Cargando…
Spatially patterned excitatory neuron subtypes and projections of the claustrum
The claustrum is a functionally and structurally complex brain region, whose very spatial extent remains debated. Histochemical-based approaches typically treat the claustrum as a relatively narrow anatomical region that primarily projects to the neocortex, whereas circuit-based approaches can sugge...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8367382/ https://www.ncbi.nlm.nih.gov/pubmed/34397382 http://dx.doi.org/10.7554/eLife.68967 |
_version_ | 1783739046067961856 |
---|---|
author | Erwin, Sarah R Bristow, Brianna N Sullivan, Kaitlin E Kendrick, Rennie M Marriott, Brian Wang, Lihua Clements, Jody Lemire, Andrew L Jackson, Jesse Cembrowski, Mark S |
author_facet | Erwin, Sarah R Bristow, Brianna N Sullivan, Kaitlin E Kendrick, Rennie M Marriott, Brian Wang, Lihua Clements, Jody Lemire, Andrew L Jackson, Jesse Cembrowski, Mark S |
author_sort | Erwin, Sarah R |
collection | PubMed |
description | The claustrum is a functionally and structurally complex brain region, whose very spatial extent remains debated. Histochemical-based approaches typically treat the claustrum as a relatively narrow anatomical region that primarily projects to the neocortex, whereas circuit-based approaches can suggest a broader claustrum region containing projections to the neocortex and other regions. Here, in the mouse, we took a bottom-up and cell-type-specific approach to complement and possibly unite these seemingly disparate conclusions. Using single-cell RNA-sequencing, we found that the claustrum comprises two excitatory neuron subtypes that are differentiable from the surrounding cortex. Multicolor retrograde tracing in conjunction with 12-channel multiplexed in situ hybridization revealed a core-shell spatial arrangement of these subtypes, as well as differential downstream targets. Thus, the claustrum comprises excitatory neuron subtypes with distinct molecular and projection properties, whose spatial patterns reflect the narrower and broader claustral extents debated in previous research. This subtype-specific heterogeneity likely shapes the functional complexity of the claustrum. |
format | Online Article Text |
id | pubmed-8367382 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-83673822021-08-18 Spatially patterned excitatory neuron subtypes and projections of the claustrum Erwin, Sarah R Bristow, Brianna N Sullivan, Kaitlin E Kendrick, Rennie M Marriott, Brian Wang, Lihua Clements, Jody Lemire, Andrew L Jackson, Jesse Cembrowski, Mark S eLife Neuroscience The claustrum is a functionally and structurally complex brain region, whose very spatial extent remains debated. Histochemical-based approaches typically treat the claustrum as a relatively narrow anatomical region that primarily projects to the neocortex, whereas circuit-based approaches can suggest a broader claustrum region containing projections to the neocortex and other regions. Here, in the mouse, we took a bottom-up and cell-type-specific approach to complement and possibly unite these seemingly disparate conclusions. Using single-cell RNA-sequencing, we found that the claustrum comprises two excitatory neuron subtypes that are differentiable from the surrounding cortex. Multicolor retrograde tracing in conjunction with 12-channel multiplexed in situ hybridization revealed a core-shell spatial arrangement of these subtypes, as well as differential downstream targets. Thus, the claustrum comprises excitatory neuron subtypes with distinct molecular and projection properties, whose spatial patterns reflect the narrower and broader claustral extents debated in previous research. This subtype-specific heterogeneity likely shapes the functional complexity of the claustrum. eLife Sciences Publications, Ltd 2021-08-16 /pmc/articles/PMC8367382/ /pubmed/34397382 http://dx.doi.org/10.7554/eLife.68967 Text en © 2021, Erwin et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Erwin, Sarah R Bristow, Brianna N Sullivan, Kaitlin E Kendrick, Rennie M Marriott, Brian Wang, Lihua Clements, Jody Lemire, Andrew L Jackson, Jesse Cembrowski, Mark S Spatially patterned excitatory neuron subtypes and projections of the claustrum |
title | Spatially patterned excitatory neuron subtypes and projections of the claustrum |
title_full | Spatially patterned excitatory neuron subtypes and projections of the claustrum |
title_fullStr | Spatially patterned excitatory neuron subtypes and projections of the claustrum |
title_full_unstemmed | Spatially patterned excitatory neuron subtypes and projections of the claustrum |
title_short | Spatially patterned excitatory neuron subtypes and projections of the claustrum |
title_sort | spatially patterned excitatory neuron subtypes and projections of the claustrum |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8367382/ https://www.ncbi.nlm.nih.gov/pubmed/34397382 http://dx.doi.org/10.7554/eLife.68967 |
work_keys_str_mv | AT erwinsarahr spatiallypatternedexcitatoryneuronsubtypesandprojectionsoftheclaustrum AT bristowbriannan spatiallypatternedexcitatoryneuronsubtypesandprojectionsoftheclaustrum AT sullivankaitline spatiallypatternedexcitatoryneuronsubtypesandprojectionsoftheclaustrum AT kendrickrenniem spatiallypatternedexcitatoryneuronsubtypesandprojectionsoftheclaustrum AT marriottbrian spatiallypatternedexcitatoryneuronsubtypesandprojectionsoftheclaustrum AT wanglihua spatiallypatternedexcitatoryneuronsubtypesandprojectionsoftheclaustrum AT clementsjody spatiallypatternedexcitatoryneuronsubtypesandprojectionsoftheclaustrum AT lemireandrewl spatiallypatternedexcitatoryneuronsubtypesandprojectionsoftheclaustrum AT jacksonjesse spatiallypatternedexcitatoryneuronsubtypesandprojectionsoftheclaustrum AT cembrowskimarks spatiallypatternedexcitatoryneuronsubtypesandprojectionsoftheclaustrum |