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Adenovirus is prevalent in juvenile polyps and correlates with low vitamin D receptor expression

BACKGROUND: The objective of this study was to assess human adenovirus (HAdV) infection in juvenile polyps (JPs) and to preliminarily establish a correlation to vitamin D receptor (VDR) expression. METHODS: The study includes 76 patients of 5.2 ± 2.8 years old. Seventy-eight JP specimens and 24 para...

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Autores principales: Wang, Lingling, Guo, Hongmei, Li, Jingwen, He, Susu, Yang, Guang, Li, Erguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365564/
https://www.ncbi.nlm.nih.gov/pubmed/34400787
http://dx.doi.org/10.1038/s41390-021-01697-y
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author Wang, Lingling
Guo, Hongmei
Li, Jingwen
He, Susu
Yang, Guang
Li, Erguang
author_facet Wang, Lingling
Guo, Hongmei
Li, Jingwen
He, Susu
Yang, Guang
Li, Erguang
author_sort Wang, Lingling
collection PubMed
description BACKGROUND: The objective of this study was to assess human adenovirus (HAdV) infection in juvenile polyps (JPs) and to preliminarily establish a correlation to vitamin D receptor (VDR) expression. METHODS: The study includes 76 patients of 5.2 ± 2.8 years old. Seventy-eight JP specimens and 24 parapolyp tissues from polypectomy were used. PCR was used to detect HAdV DNA and quantitative reverse transcription-PCR for viral and host gene expression. The PCR products were sequenced for virus typing. The correlation between VDR expression and HAdV infection was established using nonparametric Spearman’s analysis. RESULTS: Seventy-four children (97.4%) had a single polyp and two had two polyps. The histopathological characteristics of the polyps were in line with JP. Thirty-three samples had HAdV DNA (43.4%), including 32 subgroup C and 1 subgroup B HAdV; no enteric HAdV was detected. HAdV messenger RNA was detected in 5 of the 33 samples (15.2%). The samples had increased interleukin-1β (IL-1β), IL-6, and calprotectin expression, and reduced E-cadherin and VDR expression. JP samples with low VDR expression were more prevalent of HAdV DNA (r = 1.261, 95% confidence interval, 1.017–1.563), while VDR expression positively correlated with E-cadherin and negatively with inflammation gene expression. CONCLUSIONS: HAdV latent infection was prevalent among JP tissues. The presence of HAdV correlated positively to low VDR expression. IMPACT: The HAdVs infect the upper airways and gastrointestinal system and is found to persist in lymphoid tissues. The prevalence of HAdV and the status of the infection is unknown. The study investigated the prevalence of HAdV from polypectomy specimens of JP patients and found that HAdV was prevalent and was in a persistent state. HAdV infection was more prevalent in samples with low VDR expression. Whether HAdV infection and reactivation is a contributing factor to JPs is unknown. Factors such as proinflammation and bacterial metabolites that are known to promote HAdV reactivation warrant further investigation.
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spelling pubmed-83655642021-08-16 Adenovirus is prevalent in juvenile polyps and correlates with low vitamin D receptor expression Wang, Lingling Guo, Hongmei Li, Jingwen He, Susu Yang, Guang Li, Erguang Pediatr Res Basic Science Article BACKGROUND: The objective of this study was to assess human adenovirus (HAdV) infection in juvenile polyps (JPs) and to preliminarily establish a correlation to vitamin D receptor (VDR) expression. METHODS: The study includes 76 patients of 5.2 ± 2.8 years old. Seventy-eight JP specimens and 24 parapolyp tissues from polypectomy were used. PCR was used to detect HAdV DNA and quantitative reverse transcription-PCR for viral and host gene expression. The PCR products were sequenced for virus typing. The correlation between VDR expression and HAdV infection was established using nonparametric Spearman’s analysis. RESULTS: Seventy-four children (97.4%) had a single polyp and two had two polyps. The histopathological characteristics of the polyps were in line with JP. Thirty-three samples had HAdV DNA (43.4%), including 32 subgroup C and 1 subgroup B HAdV; no enteric HAdV was detected. HAdV messenger RNA was detected in 5 of the 33 samples (15.2%). The samples had increased interleukin-1β (IL-1β), IL-6, and calprotectin expression, and reduced E-cadherin and VDR expression. JP samples with low VDR expression were more prevalent of HAdV DNA (r = 1.261, 95% confidence interval, 1.017–1.563), while VDR expression positively correlated with E-cadherin and negatively with inflammation gene expression. CONCLUSIONS: HAdV latent infection was prevalent among JP tissues. The presence of HAdV correlated positively to low VDR expression. IMPACT: The HAdVs infect the upper airways and gastrointestinal system and is found to persist in lymphoid tissues. The prevalence of HAdV and the status of the infection is unknown. The study investigated the prevalence of HAdV from polypectomy specimens of JP patients and found that HAdV was prevalent and was in a persistent state. HAdV infection was more prevalent in samples with low VDR expression. Whether HAdV infection and reactivation is a contributing factor to JPs is unknown. Factors such as proinflammation and bacterial metabolites that are known to promote HAdV reactivation warrant further investigation. Nature Publishing Group US 2021-08-16 2022 /pmc/articles/PMC8365564/ /pubmed/34400787 http://dx.doi.org/10.1038/s41390-021-01697-y Text en © The Author(s), under exclusive licence to the International Pediatric Research Foundation, Inc 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Basic Science Article
Wang, Lingling
Guo, Hongmei
Li, Jingwen
He, Susu
Yang, Guang
Li, Erguang
Adenovirus is prevalent in juvenile polyps and correlates with low vitamin D receptor expression
title Adenovirus is prevalent in juvenile polyps and correlates with low vitamin D receptor expression
title_full Adenovirus is prevalent in juvenile polyps and correlates with low vitamin D receptor expression
title_fullStr Adenovirus is prevalent in juvenile polyps and correlates with low vitamin D receptor expression
title_full_unstemmed Adenovirus is prevalent in juvenile polyps and correlates with low vitamin D receptor expression
title_short Adenovirus is prevalent in juvenile polyps and correlates with low vitamin D receptor expression
title_sort adenovirus is prevalent in juvenile polyps and correlates with low vitamin d receptor expression
topic Basic Science Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365564/
https://www.ncbi.nlm.nih.gov/pubmed/34400787
http://dx.doi.org/10.1038/s41390-021-01697-y
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