Cargando…

Lung Inflation With Hydrogen During the Cold Ischemia Phase Alleviates Lung Ischemia-Reperfusion Injury by Inhibiting Pyroptosis in Rats

Background: Lung inflation with hydrogen is an effective method to protect donor lungs from lung ischemia-reperfusion injury (IRI). This study aimed to examine the effect of lung inflation with 3% hydrogen during the cold ischemia phase on pyroptosis in lung grafts of rats. Methods: Adult male Wista...

Descripción completa

Detalles Bibliográficos
Autores principales: Zheng, Panpan, Kang, Jiyu, Xing, Entong, Zheng, Bin, Wang, Xueyao, Zhou, Huacheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365359/
https://www.ncbi.nlm.nih.gov/pubmed/34408660
http://dx.doi.org/10.3389/fphys.2021.699344
_version_ 1783738692255350784
author Zheng, Panpan
Kang, Jiyu
Xing, Entong
Zheng, Bin
Wang, Xueyao
Zhou, Huacheng
author_facet Zheng, Panpan
Kang, Jiyu
Xing, Entong
Zheng, Bin
Wang, Xueyao
Zhou, Huacheng
author_sort Zheng, Panpan
collection PubMed
description Background: Lung inflation with hydrogen is an effective method to protect donor lungs from lung ischemia-reperfusion injury (IRI). This study aimed to examine the effect of lung inflation with 3% hydrogen during the cold ischemia phase on pyroptosis in lung grafts of rats. Methods: Adult male Wistar rats were randomly divided into the sham group, the control group, the oxygen (O(2)) group, and the hydrogen (H(2)) group. The sham group underwent thoracotomy but no lung transplantation. In the control group, the donor lungs were deflated for 2 h. In the O(2) and H(2) groups, the donor lungs were inflated with 40% O(2) + 60% N(2) and 3% H(2) + 40% O(2) + 57% N(2), respectively, at 10 ml/kg, and the gas was replaced every 20 min during the cold ischemia phase for 2 h. Two hours after orthotopic lung transplantation, the recipients were euthanized. Results: Compared with the control group, the O(2) and H(2) groups improved oxygenation indices, decreases the inflammatory response and oxidative stress, reduced lung injury, and improved pressure-volume (P-V) curves. H(2) had a better protective effect than O(2). Furthermore, the levels of the pyroptosis-related proteins selective nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3), cysteinyl aspartate specific proteinase (caspase)-1 p20, and the N-terminal of gasdermin D (GSDMD-N) were decreased in the H(2) group. Conclusion: Lung inflation with 3% hydrogen during the cold ischemia phase inhibited the inflammatory response, oxidative stress, and pyroptosis and improved the function of the graft. Inhibiting reactive oxygen species (ROS) production may be the main mechanism of the antipyroptotic effect of hydrogen.
format Online
Article
Text
id pubmed-8365359
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-83653592021-08-17 Lung Inflation With Hydrogen During the Cold Ischemia Phase Alleviates Lung Ischemia-Reperfusion Injury by Inhibiting Pyroptosis in Rats Zheng, Panpan Kang, Jiyu Xing, Entong Zheng, Bin Wang, Xueyao Zhou, Huacheng Front Physiol Physiology Background: Lung inflation with hydrogen is an effective method to protect donor lungs from lung ischemia-reperfusion injury (IRI). This study aimed to examine the effect of lung inflation with 3% hydrogen during the cold ischemia phase on pyroptosis in lung grafts of rats. Methods: Adult male Wistar rats were randomly divided into the sham group, the control group, the oxygen (O(2)) group, and the hydrogen (H(2)) group. The sham group underwent thoracotomy but no lung transplantation. In the control group, the donor lungs were deflated for 2 h. In the O(2) and H(2) groups, the donor lungs were inflated with 40% O(2) + 60% N(2) and 3% H(2) + 40% O(2) + 57% N(2), respectively, at 10 ml/kg, and the gas was replaced every 20 min during the cold ischemia phase for 2 h. Two hours after orthotopic lung transplantation, the recipients were euthanized. Results: Compared with the control group, the O(2) and H(2) groups improved oxygenation indices, decreases the inflammatory response and oxidative stress, reduced lung injury, and improved pressure-volume (P-V) curves. H(2) had a better protective effect than O(2). Furthermore, the levels of the pyroptosis-related proteins selective nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3), cysteinyl aspartate specific proteinase (caspase)-1 p20, and the N-terminal of gasdermin D (GSDMD-N) were decreased in the H(2) group. Conclusion: Lung inflation with 3% hydrogen during the cold ischemia phase inhibited the inflammatory response, oxidative stress, and pyroptosis and improved the function of the graft. Inhibiting reactive oxygen species (ROS) production may be the main mechanism of the antipyroptotic effect of hydrogen. Frontiers Media S.A. 2021-08-02 /pmc/articles/PMC8365359/ /pubmed/34408660 http://dx.doi.org/10.3389/fphys.2021.699344 Text en Copyright © 2021 Zheng, Kang, Xing, Zheng, Wang and Zhou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Zheng, Panpan
Kang, Jiyu
Xing, Entong
Zheng, Bin
Wang, Xueyao
Zhou, Huacheng
Lung Inflation With Hydrogen During the Cold Ischemia Phase Alleviates Lung Ischemia-Reperfusion Injury by Inhibiting Pyroptosis in Rats
title Lung Inflation With Hydrogen During the Cold Ischemia Phase Alleviates Lung Ischemia-Reperfusion Injury by Inhibiting Pyroptosis in Rats
title_full Lung Inflation With Hydrogen During the Cold Ischemia Phase Alleviates Lung Ischemia-Reperfusion Injury by Inhibiting Pyroptosis in Rats
title_fullStr Lung Inflation With Hydrogen During the Cold Ischemia Phase Alleviates Lung Ischemia-Reperfusion Injury by Inhibiting Pyroptosis in Rats
title_full_unstemmed Lung Inflation With Hydrogen During the Cold Ischemia Phase Alleviates Lung Ischemia-Reperfusion Injury by Inhibiting Pyroptosis in Rats
title_short Lung Inflation With Hydrogen During the Cold Ischemia Phase Alleviates Lung Ischemia-Reperfusion Injury by Inhibiting Pyroptosis in Rats
title_sort lung inflation with hydrogen during the cold ischemia phase alleviates lung ischemia-reperfusion injury by inhibiting pyroptosis in rats
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365359/
https://www.ncbi.nlm.nih.gov/pubmed/34408660
http://dx.doi.org/10.3389/fphys.2021.699344
work_keys_str_mv AT zhengpanpan lunginflationwithhydrogenduringthecoldischemiaphasealleviateslungischemiareperfusioninjurybyinhibitingpyroptosisinrats
AT kangjiyu lunginflationwithhydrogenduringthecoldischemiaphasealleviateslungischemiareperfusioninjurybyinhibitingpyroptosisinrats
AT xingentong lunginflationwithhydrogenduringthecoldischemiaphasealleviateslungischemiareperfusioninjurybyinhibitingpyroptosisinrats
AT zhengbin lunginflationwithhydrogenduringthecoldischemiaphasealleviateslungischemiareperfusioninjurybyinhibitingpyroptosisinrats
AT wangxueyao lunginflationwithhydrogenduringthecoldischemiaphasealleviateslungischemiareperfusioninjurybyinhibitingpyroptosisinrats
AT zhouhuacheng lunginflationwithhydrogenduringthecoldischemiaphasealleviateslungischemiareperfusioninjurybyinhibitingpyroptosisinrats