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Spatially patterned excitatory neuron subtypes and projections of the claustrum

The claustrum is a functionally and structurally complex brain region, whose very spatial extent remains debated. Histochemical-based approaches typically treat the claustrum as a relatively narrow anatomical region that primarily projects to the neocortex, whereas circuit-based approaches can sugge...

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Autores principales: Erwin, Sarah R, Bristow, Brianna N, Sullivan, Kaitlin E, Kendrick, Rennie M, Marriott, Brian, Wang, Lihua, Clements, Jody, Lemire, Andrew L, Jackson, Jesse, Cembrowski, Mark S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8367382/
https://www.ncbi.nlm.nih.gov/pubmed/34397382
http://dx.doi.org/10.7554/eLife.68967
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author Erwin, Sarah R
Bristow, Brianna N
Sullivan, Kaitlin E
Kendrick, Rennie M
Marriott, Brian
Wang, Lihua
Clements, Jody
Lemire, Andrew L
Jackson, Jesse
Cembrowski, Mark S
author_facet Erwin, Sarah R
Bristow, Brianna N
Sullivan, Kaitlin E
Kendrick, Rennie M
Marriott, Brian
Wang, Lihua
Clements, Jody
Lemire, Andrew L
Jackson, Jesse
Cembrowski, Mark S
author_sort Erwin, Sarah R
collection PubMed
description The claustrum is a functionally and structurally complex brain region, whose very spatial extent remains debated. Histochemical-based approaches typically treat the claustrum as a relatively narrow anatomical region that primarily projects to the neocortex, whereas circuit-based approaches can suggest a broader claustrum region containing projections to the neocortex and other regions. Here, in the mouse, we took a bottom-up and cell-type-specific approach to complement and possibly unite these seemingly disparate conclusions. Using single-cell RNA-sequencing, we found that the claustrum comprises two excitatory neuron subtypes that are differentiable from the surrounding cortex. Multicolor retrograde tracing in conjunction with 12-channel multiplexed in situ hybridization revealed a core-shell spatial arrangement of these subtypes, as well as differential downstream targets. Thus, the claustrum comprises excitatory neuron subtypes with distinct molecular and projection properties, whose spatial patterns reflect the narrower and broader claustral extents debated in previous research. This subtype-specific heterogeneity likely shapes the functional complexity of the claustrum.
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spelling pubmed-83673822021-08-18 Spatially patterned excitatory neuron subtypes and projections of the claustrum Erwin, Sarah R Bristow, Brianna N Sullivan, Kaitlin E Kendrick, Rennie M Marriott, Brian Wang, Lihua Clements, Jody Lemire, Andrew L Jackson, Jesse Cembrowski, Mark S eLife Neuroscience The claustrum is a functionally and structurally complex brain region, whose very spatial extent remains debated. Histochemical-based approaches typically treat the claustrum as a relatively narrow anatomical region that primarily projects to the neocortex, whereas circuit-based approaches can suggest a broader claustrum region containing projections to the neocortex and other regions. Here, in the mouse, we took a bottom-up and cell-type-specific approach to complement and possibly unite these seemingly disparate conclusions. Using single-cell RNA-sequencing, we found that the claustrum comprises two excitatory neuron subtypes that are differentiable from the surrounding cortex. Multicolor retrograde tracing in conjunction with 12-channel multiplexed in situ hybridization revealed a core-shell spatial arrangement of these subtypes, as well as differential downstream targets. Thus, the claustrum comprises excitatory neuron subtypes with distinct molecular and projection properties, whose spatial patterns reflect the narrower and broader claustral extents debated in previous research. This subtype-specific heterogeneity likely shapes the functional complexity of the claustrum. eLife Sciences Publications, Ltd 2021-08-16 /pmc/articles/PMC8367382/ /pubmed/34397382 http://dx.doi.org/10.7554/eLife.68967 Text en © 2021, Erwin et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Erwin, Sarah R
Bristow, Brianna N
Sullivan, Kaitlin E
Kendrick, Rennie M
Marriott, Brian
Wang, Lihua
Clements, Jody
Lemire, Andrew L
Jackson, Jesse
Cembrowski, Mark S
Spatially patterned excitatory neuron subtypes and projections of the claustrum
title Spatially patterned excitatory neuron subtypes and projections of the claustrum
title_full Spatially patterned excitatory neuron subtypes and projections of the claustrum
title_fullStr Spatially patterned excitatory neuron subtypes and projections of the claustrum
title_full_unstemmed Spatially patterned excitatory neuron subtypes and projections of the claustrum
title_short Spatially patterned excitatory neuron subtypes and projections of the claustrum
title_sort spatially patterned excitatory neuron subtypes and projections of the claustrum
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8367382/
https://www.ncbi.nlm.nih.gov/pubmed/34397382
http://dx.doi.org/10.7554/eLife.68967
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