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A CRISPR/Cas9-based single-stranded DNA recombineering system for genome editing of Rhodococcus opacus PD630

Genome engineering of Rhodococcus opacus PD630, an important microorganism used for the bioconversion of lignin, is currently dependent on inefficient homologous recombination. Although a CRISPR interference procedure for gene repression has previously been developed for R. opacus PD630, a CRISPR/Ca...

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Detalles Bibliográficos
Autores principales:	Liang, Youxiang, Wei, Yuwen, Jiao, Song, Yu, Huimin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	KeAi Publishing 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365321/ https://www.ncbi.nlm.nih.gov/pubmed/34430726 http://dx.doi.org/10.1016/j.synbio.2021.08.001

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