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Active rheumatoid arthritis in a mouse model is not an independent risk factor for periprosthetic joint infection

INTRODUCTION: Periprosthetic joint infection (PJI) represents a devastating complication of total joint arthroplasty associated with significant morbidity and mortality. Literature suggests a possible higher incidence of periprosthetic joint infection (PJI) in patients with rheumatoid arthritis (RA)...

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Autores principales: Trikha, Rishi, Greig, Danielle, Sekimura, Troy, Cevallos, Nicolas, Kelley, Benjamin, Mamouei, Zeinab, Hart, Christopher, Ralston, Micah, Turkmani, Amr, Sassoon, Adam, Stavrakis, Alexandra, Bernthal, Nicholas M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8366981/
https://www.ncbi.nlm.nih.gov/pubmed/34398899
http://dx.doi.org/10.1371/journal.pone.0250910
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author Trikha, Rishi
Greig, Danielle
Sekimura, Troy
Cevallos, Nicolas
Kelley, Benjamin
Mamouei, Zeinab
Hart, Christopher
Ralston, Micah
Turkmani, Amr
Sassoon, Adam
Stavrakis, Alexandra
Bernthal, Nicholas M.
author_facet Trikha, Rishi
Greig, Danielle
Sekimura, Troy
Cevallos, Nicolas
Kelley, Benjamin
Mamouei, Zeinab
Hart, Christopher
Ralston, Micah
Turkmani, Amr
Sassoon, Adam
Stavrakis, Alexandra
Bernthal, Nicholas M.
author_sort Trikha, Rishi
collection PubMed
description INTRODUCTION: Periprosthetic joint infection (PJI) represents a devastating complication of total joint arthroplasty associated with significant morbidity and mortality. Literature suggests a possible higher incidence of periprosthetic joint infection (PJI) in patients with rheumatoid arthritis (RA). There is, however, no consensus on this purported risk nor a well-defined mechanism. This study investigates how collagen-induced arthritis (CIA), a validated animal model of RA, impacts infectious burden in a well-established model of PJI. METHODS: Control mice were compared against CIA mice. Whole blood samples were collected to quantify systemic IgG levels via ELISA. Ex vivo respiratory burst function was measured via dihydrorhodamine assay. Ex vivo Staphylococcus aureus Xen36 burden was measured directly via colony forming unit (CFU) counts and crystal violet assay to assess biofilm formation. In vivo, surgical placement of a titanium implant through the knee joint and inoculation with S. aureus Xen36 was performed. Bacterial burden was then quantified by longitudinal bioluminescent imaging. RESULTS: Mice with CIA demonstrated significantly higher levels of systemic IgG compared with control mice (p = 0.003). Ex vivo, there was no significant difference in respiratory burst function (p = 0.89) or S. aureus bacterial burden as measured by CFU counts (p = 0.91) and crystal violet assay (p = 0.96). In vivo, no significant difference in bacterial bioluminescence between groups was found at all postoperative time points. CFU counts of both the implant and the peri-implant tissue were not significantly different between groups (p = 0.82 and 0.80, respectively). CONCLUSION: This study demonstrated no significant difference in S. aureus infectious burden between mice with CIA and control mice. These results suggest that untreated, active RA may not represent a significant intrinsic risk factor for PJI, however further mechanistic translational and clinical studies are warranted.
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spelling pubmed-83669812021-08-17 Active rheumatoid arthritis in a mouse model is not an independent risk factor for periprosthetic joint infection Trikha, Rishi Greig, Danielle Sekimura, Troy Cevallos, Nicolas Kelley, Benjamin Mamouei, Zeinab Hart, Christopher Ralston, Micah Turkmani, Amr Sassoon, Adam Stavrakis, Alexandra Bernthal, Nicholas M. PLoS One Research Article INTRODUCTION: Periprosthetic joint infection (PJI) represents a devastating complication of total joint arthroplasty associated with significant morbidity and mortality. Literature suggests a possible higher incidence of periprosthetic joint infection (PJI) in patients with rheumatoid arthritis (RA). There is, however, no consensus on this purported risk nor a well-defined mechanism. This study investigates how collagen-induced arthritis (CIA), a validated animal model of RA, impacts infectious burden in a well-established model of PJI. METHODS: Control mice were compared against CIA mice. Whole blood samples were collected to quantify systemic IgG levels via ELISA. Ex vivo respiratory burst function was measured via dihydrorhodamine assay. Ex vivo Staphylococcus aureus Xen36 burden was measured directly via colony forming unit (CFU) counts and crystal violet assay to assess biofilm formation. In vivo, surgical placement of a titanium implant through the knee joint and inoculation with S. aureus Xen36 was performed. Bacterial burden was then quantified by longitudinal bioluminescent imaging. RESULTS: Mice with CIA demonstrated significantly higher levels of systemic IgG compared with control mice (p = 0.003). Ex vivo, there was no significant difference in respiratory burst function (p = 0.89) or S. aureus bacterial burden as measured by CFU counts (p = 0.91) and crystal violet assay (p = 0.96). In vivo, no significant difference in bacterial bioluminescence between groups was found at all postoperative time points. CFU counts of both the implant and the peri-implant tissue were not significantly different between groups (p = 0.82 and 0.80, respectively). CONCLUSION: This study demonstrated no significant difference in S. aureus infectious burden between mice with CIA and control mice. These results suggest that untreated, active RA may not represent a significant intrinsic risk factor for PJI, however further mechanistic translational and clinical studies are warranted. Public Library of Science 2021-08-16 /pmc/articles/PMC8366981/ /pubmed/34398899 http://dx.doi.org/10.1371/journal.pone.0250910 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Trikha, Rishi
Greig, Danielle
Sekimura, Troy
Cevallos, Nicolas
Kelley, Benjamin
Mamouei, Zeinab
Hart, Christopher
Ralston, Micah
Turkmani, Amr
Sassoon, Adam
Stavrakis, Alexandra
Bernthal, Nicholas M.
Active rheumatoid arthritis in a mouse model is not an independent risk factor for periprosthetic joint infection
title Active rheumatoid arthritis in a mouse model is not an independent risk factor for periprosthetic joint infection
title_full Active rheumatoid arthritis in a mouse model is not an independent risk factor for periprosthetic joint infection
title_fullStr Active rheumatoid arthritis in a mouse model is not an independent risk factor for periprosthetic joint infection
title_full_unstemmed Active rheumatoid arthritis in a mouse model is not an independent risk factor for periprosthetic joint infection
title_short Active rheumatoid arthritis in a mouse model is not an independent risk factor for periprosthetic joint infection
title_sort active rheumatoid arthritis in a mouse model is not an independent risk factor for periprosthetic joint infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8366981/
https://www.ncbi.nlm.nih.gov/pubmed/34398899
http://dx.doi.org/10.1371/journal.pone.0250910
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