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Biological Characterization and Evolution of Bacteriophage T7-△holin During the Serial Passage Process

Bacteriophage T7 gene 17.5 coding for the only known holin is one of the components of its lysis system, but the holin activity in T7 is more complex than a single gene, and evidence points to the existence of additional T7 genes with holin activity. In this study, a T7 phage with a gene 17.5 deleti...

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Autores principales: Xu, Hai, Bao, Xi, Hong, Weiming, Wang, Anping, Wang, Kaimin, Dong, Hongyan, Hou, Jibo, Govinden, Roshini, Deng, Bihua, Chenia, Hafizah Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365609/
https://www.ncbi.nlm.nih.gov/pubmed/34408735
http://dx.doi.org/10.3389/fmicb.2021.705310
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author Xu, Hai
Bao, Xi
Hong, Weiming
Wang, Anping
Wang, Kaimin
Dong, Hongyan
Hou, Jibo
Govinden, Roshini
Deng, Bihua
Chenia, Hafizah Y.
author_facet Xu, Hai
Bao, Xi
Hong, Weiming
Wang, Anping
Wang, Kaimin
Dong, Hongyan
Hou, Jibo
Govinden, Roshini
Deng, Bihua
Chenia, Hafizah Y.
author_sort Xu, Hai
collection PubMed
description Bacteriophage T7 gene 17.5 coding for the only known holin is one of the components of its lysis system, but the holin activity in T7 is more complex than a single gene, and evidence points to the existence of additional T7 genes with holin activity. In this study, a T7 phage with a gene 17.5 deletion (T7-△holin) was rescued and its biological characteristics and effect on cell lysis were determined. Furthermore, the genomic evolution of mutant phage T7-△holin during serial passage was assessed by whole-genome sequencing analysis. It was observed that deletion of gene 17.5 from phage T7 delays lysis time and enlarges the phage burst size; however, this biological characteristic recovered to normal lysis levels during serial passage. Scanning electron microscopy showed that the two opposite ends of E. coli BL21 cells swell post-T7-△holin infection rather than drilling holes on cell membrane when compared with T7 wild-type infection. No visible progeny phage particle accumulation was observed inside the E. coli BL21 cells by transmission electron microscopy. Following serial passage of T7-△holin from the 1st to 20th generations, the mRNA levels of gene 3.5 and gene 19.5 were upregulated and several mutation sites were discovered, especially two missense mutations in gene 19.5, which indicate a potential contribution to the evolution of the T7-△holin. Although the burst size of T7-△holin increased, high titer cultivation of T7-△holin was not achieved by optimizing the culture process. Accordingly, these results suggest that gene 19.5 is a potential lysis-related component that needs to be studied further and that the T7-△holin strain with its gene 17.5 deletion is not adequate to establish the high-titer phage cultivation process.
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spelling pubmed-83656092021-08-17 Biological Characterization and Evolution of Bacteriophage T7-△holin During the Serial Passage Process Xu, Hai Bao, Xi Hong, Weiming Wang, Anping Wang, Kaimin Dong, Hongyan Hou, Jibo Govinden, Roshini Deng, Bihua Chenia, Hafizah Y. Front Microbiol Microbiology Bacteriophage T7 gene 17.5 coding for the only known holin is one of the components of its lysis system, but the holin activity in T7 is more complex than a single gene, and evidence points to the existence of additional T7 genes with holin activity. In this study, a T7 phage with a gene 17.5 deletion (T7-△holin) was rescued and its biological characteristics and effect on cell lysis were determined. Furthermore, the genomic evolution of mutant phage T7-△holin during serial passage was assessed by whole-genome sequencing analysis. It was observed that deletion of gene 17.5 from phage T7 delays lysis time and enlarges the phage burst size; however, this biological characteristic recovered to normal lysis levels during serial passage. Scanning electron microscopy showed that the two opposite ends of E. coli BL21 cells swell post-T7-△holin infection rather than drilling holes on cell membrane when compared with T7 wild-type infection. No visible progeny phage particle accumulation was observed inside the E. coli BL21 cells by transmission electron microscopy. Following serial passage of T7-△holin from the 1st to 20th generations, the mRNA levels of gene 3.5 and gene 19.5 were upregulated and several mutation sites were discovered, especially two missense mutations in gene 19.5, which indicate a potential contribution to the evolution of the T7-△holin. Although the burst size of T7-△holin increased, high titer cultivation of T7-△holin was not achieved by optimizing the culture process. Accordingly, these results suggest that gene 19.5 is a potential lysis-related component that needs to be studied further and that the T7-△holin strain with its gene 17.5 deletion is not adequate to establish the high-titer phage cultivation process. Frontiers Media S.A. 2021-08-02 /pmc/articles/PMC8365609/ /pubmed/34408735 http://dx.doi.org/10.3389/fmicb.2021.705310 Text en Copyright © 2021 Xu, Bao, Hong, Wang, Wang, Dong, Hou, Govinden, Deng and Chenia. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Xu, Hai
Bao, Xi
Hong, Weiming
Wang, Anping
Wang, Kaimin
Dong, Hongyan
Hou, Jibo
Govinden, Roshini
Deng, Bihua
Chenia, Hafizah Y.
Biological Characterization and Evolution of Bacteriophage T7-△holin During the Serial Passage Process
title Biological Characterization and Evolution of Bacteriophage T7-△holin During the Serial Passage Process
title_full Biological Characterization and Evolution of Bacteriophage T7-△holin During the Serial Passage Process
title_fullStr Biological Characterization and Evolution of Bacteriophage T7-△holin During the Serial Passage Process
title_full_unstemmed Biological Characterization and Evolution of Bacteriophage T7-△holin During the Serial Passage Process
title_short Biological Characterization and Evolution of Bacteriophage T7-△holin During the Serial Passage Process
title_sort biological characterization and evolution of bacteriophage t7-△holin during the serial passage process
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365609/
https://www.ncbi.nlm.nih.gov/pubmed/34408735
http://dx.doi.org/10.3389/fmicb.2021.705310
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