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Fluorogenic in vitro activity assay for the main protease M(pro) from SARS-CoV-2 and its adaptation to the identification of inhibitors

This protocol describes an in vitro fluorogenic assay to measure the proteolytic activity and identify inhibitors of M(pro), the main protease produced by SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2). Studies to identify potential inhibitors of M(pro) mainly rely on in silico molecul...

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Detalles Bibliográficos
Autores principales:	Ihssen, Julien, Faccio, Greta, Yao, Chunyan, Sirec, Teja, Spitz, Urs
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8367757/ https://www.ncbi.nlm.nih.gov/pubmed/34423318 http://dx.doi.org/10.1016/j.xpro.2021.100793

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