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Comprehensive dataset to assess morphological changes subsequent to bleomycin exposure

Intratracheal bleomycin causes pulmonary injury, inflammation and fibrosis. The characteristic patchy nature of the injury makes analysis challenging. Histological assessment of lung injury is a useful tool to evaluate damage, however quantification is not standardized. We propose a multi-factorial...

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Detalles Bibliográficos
Autores principales: Golden, Thea, Murray, Alexa, Venosa, Alessandro, Gow, Andrew J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365314/
https://www.ncbi.nlm.nih.gov/pubmed/34430679
http://dx.doi.org/10.1016/j.dib.2021.107270
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author Golden, Thea
Murray, Alexa
Venosa, Alessandro
Gow, Andrew J.
author_facet Golden, Thea
Murray, Alexa
Venosa, Alessandro
Gow, Andrew J.
author_sort Golden, Thea
collection PubMed
description Intratracheal bleomycin causes pulmonary injury, inflammation and fibrosis. The characteristic patchy nature of the injury makes analysis challenging. Histological assessment of lung injury is a useful tool to evaluate damage, however quantification is not standardized. We propose a multi-factorial approach to assess morphological changes subsequent to intratracheal bleomycin mediated lung injury. Lungs were inflation fixed with paraformaldehyde, sectioned and stained with hematoxylin and eosin. Whole slide images were scanned and ten 400x images were randomly chosen throughout the tissue for further analysis. Using ImageJ software, alveolar wall width was measured, nuclei were counted and airspace was quantified. Morphological changes were identified in mice instilled with bleomycin. This combination offers a robust measure of lung morphology especially in a heterogenous injury.
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spelling pubmed-83653142021-08-23 Comprehensive dataset to assess morphological changes subsequent to bleomycin exposure Golden, Thea Murray, Alexa Venosa, Alessandro Gow, Andrew J. Data Brief Data Article Intratracheal bleomycin causes pulmonary injury, inflammation and fibrosis. The characteristic patchy nature of the injury makes analysis challenging. Histological assessment of lung injury is a useful tool to evaluate damage, however quantification is not standardized. We propose a multi-factorial approach to assess morphological changes subsequent to intratracheal bleomycin mediated lung injury. Lungs were inflation fixed with paraformaldehyde, sectioned and stained with hematoxylin and eosin. Whole slide images were scanned and ten 400x images were randomly chosen throughout the tissue for further analysis. Using ImageJ software, alveolar wall width was measured, nuclei were counted and airspace was quantified. Morphological changes were identified in mice instilled with bleomycin. This combination offers a robust measure of lung morphology especially in a heterogenous injury. Elsevier 2021-08-05 /pmc/articles/PMC8365314/ /pubmed/34430679 http://dx.doi.org/10.1016/j.dib.2021.107270 Text en © 2021 The Authors. Published by Elsevier Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Data Article
Golden, Thea
Murray, Alexa
Venosa, Alessandro
Gow, Andrew J.
Comprehensive dataset to assess morphological changes subsequent to bleomycin exposure
title Comprehensive dataset to assess morphological changes subsequent to bleomycin exposure
title_full Comprehensive dataset to assess morphological changes subsequent to bleomycin exposure
title_fullStr Comprehensive dataset to assess morphological changes subsequent to bleomycin exposure
title_full_unstemmed Comprehensive dataset to assess morphological changes subsequent to bleomycin exposure
title_short Comprehensive dataset to assess morphological changes subsequent to bleomycin exposure
title_sort comprehensive dataset to assess morphological changes subsequent to bleomycin exposure
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365314/
https://www.ncbi.nlm.nih.gov/pubmed/34430679
http://dx.doi.org/10.1016/j.dib.2021.107270
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