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Proteome Characterization of Glaucoma Aqueous Humor
Glaucoma is the leading cause of irreversible blindness worldwide. The proteome characterization of glaucoma is not clearly understood. A total of 175 subjects, including 57 primary acute angle-closure glaucoma (PAACG), 50 primary chronic angle-closure glaucoma (PCACG), 35 neovascular glaucoma (NVG)...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Biochemistry and Molecular Biology
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8367844/ https://www.ncbi.nlm.nih.gov/pubmed/34214668 http://dx.doi.org/10.1016/j.mcpro.2021.100117 |
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author | Liu, Xiaoyan Liu, Xiang Wang, Ying Sun, Haidan Guo, Zhengguang Tang, Xiaoyue Li, Jing Xiao, Xiaolian Zheng, Shuxin Yu, Mengxi He, Chengyan Xu, Jiyu Sun, Wei |
author_facet | Liu, Xiaoyan Liu, Xiang Wang, Ying Sun, Haidan Guo, Zhengguang Tang, Xiaoyue Li, Jing Xiao, Xiaolian Zheng, Shuxin Yu, Mengxi He, Chengyan Xu, Jiyu Sun, Wei |
author_sort | Liu, Xiaoyan |
collection | PubMed |
description | Glaucoma is the leading cause of irreversible blindness worldwide. The proteome characterization of glaucoma is not clearly understood. A total of 175 subjects, including 57 primary acute angle-closure glaucoma (PAACG), 50 primary chronic angle-closure glaucoma (PCACG), 35 neovascular glaucoma (NVG), and 33 cataract patients, were enrolled and comparison proteomic analysis was provided. The samples were randomly divided into discovery group or validation group, whose aqueous humor proteome was analyzed by data-independent acquisition or by parallel reaction monitoring. The common proteome features of three types of glaucoma were immune response, lipid metabolism, and cell death. Three proteins, VTN, SERPIND1, and CD14, showed significant upregulation in glaucoma and could discriminate glaucoma from cataract. Mutual differential proteomic analysis of PAACG, PCACG, and NVG showed different proteome characterization of the three types of glaucoma. NVG was characterized with activated angiogenesis. PAACG was characterized with activation of inflammation response. SERPIND1 was discovered to play vital role in glaucoma occurrences, which is associated with eye transparency decrease and glucose metabolism. This study would provide insights in understanding proteome characterization of glaucoma and benefit the clinical application of AH proteome. |
format | Online Article Text |
id | pubmed-8367844 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-83678442021-08-23 Proteome Characterization of Glaucoma Aqueous Humor Liu, Xiaoyan Liu, Xiang Wang, Ying Sun, Haidan Guo, Zhengguang Tang, Xiaoyue Li, Jing Xiao, Xiaolian Zheng, Shuxin Yu, Mengxi He, Chengyan Xu, Jiyu Sun, Wei Mol Cell Proteomics Research Glaucoma is the leading cause of irreversible blindness worldwide. The proteome characterization of glaucoma is not clearly understood. A total of 175 subjects, including 57 primary acute angle-closure glaucoma (PAACG), 50 primary chronic angle-closure glaucoma (PCACG), 35 neovascular glaucoma (NVG), and 33 cataract patients, were enrolled and comparison proteomic analysis was provided. The samples were randomly divided into discovery group or validation group, whose aqueous humor proteome was analyzed by data-independent acquisition or by parallel reaction monitoring. The common proteome features of three types of glaucoma were immune response, lipid metabolism, and cell death. Three proteins, VTN, SERPIND1, and CD14, showed significant upregulation in glaucoma and could discriminate glaucoma from cataract. Mutual differential proteomic analysis of PAACG, PCACG, and NVG showed different proteome characterization of the three types of glaucoma. NVG was characterized with activated angiogenesis. PAACG was characterized with activation of inflammation response. SERPIND1 was discovered to play vital role in glaucoma occurrences, which is associated with eye transparency decrease and glucose metabolism. This study would provide insights in understanding proteome characterization of glaucoma and benefit the clinical application of AH proteome. American Society for Biochemistry and Molecular Biology 2021-06-30 /pmc/articles/PMC8367844/ /pubmed/34214668 http://dx.doi.org/10.1016/j.mcpro.2021.100117 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Liu, Xiaoyan Liu, Xiang Wang, Ying Sun, Haidan Guo, Zhengguang Tang, Xiaoyue Li, Jing Xiao, Xiaolian Zheng, Shuxin Yu, Mengxi He, Chengyan Xu, Jiyu Sun, Wei Proteome Characterization of Glaucoma Aqueous Humor |
title | Proteome Characterization of Glaucoma Aqueous Humor |
title_full | Proteome Characterization of Glaucoma Aqueous Humor |
title_fullStr | Proteome Characterization of Glaucoma Aqueous Humor |
title_full_unstemmed | Proteome Characterization of Glaucoma Aqueous Humor |
title_short | Proteome Characterization of Glaucoma Aqueous Humor |
title_sort | proteome characterization of glaucoma aqueous humor |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8367844/ https://www.ncbi.nlm.nih.gov/pubmed/34214668 http://dx.doi.org/10.1016/j.mcpro.2021.100117 |
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