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The selection of naturally stable candidate foot-and-mouth disease virus vaccine strains for East Africa

Foot-and-mouth disease (FMD) is a global burden on the livestock industry. The causative agent, FMD virus (FMDV), is highly infectious and exists in seven distinct serotypes. Vaccination remains the most effective control strategy in endemic regions and current FMD vaccines are made from inactivated...

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Autores principales: Jackson, Ben, Harvey, Yongjie, Perez-Martin, Eva, Wilsden, Ginette, Juleff, Nicholas, Charleston, Bryan, Seago, Julian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8367848/
https://www.ncbi.nlm.nih.gov/pubmed/34303562
http://dx.doi.org/10.1016/j.vaccine.2021.07.001
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author Jackson, Ben
Harvey, Yongjie
Perez-Martin, Eva
Wilsden, Ginette
Juleff, Nicholas
Charleston, Bryan
Seago, Julian
author_facet Jackson, Ben
Harvey, Yongjie
Perez-Martin, Eva
Wilsden, Ginette
Juleff, Nicholas
Charleston, Bryan
Seago, Julian
author_sort Jackson, Ben
collection PubMed
description Foot-and-mouth disease (FMD) is a global burden on the livestock industry. The causative agent, FMD virus (FMDV), is highly infectious and exists in seven distinct serotypes. Vaccination remains the most effective control strategy in endemic regions and current FMD vaccines are made from inactivated preparations of whole virus. The inherent instability of FMDV and the emergence of new strains presents challenges to efficacious vaccine development. Currently, vaccines available in East Africa are comprised of relatively historic strains with unreported stabilities. As an initial step to produce an improved multivalent FMD vaccine we have identified naturally stable East African FMDV strains for each of the A, O, SAT1 and SAT2 serotypes and investigated their potential for protecting ruminants against strains that have recently circulated in East Africa. Interestingly, high diversity in stability between and within serotypes was observed, and in comparison to non-African A serotype viruses reported to date, the East African strains tested in this study are less stable. Candidate vaccine strains were adapted to propagation in BHK-21 cells with minimal capsid changes and used to generate vaccinate sera that effectively neutralised a panel of FMDV strains selected to improve FMD vaccines used in East Africa. This work highlights the importance of combining tools to predict and assess FMDV vaccine stability, with cell culture adaptation and serological tests in the development of FMD vaccines.
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spelling pubmed-83678482021-08-23 The selection of naturally stable candidate foot-and-mouth disease virus vaccine strains for East Africa Jackson, Ben Harvey, Yongjie Perez-Martin, Eva Wilsden, Ginette Juleff, Nicholas Charleston, Bryan Seago, Julian Vaccine Article Foot-and-mouth disease (FMD) is a global burden on the livestock industry. The causative agent, FMD virus (FMDV), is highly infectious and exists in seven distinct serotypes. Vaccination remains the most effective control strategy in endemic regions and current FMD vaccines are made from inactivated preparations of whole virus. The inherent instability of FMDV and the emergence of new strains presents challenges to efficacious vaccine development. Currently, vaccines available in East Africa are comprised of relatively historic strains with unreported stabilities. As an initial step to produce an improved multivalent FMD vaccine we have identified naturally stable East African FMDV strains for each of the A, O, SAT1 and SAT2 serotypes and investigated their potential for protecting ruminants against strains that have recently circulated in East Africa. Interestingly, high diversity in stability between and within serotypes was observed, and in comparison to non-African A serotype viruses reported to date, the East African strains tested in this study are less stable. Candidate vaccine strains were adapted to propagation in BHK-21 cells with minimal capsid changes and used to generate vaccinate sera that effectively neutralised a panel of FMDV strains selected to improve FMD vaccines used in East Africa. This work highlights the importance of combining tools to predict and assess FMDV vaccine stability, with cell culture adaptation and serological tests in the development of FMD vaccines. Elsevier Science 2021-08-16 /pmc/articles/PMC8367848/ /pubmed/34303562 http://dx.doi.org/10.1016/j.vaccine.2021.07.001 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jackson, Ben
Harvey, Yongjie
Perez-Martin, Eva
Wilsden, Ginette
Juleff, Nicholas
Charleston, Bryan
Seago, Julian
The selection of naturally stable candidate foot-and-mouth disease virus vaccine strains for East Africa
title The selection of naturally stable candidate foot-and-mouth disease virus vaccine strains for East Africa
title_full The selection of naturally stable candidate foot-and-mouth disease virus vaccine strains for East Africa
title_fullStr The selection of naturally stable candidate foot-and-mouth disease virus vaccine strains for East Africa
title_full_unstemmed The selection of naturally stable candidate foot-and-mouth disease virus vaccine strains for East Africa
title_short The selection of naturally stable candidate foot-and-mouth disease virus vaccine strains for East Africa
title_sort selection of naturally stable candidate foot-and-mouth disease virus vaccine strains for east africa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8367848/
https://www.ncbi.nlm.nih.gov/pubmed/34303562
http://dx.doi.org/10.1016/j.vaccine.2021.07.001
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