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Rice aquaporin OsPIP2;2 is a water‐transporting facilitator in relevance to drought‐tolerant responses
In rice (Oryza sativa), the PLASMA MEMBRANE INTRINSIC PROTEIN (PIP) family of aquaporin has 11 members, OsPIP1;1 to OsPIP1;3, and OsPIP2;1 to OsPIP2;8, which are hypothesized to facilitate the transport of H(2)O and other small compounds across cell membranes. To date, however, only OsPIP1;2, OsPIP2...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365552/ https://www.ncbi.nlm.nih.gov/pubmed/34430793 http://dx.doi.org/10.1002/pld3.338 |
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author | Bai, Jiaqi Wang, Xuan Yao, Xiaohui Chen, Xiaochen Lu, Kai Hu, Yiqun Wang, Zuodong Mu, Yanjie Zhang, Liyuan Dong, Hansong |
author_facet | Bai, Jiaqi Wang, Xuan Yao, Xiaohui Chen, Xiaochen Lu, Kai Hu, Yiqun Wang, Zuodong Mu, Yanjie Zhang, Liyuan Dong, Hansong |
author_sort | Bai, Jiaqi |
collection | PubMed |
description | In rice (Oryza sativa), the PLASMA MEMBRANE INTRINSIC PROTEIN (PIP) family of aquaporin has 11 members, OsPIP1;1 to OsPIP1;3, and OsPIP2;1 to OsPIP2;8, which are hypothesized to facilitate the transport of H(2)O and other small compounds across cell membranes. To date, however, only OsPIP1;2, OsPIP2;1, and OsPIP2;4 have been demonstrated for substrate selectivity in their source plant (rice). In this study, OsPIP2;2 was characterized as the most efficient facilitator of H(2)O transport across cell membranes in comparison with the other 10 OsPIPs. In concomitant tests of all OsPIPs, four genes (OsPIP1;3, OsPIP2;1, OsPIP2;2, and OsPIP2;4) were induced to express in leaves of rice plants following a physiological drought stress, while OsPIP2;2 was expressed to the highest level. After de novo expression in frog oocytes and yeast cells, the four OsPIP proteins were localized to the plasma membranes in trimer and tetramer and displayed the activity to increase the membrane permeability to H(2)O. In comparison, OsPIP2;2 was most supportive to H(2)O import to oocytes and yeast cells. After de novo expression in tobacco protoplasts, OsPIP2;2 exceeded OsPIP1;3, OsPIP2;1, and OsPIP2;4 to support H(2)O transport across the plasma membranes. OsPIP2;2‐mediated H(2)O transport was accompanied by drought‐tolerant responses, including increases in concentrations of proline and polyamines, both of which are physiological markers of drought tolerance. In rice protoplasts, H(2)O transport and drought‐tolerant responses, which included expression of marker genes of drought tolerance pathway, were considerably enhanced by OsPIP2;2 overexpression but strongly inhibited by the gene silencing. Furthermore, OsPIP2;2 played a role in maintenance of the cell membrane integrity and effectively protected rice cells from electrolyte leakage caused by the physiological drought stress. These results suggest that OsPIP2;2 is a predominant facilitator of H(2)O transport in relevance to drought tolerance in the plant. |
format | Online Article Text |
id | pubmed-8365552 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83655522021-08-23 Rice aquaporin OsPIP2;2 is a water‐transporting facilitator in relevance to drought‐tolerant responses Bai, Jiaqi Wang, Xuan Yao, Xiaohui Chen, Xiaochen Lu, Kai Hu, Yiqun Wang, Zuodong Mu, Yanjie Zhang, Liyuan Dong, Hansong Plant Direct Original Research In rice (Oryza sativa), the PLASMA MEMBRANE INTRINSIC PROTEIN (PIP) family of aquaporin has 11 members, OsPIP1;1 to OsPIP1;3, and OsPIP2;1 to OsPIP2;8, which are hypothesized to facilitate the transport of H(2)O and other small compounds across cell membranes. To date, however, only OsPIP1;2, OsPIP2;1, and OsPIP2;4 have been demonstrated for substrate selectivity in their source plant (rice). In this study, OsPIP2;2 was characterized as the most efficient facilitator of H(2)O transport across cell membranes in comparison with the other 10 OsPIPs. In concomitant tests of all OsPIPs, four genes (OsPIP1;3, OsPIP2;1, OsPIP2;2, and OsPIP2;4) were induced to express in leaves of rice plants following a physiological drought stress, while OsPIP2;2 was expressed to the highest level. After de novo expression in frog oocytes and yeast cells, the four OsPIP proteins were localized to the plasma membranes in trimer and tetramer and displayed the activity to increase the membrane permeability to H(2)O. In comparison, OsPIP2;2 was most supportive to H(2)O import to oocytes and yeast cells. After de novo expression in tobacco protoplasts, OsPIP2;2 exceeded OsPIP1;3, OsPIP2;1, and OsPIP2;4 to support H(2)O transport across the plasma membranes. OsPIP2;2‐mediated H(2)O transport was accompanied by drought‐tolerant responses, including increases in concentrations of proline and polyamines, both of which are physiological markers of drought tolerance. In rice protoplasts, H(2)O transport and drought‐tolerant responses, which included expression of marker genes of drought tolerance pathway, were considerably enhanced by OsPIP2;2 overexpression but strongly inhibited by the gene silencing. Furthermore, OsPIP2;2 played a role in maintenance of the cell membrane integrity and effectively protected rice cells from electrolyte leakage caused by the physiological drought stress. These results suggest that OsPIP2;2 is a predominant facilitator of H(2)O transport in relevance to drought tolerance in the plant. John Wiley and Sons Inc. 2021-08-16 /pmc/articles/PMC8365552/ /pubmed/34430793 http://dx.doi.org/10.1002/pld3.338 Text en © 2021 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Bai, Jiaqi Wang, Xuan Yao, Xiaohui Chen, Xiaochen Lu, Kai Hu, Yiqun Wang, Zuodong Mu, Yanjie Zhang, Liyuan Dong, Hansong Rice aquaporin OsPIP2;2 is a water‐transporting facilitator in relevance to drought‐tolerant responses |
title | Rice aquaporin OsPIP2;2 is a water‐transporting facilitator in relevance to drought‐tolerant responses |
title_full | Rice aquaporin OsPIP2;2 is a water‐transporting facilitator in relevance to drought‐tolerant responses |
title_fullStr | Rice aquaporin OsPIP2;2 is a water‐transporting facilitator in relevance to drought‐tolerant responses |
title_full_unstemmed | Rice aquaporin OsPIP2;2 is a water‐transporting facilitator in relevance to drought‐tolerant responses |
title_short | Rice aquaporin OsPIP2;2 is a water‐transporting facilitator in relevance to drought‐tolerant responses |
title_sort | rice aquaporin ospip2;2 is a water‐transporting facilitator in relevance to drought‐tolerant responses |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365552/ https://www.ncbi.nlm.nih.gov/pubmed/34430793 http://dx.doi.org/10.1002/pld3.338 |
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