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Screening of miRNAs in plasma as a diagnostic biomarker for cardiac disease based on optimization of extraction and qRT-PCR condition assay through amplification efficiency

BACKGROUND: Although quantitative real-time PCR (qRT-PCR) is a common and sensitive method for miRNAs analysis, it is necessary to optimize conditions and minimize qRT-PCR inhibitors to achieve reliable results. The aim of this study was to minimize interference by contaminants in qRT-PCR, maximize...

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Detalles Bibliográficos
Autores principales:	Ban, Eunmi, Kwon, Haejin, Seo, Hong Seog, Yoo, Young Sook, Song, Eun Joo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2021
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8366012/ https://www.ncbi.nlm.nih.gov/pubmed/34399741 http://dx.doi.org/10.1186/s12896-021-00710-w

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