsequenttobleomycinexposure AT murrayalexa comprehensivedatasettoassessmorphologicalchangessubsequenttobleomycinexposure AT venosaalessandro comprehensivedatasettoassessmorphologicalchangessubsequenttobleomycinexposure AT gowandrewj comprehensivedatasettoassessmorphologicalchangessubsequenttobleomycinexposure |