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Ultrastructural plasma membrane asymmetries in tension and curvature promote yeast cell fusion
Cell–cell fusion is central for sexual reproduction, and generally involves gametes of different shapes and sizes. In walled fission yeast Schizosaccharomyces pombe, the fusion of h+ and h− isogametes requires the fusion focus, an actin structure that concentrates glucanase-containing vesicles for c...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8366684/ https://www.ncbi.nlm.nih.gov/pubmed/34382996 http://dx.doi.org/10.1083/jcb.202103142 |
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author | Muriel, Olivia Michon, Laetitia Kukulski, Wanda Martin, Sophie G. |
author_facet | Muriel, Olivia Michon, Laetitia Kukulski, Wanda Martin, Sophie G. |
author_sort | Muriel, Olivia |
collection | PubMed |
description | Cell–cell fusion is central for sexual reproduction, and generally involves gametes of different shapes and sizes. In walled fission yeast Schizosaccharomyces pombe, the fusion of h+ and h− isogametes requires the fusion focus, an actin structure that concentrates glucanase-containing vesicles for cell wall digestion. Here, we present a quantitative correlative light and electron microscopy (CLEM) tomographic dataset of the fusion site, which reveals the fusion focus ultrastructure. Unexpectedly, gametes show marked asymmetries: a taut, convex plasma membrane of h− cells progressively protrudes into a more slack, wavy plasma membrane of h+ cells. Asymmetries are relaxed upon fusion, with observations of ramified fusion pores. h+ cells have a higher exo-/endocytosis ratio than h− cells, and local reduction in exocytosis strongly diminishes membrane waviness. Reciprocally, turgor pressure reduction specifically in h− cells impedes their protrusions into h+ cells and delays cell fusion. We hypothesize that asymmetric membrane conformations, due to differential turgor pressure and exocytosis/endocytosis ratios between mating types, favor cell–cell fusion. |
format | Online Article Text |
id | pubmed-8366684 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-83666842022-04-04 Ultrastructural plasma membrane asymmetries in tension and curvature promote yeast cell fusion Muriel, Olivia Michon, Laetitia Kukulski, Wanda Martin, Sophie G. J Cell Biol Article Cell–cell fusion is central for sexual reproduction, and generally involves gametes of different shapes and sizes. In walled fission yeast Schizosaccharomyces pombe, the fusion of h+ and h− isogametes requires the fusion focus, an actin structure that concentrates glucanase-containing vesicles for cell wall digestion. Here, we present a quantitative correlative light and electron microscopy (CLEM) tomographic dataset of the fusion site, which reveals the fusion focus ultrastructure. Unexpectedly, gametes show marked asymmetries: a taut, convex plasma membrane of h− cells progressively protrudes into a more slack, wavy plasma membrane of h+ cells. Asymmetries are relaxed upon fusion, with observations of ramified fusion pores. h+ cells have a higher exo-/endocytosis ratio than h− cells, and local reduction in exocytosis strongly diminishes membrane waviness. Reciprocally, turgor pressure reduction specifically in h− cells impedes their protrusions into h+ cells and delays cell fusion. We hypothesize that asymmetric membrane conformations, due to differential turgor pressure and exocytosis/endocytosis ratios between mating types, favor cell–cell fusion. Rockefeller University Press 2021-08-12 /pmc/articles/PMC8366684/ /pubmed/34382996 http://dx.doi.org/10.1083/jcb.202103142 Text en © 2021 Muriel et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Muriel, Olivia Michon, Laetitia Kukulski, Wanda Martin, Sophie G. Ultrastructural plasma membrane asymmetries in tension and curvature promote yeast cell fusion |
title | Ultrastructural plasma membrane asymmetries in tension and curvature promote yeast cell fusion |
title_full | Ultrastructural plasma membrane asymmetries in tension and curvature promote yeast cell fusion |
title_fullStr | Ultrastructural plasma membrane asymmetries in tension and curvature promote yeast cell fusion |
title_full_unstemmed | Ultrastructural plasma membrane asymmetries in tension and curvature promote yeast cell fusion |
title_short | Ultrastructural plasma membrane asymmetries in tension and curvature promote yeast cell fusion |
title_sort | ultrastructural plasma membrane asymmetries in tension and curvature promote yeast cell fusion |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8366684/ https://www.ncbi.nlm.nih.gov/pubmed/34382996 http://dx.doi.org/10.1083/jcb.202103142 |
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