Cargando…

Human esophageal fibroblast-derived exosomal miR-21 reduced the cisplatin sensitivity to esophageal carcinoma EC9706 cells

The objective of this study was to investigate the effect of human esophageal fibroblast-derived exosomal miR-21 on cisplatin sensitivity against esophageal squamous EC9706 cells. EC9706 cells were co-cultured indirectly with human esophageal fibroblasts (HEF) or miR-21 mimics transfected-HEF in the...

Descripción completa

Detalles Bibliográficos
Autores principales: Wan, Jiajin, Niu, Chunling, Wang, Baiyan, Han, Qianqian, Chen, Yulon, Feng, Shuying, Yang, Lianhe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Associação Brasileira de Divulgação Científica 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365874/
https://www.ncbi.nlm.nih.gov/pubmed/34378676
http://dx.doi.org/10.1590/1414-431X2021e11156
_version_ 1783738798039891968
author Wan, Jiajin
Niu, Chunling
Wang, Baiyan
Han, Qianqian
Chen, Yulon
Feng, Shuying
Yang, Lianhe
author_facet Wan, Jiajin
Niu, Chunling
Wang, Baiyan
Han, Qianqian
Chen, Yulon
Feng, Shuying
Yang, Lianhe
author_sort Wan, Jiajin
collection PubMed
description The objective of this study was to investigate the effect of human esophageal fibroblast-derived exosomal miR-21 on cisplatin sensitivity against esophageal squamous EC9706 cells. EC9706 cells were co-cultured indirectly with human esophageal fibroblasts (HEF) or miR-21 mimics transfected-HEF in the transwell system. The exosomes in HEF-culture conditioned medium were extracted by differential ultracentrifugation. EC9706 cells were co-cultured with HEF-derived exosomes directly. The cisplatin sensitivity against EC9706 cells was revealed via half maximal inhibitory concentration (IC(50)) values using MTT assay. The expressions of miR-21, programmed cell death 4 (PDCD4) mRNA, and gene of phosphate and tension homology deleted on chromosome ten (PTEN) mRNA were determined by qRT-PCR. The changes of the protein level were detected using western blot assay. IC(50) values of cisplatin against EC9706 cells were increased after EC9706 cells were co-cultured with either HEF or exosomes derived from miR-21 mimics-transfected HEF. Following the increased level of miR-21, the mRNA expression and protein levels of PTEN and PDCD4 were decreased in EC9706 cells. The cisplatin sensitivity to EC9706 cells was reduced by HEF-derived exosomal miR-21 through targeting PTEN and PDCD4. This study suggested that non-tumor cells in the tumor micro-environment increased the tumor anti-chemotherapy effects through their exosomes.
format Online
Article
Text
id pubmed-8365874
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Associação Brasileira de Divulgação Científica
record_format MEDLINE/PubMed
spelling pubmed-83658742021-08-26 Human esophageal fibroblast-derived exosomal miR-21 reduced the cisplatin sensitivity to esophageal carcinoma EC9706 cells Wan, Jiajin Niu, Chunling Wang, Baiyan Han, Qianqian Chen, Yulon Feng, Shuying Yang, Lianhe Braz J Med Biol Res Research Article The objective of this study was to investigate the effect of human esophageal fibroblast-derived exosomal miR-21 on cisplatin sensitivity against esophageal squamous EC9706 cells. EC9706 cells were co-cultured indirectly with human esophageal fibroblasts (HEF) or miR-21 mimics transfected-HEF in the transwell system. The exosomes in HEF-culture conditioned medium were extracted by differential ultracentrifugation. EC9706 cells were co-cultured with HEF-derived exosomes directly. The cisplatin sensitivity against EC9706 cells was revealed via half maximal inhibitory concentration (IC(50)) values using MTT assay. The expressions of miR-21, programmed cell death 4 (PDCD4) mRNA, and gene of phosphate and tension homology deleted on chromosome ten (PTEN) mRNA were determined by qRT-PCR. The changes of the protein level were detected using western blot assay. IC(50) values of cisplatin against EC9706 cells were increased after EC9706 cells were co-cultured with either HEF or exosomes derived from miR-21 mimics-transfected HEF. Following the increased level of miR-21, the mRNA expression and protein levels of PTEN and PDCD4 were decreased in EC9706 cells. The cisplatin sensitivity to EC9706 cells was reduced by HEF-derived exosomal miR-21 through targeting PTEN and PDCD4. This study suggested that non-tumor cells in the tumor micro-environment increased the tumor anti-chemotherapy effects through their exosomes. Associação Brasileira de Divulgação Científica 2021-08-06 /pmc/articles/PMC8365874/ /pubmed/34378676 http://dx.doi.org/10.1590/1414-431X2021e11156 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wan, Jiajin
Niu, Chunling
Wang, Baiyan
Han, Qianqian
Chen, Yulon
Feng, Shuying
Yang, Lianhe
Human esophageal fibroblast-derived exosomal miR-21 reduced the cisplatin sensitivity to esophageal carcinoma EC9706 cells
title Human esophageal fibroblast-derived exosomal miR-21 reduced the cisplatin sensitivity to esophageal carcinoma EC9706 cells
title_full Human esophageal fibroblast-derived exosomal miR-21 reduced the cisplatin sensitivity to esophageal carcinoma EC9706 cells
title_fullStr Human esophageal fibroblast-derived exosomal miR-21 reduced the cisplatin sensitivity to esophageal carcinoma EC9706 cells
title_full_unstemmed Human esophageal fibroblast-derived exosomal miR-21 reduced the cisplatin sensitivity to esophageal carcinoma EC9706 cells
title_short Human esophageal fibroblast-derived exosomal miR-21 reduced the cisplatin sensitivity to esophageal carcinoma EC9706 cells
title_sort human esophageal fibroblast-derived exosomal mir-21 reduced the cisplatin sensitivity to esophageal carcinoma ec9706 cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365874/
https://www.ncbi.nlm.nih.gov/pubmed/34378676
http://dx.doi.org/10.1590/1414-431X2021e11156
work_keys_str_mv AT wanjiajin humanesophagealfibroblastderivedexosomalmir21reducedthecisplatinsensitivitytoesophagealcarcinomaec9706cells
AT niuchunling humanesophagealfibroblastderivedexosomalmir21reducedthecisplatinsensitivitytoesophagealcarcinomaec9706cells
AT wangbaiyan humanesophagealfibroblastderivedexosomalmir21reducedthecisplatinsensitivitytoesophagealcarcinomaec9706cells
AT hanqianqian humanesophagealfibroblastderivedexosomalmir21reducedthecisplatinsensitivitytoesophagealcarcinomaec9706cells
AT chenyulon humanesophagealfibroblastderivedexosomalmir21reducedthecisplatinsensitivitytoesophagealcarcinomaec9706cells
AT fengshuying humanesophagealfibroblastderivedexosomalmir21reducedthecisplatinsensitivitytoesophagealcarcinomaec9706cells
AT yanglianhe humanesophagealfibroblastderivedexosomalmir21reducedthecisplatinsensitivitytoesophagealcarcinomaec9706cells