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A zygote-based assay to evaluate intranuclear shuttling in S. cerevisiae
It is often necessary to learn whether macromolecules occupy a fixed place in cells. This protocol makes it possible to learn whether individual nucleolar proteins in S. cerevisiae remain in place or depart from and return to the nucleolus. The protocol uses early zygotes in which parental nucleoli...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365525/ https://www.ncbi.nlm.nih.gov/pubmed/34430911 http://dx.doi.org/10.1016/j.xpro.2021.100736 |