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The effects of zoledronic acid on the progression of experimental periodontitis in rats: histological and microtomographic analyses

PURPOSE: Periodontitis is considered a local risk factor for medication-related osteonecrosis of the jaws (MRONJ). However, little is known about the progression of periodontitis in the presence of zoledronic acid (ZOL). The aim of this study was to evaluate the effects of the systemic use of ZOL on...

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Autores principales: Leite de Marcelos, Priscylla Gonçalves Correia, Perez, Danyel Elias da Cruz Perez, Soares, Diego Moura, de Araújo, Samuel Silva, Evêncio, Liriane Baratella, Pontual, Maria Luiza dos Anjos, Ramos-Perez, Flávia Maria de Moraes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Academy of Periodontology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8367644/
https://www.ncbi.nlm.nih.gov/pubmed/34387046
http://dx.doi.org/10.5051/jpis.2001100055
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author Leite de Marcelos, Priscylla Gonçalves Correia
Perez, Danyel Elias da Cruz Perez
Soares, Diego Moura
de Araújo, Samuel Silva
Evêncio, Liriane Baratella
Pontual, Maria Luiza dos Anjos
Ramos-Perez, Flávia Maria de Moraes
author_facet Leite de Marcelos, Priscylla Gonçalves Correia
Perez, Danyel Elias da Cruz Perez
Soares, Diego Moura
de Araújo, Samuel Silva
Evêncio, Liriane Baratella
Pontual, Maria Luiza dos Anjos
Ramos-Perez, Flávia Maria de Moraes
author_sort Leite de Marcelos, Priscylla Gonçalves Correia
collection PubMed
description PURPOSE: Periodontitis is considered a local risk factor for medication-related osteonecrosis of the jaws (MRONJ). However, little is known about the progression of periodontitis in the presence of zoledronic acid (ZOL). The aim of this study was to evaluate the effects of the systemic use of ZOL on the progression of experimental periodontitis (EP) in rats, as ZOL could modulate the progression of periodontitis and concomitantly cause MRONJ in individuals with periodontitis. METHODS: Forty-eight male Wistar rats were randomly distributed in 6 groups (n=8 each). To induce EP, ligatures were placed around the right first mandibular molars. Three groups were treated with ZOL (0.15 mg/kg/week, intraperitoneal), and 3 with 0.9% saline solution (controls). In the ZOL/Lig30 and ZOL/Lig 15 groups, after 4 weeks of treatment with ZOL, EP was induced and euthanasia was performed after 30 and 15 days of EP induction, respectively. In both groups, the animals continued to receive ZOL after EP until the end of the experiment. In the Lig/ZOL group, EP was induced first, and 15 days later, ZOL was administered for 8 weeks, with euthanasia 1 week after the last dose. After euthanasia, the mandibles were evaluated using micro-computed microtomography (micro-CT) and histomorphometry. Bone loss was measured, and the presence of osteonecrosis was evaluated histologically. The data were evaluated using the Student t-test and the Mann-Whitney test, with a significance level of 5%. RESULTS: In the Lig/ZOL group, micro-CT revealed less alveolar bone resorption in the distal root (P<0.01) than in the control group (Lig/Con). Histomorphometric analysis confirmed less alveolar bone resorption in the Lig/ZOL group (P=0.001). Histologically, osteonecrosis was more common in the ZOL groups. CONCLUSION: ZOL decreased alveolar bone resorption in rats with EP. However, it presented a higher risk for MRONJ.
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spelling pubmed-83676442021-08-25 The effects of zoledronic acid on the progression of experimental periodontitis in rats: histological and microtomographic analyses Leite de Marcelos, Priscylla Gonçalves Correia Perez, Danyel Elias da Cruz Perez Soares, Diego Moura de Araújo, Samuel Silva Evêncio, Liriane Baratella Pontual, Maria Luiza dos Anjos Ramos-Perez, Flávia Maria de Moraes J Periodontal Implant Sci Research Article PURPOSE: Periodontitis is considered a local risk factor for medication-related osteonecrosis of the jaws (MRONJ). However, little is known about the progression of periodontitis in the presence of zoledronic acid (ZOL). The aim of this study was to evaluate the effects of the systemic use of ZOL on the progression of experimental periodontitis (EP) in rats, as ZOL could modulate the progression of periodontitis and concomitantly cause MRONJ in individuals with periodontitis. METHODS: Forty-eight male Wistar rats were randomly distributed in 6 groups (n=8 each). To induce EP, ligatures were placed around the right first mandibular molars. Three groups were treated with ZOL (0.15 mg/kg/week, intraperitoneal), and 3 with 0.9% saline solution (controls). In the ZOL/Lig30 and ZOL/Lig 15 groups, after 4 weeks of treatment with ZOL, EP was induced and euthanasia was performed after 30 and 15 days of EP induction, respectively. In both groups, the animals continued to receive ZOL after EP until the end of the experiment. In the Lig/ZOL group, EP was induced first, and 15 days later, ZOL was administered for 8 weeks, with euthanasia 1 week after the last dose. After euthanasia, the mandibles were evaluated using micro-computed microtomography (micro-CT) and histomorphometry. Bone loss was measured, and the presence of osteonecrosis was evaluated histologically. The data were evaluated using the Student t-test and the Mann-Whitney test, with a significance level of 5%. RESULTS: In the Lig/ZOL group, micro-CT revealed less alveolar bone resorption in the distal root (P<0.01) than in the control group (Lig/Con). Histomorphometric analysis confirmed less alveolar bone resorption in the Lig/ZOL group (P=0.001). Histologically, osteonecrosis was more common in the ZOL groups. CONCLUSION: ZOL decreased alveolar bone resorption in rats with EP. However, it presented a higher risk for MRONJ. Korean Academy of Periodontology 2021-05-07 /pmc/articles/PMC8367644/ /pubmed/34387046 http://dx.doi.org/10.5051/jpis.2001100055 Text en Copyright © 2021. Korean Academy of Periodontology https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/).
spellingShingle Research Article
Leite de Marcelos, Priscylla Gonçalves Correia
Perez, Danyel Elias da Cruz Perez
Soares, Diego Moura
de Araújo, Samuel Silva
Evêncio, Liriane Baratella
Pontual, Maria Luiza dos Anjos
Ramos-Perez, Flávia Maria de Moraes
The effects of zoledronic acid on the progression of experimental periodontitis in rats: histological and microtomographic analyses
title The effects of zoledronic acid on the progression of experimental periodontitis in rats: histological and microtomographic analyses
title_full The effects of zoledronic acid on the progression of experimental periodontitis in rats: histological and microtomographic analyses
title_fullStr The effects of zoledronic acid on the progression of experimental periodontitis in rats: histological and microtomographic analyses
title_full_unstemmed The effects of zoledronic acid on the progression of experimental periodontitis in rats: histological and microtomographic analyses
title_short The effects of zoledronic acid on the progression of experimental periodontitis in rats: histological and microtomographic analyses
title_sort effects of zoledronic acid on the progression of experimental periodontitis in rats: histological and microtomographic analyses
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8367644/
https://www.ncbi.nlm.nih.gov/pubmed/34387046
http://dx.doi.org/10.5051/jpis.2001100055
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