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Regulator of Chromosome Condensation 1-Domain Protein DEK47 Functions on the Intron Splicing of Mitochondrial Nad2 and Seed Development in Maize

In flowering plants, mitochondrial genes contain approximately 20–26 introns. Splicing of these introns is essential for mitochondrial gene expression and function. Recent studies have revealed that both nucleus- and mitochondrion-encoded factors are required for intron splicing, but the mechanism o...

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Autores principales: Cao, Shi-Kai, Liu, Rui, Sayyed, Aqib, Sun, Feng, Song, Ruolin, Wang, Xiaomin, Xiu, Zhihui, Li, Xiaojie, Tan, Bao-Cai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365749/
https://www.ncbi.nlm.nih.gov/pubmed/34408760
http://dx.doi.org/10.3389/fpls.2021.695249
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author Cao, Shi-Kai
Liu, Rui
Sayyed, Aqib
Sun, Feng
Song, Ruolin
Wang, Xiaomin
Xiu, Zhihui
Li, Xiaojie
Tan, Bao-Cai
author_facet Cao, Shi-Kai
Liu, Rui
Sayyed, Aqib
Sun, Feng
Song, Ruolin
Wang, Xiaomin
Xiu, Zhihui
Li, Xiaojie
Tan, Bao-Cai
author_sort Cao, Shi-Kai
collection PubMed
description In flowering plants, mitochondrial genes contain approximately 20–26 introns. Splicing of these introns is essential for mitochondrial gene expression and function. Recent studies have revealed that both nucleus- and mitochondrion-encoded factors are required for intron splicing, but the mechanism of splicing remains largely unknown. Elucidation of the mechanism necessitates a complete understanding of the splicing factors. Here, we report the identification of a regulator of chromosome condensation 1 (RCC1)-domain protein DEK47 that is required for mitochondrial intron splicing and seed development in maize. Loss of function in Dek47 severely arrests embryo and endosperm development, resulting in a defective kernel (dek) phenotype. DEK47 harbors seven RCC1 domains and is targeted to mitochondria. Null mutation of DEK47 causes a deficiency in the splicing of all four nad2 introns, abolishing the production of mature nad2 transcript and resulting in the disassembly and severely reduced activity of mitochondrial complex I. In response, the expression of the alternative oxidase AOX2 is sharply increased in dek47. These results indicate that Dek47 is required for the splicing of all the nad2 introns in mitochondria, and essential for complex I assembly, and kernel development in maize.
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spelling pubmed-83657492021-08-17 Regulator of Chromosome Condensation 1-Domain Protein DEK47 Functions on the Intron Splicing of Mitochondrial Nad2 and Seed Development in Maize Cao, Shi-Kai Liu, Rui Sayyed, Aqib Sun, Feng Song, Ruolin Wang, Xiaomin Xiu, Zhihui Li, Xiaojie Tan, Bao-Cai Front Plant Sci Plant Science In flowering plants, mitochondrial genes contain approximately 20–26 introns. Splicing of these introns is essential for mitochondrial gene expression and function. Recent studies have revealed that both nucleus- and mitochondrion-encoded factors are required for intron splicing, but the mechanism of splicing remains largely unknown. Elucidation of the mechanism necessitates a complete understanding of the splicing factors. Here, we report the identification of a regulator of chromosome condensation 1 (RCC1)-domain protein DEK47 that is required for mitochondrial intron splicing and seed development in maize. Loss of function in Dek47 severely arrests embryo and endosperm development, resulting in a defective kernel (dek) phenotype. DEK47 harbors seven RCC1 domains and is targeted to mitochondria. Null mutation of DEK47 causes a deficiency in the splicing of all four nad2 introns, abolishing the production of mature nad2 transcript and resulting in the disassembly and severely reduced activity of mitochondrial complex I. In response, the expression of the alternative oxidase AOX2 is sharply increased in dek47. These results indicate that Dek47 is required for the splicing of all the nad2 introns in mitochondria, and essential for complex I assembly, and kernel development in maize. Frontiers Media S.A. 2021-08-02 /pmc/articles/PMC8365749/ /pubmed/34408760 http://dx.doi.org/10.3389/fpls.2021.695249 Text en Copyright © 2021 Cao, Liu, Sayyed, Sun, Song, Wang, Xiu, Li and Tan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Cao, Shi-Kai
Liu, Rui
Sayyed, Aqib
Sun, Feng
Song, Ruolin
Wang, Xiaomin
Xiu, Zhihui
Li, Xiaojie
Tan, Bao-Cai
Regulator of Chromosome Condensation 1-Domain Protein DEK47 Functions on the Intron Splicing of Mitochondrial Nad2 and Seed Development in Maize
title Regulator of Chromosome Condensation 1-Domain Protein DEK47 Functions on the Intron Splicing of Mitochondrial Nad2 and Seed Development in Maize
title_full Regulator of Chromosome Condensation 1-Domain Protein DEK47 Functions on the Intron Splicing of Mitochondrial Nad2 and Seed Development in Maize
title_fullStr Regulator of Chromosome Condensation 1-Domain Protein DEK47 Functions on the Intron Splicing of Mitochondrial Nad2 and Seed Development in Maize
title_full_unstemmed Regulator of Chromosome Condensation 1-Domain Protein DEK47 Functions on the Intron Splicing of Mitochondrial Nad2 and Seed Development in Maize
title_short Regulator of Chromosome Condensation 1-Domain Protein DEK47 Functions on the Intron Splicing of Mitochondrial Nad2 and Seed Development in Maize
title_sort regulator of chromosome condensation 1-domain protein dek47 functions on the intron splicing of mitochondrial nad2 and seed development in maize
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365749/
https://www.ncbi.nlm.nih.gov/pubmed/34408760
http://dx.doi.org/10.3389/fpls.2021.695249
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