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Complete genome analysis of African swine fever virus responsible for outbreaks in domestic pigs in 2018 in Burundi and 2019 in Malawi

Several African swine fever (ASF) outbreaks in domestic pigs have been reported in Burundi and Malawi and whole-genome sequences of circulating outbreak viruses in these countries are limited. In the present study, complete genome sequences of ASF viruses (ASFV) that caused the 2018 outbreak in Buru...

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Autores principales: Hakizimana, Jean N., Ntirandekura, Jean B., Yona, Clara, Nyabongo, Lionel, Kamwendo, Gladson, Chulu, Julius L. C., Ntakirutimana, Désiré, Kamana, Olivier, Nauwynck, Hans, Misinzo, Gerald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8368048/
https://www.ncbi.nlm.nih.gov/pubmed/34402985
http://dx.doi.org/10.1007/s11250-021-02877-y
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author Hakizimana, Jean N.
Ntirandekura, Jean B.
Yona, Clara
Nyabongo, Lionel
Kamwendo, Gladson
Chulu, Julius L. C.
Ntakirutimana, Désiré
Kamana, Olivier
Nauwynck, Hans
Misinzo, Gerald
author_facet Hakizimana, Jean N.
Ntirandekura, Jean B.
Yona, Clara
Nyabongo, Lionel
Kamwendo, Gladson
Chulu, Julius L. C.
Ntakirutimana, Désiré
Kamana, Olivier
Nauwynck, Hans
Misinzo, Gerald
author_sort Hakizimana, Jean N.
collection PubMed
description Several African swine fever (ASF) outbreaks in domestic pigs have been reported in Burundi and Malawi and whole-genome sequences of circulating outbreak viruses in these countries are limited. In the present study, complete genome sequences of ASF viruses (ASFV) that caused the 2018 outbreak in Burundi (BUR/18/Rutana) and the 2019 outbreak in Malawi (MAL/19/Karonga) were produced using Illumina next-generation sequencing (NGS) platform and compared with other previously described ASFV complete genomes. The complete nucleotide sequences of BUR/18/Rutana and MAL/19/Karonga were 176,564 and 183,325 base pairs long with GC content of 38.62 and 38.48%, respectively. The MAL/19/Karonga virus had a total of 186 open reading frames (ORFs) while the BUR/18/Rutana strain had 151 ORFs. After comparative genomic analysis, the MAL/19/Karonga virus showed greater than 99% nucleotide identity with other complete nucleotides sequences of p72 genotype II viruses previously described in Tanzania, Europe and Asia including the Georgia 2007/1 isolate. The Burundian ASFV BUR/18/Rutana exhibited 98.95 to 99.34% nucleotide identity with genotype X ASFV previously described in Kenya and in Democratic Republic of the Congo (DRC). The serotyping results classified the BUR/18/Rutana and MAL/19/Karonga ASFV strains in serogroups 7 and 8, respectively. The results of this study provide insight into the genetic structure and antigenic diversity of ASFV strains circulating in Burundi and Malawi. This is important in order to understand the transmission dynamics and genetic evolution of ASFV in eastern Africa, with an ultimate goal of designing an efficient risk management strategy against ASF transboundary spread.
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spelling pubmed-83680482021-08-17 Complete genome analysis of African swine fever virus responsible for outbreaks in domestic pigs in 2018 in Burundi and 2019 in Malawi Hakizimana, Jean N. Ntirandekura, Jean B. Yona, Clara Nyabongo, Lionel Kamwendo, Gladson Chulu, Julius L. C. Ntakirutimana, Désiré Kamana, Olivier Nauwynck, Hans Misinzo, Gerald Trop Anim Health Prod Regular Articles Several African swine fever (ASF) outbreaks in domestic pigs have been reported in Burundi and Malawi and whole-genome sequences of circulating outbreak viruses in these countries are limited. In the present study, complete genome sequences of ASF viruses (ASFV) that caused the 2018 outbreak in Burundi (BUR/18/Rutana) and the 2019 outbreak in Malawi (MAL/19/Karonga) were produced using Illumina next-generation sequencing (NGS) platform and compared with other previously described ASFV complete genomes. The complete nucleotide sequences of BUR/18/Rutana and MAL/19/Karonga were 176,564 and 183,325 base pairs long with GC content of 38.62 and 38.48%, respectively. The MAL/19/Karonga virus had a total of 186 open reading frames (ORFs) while the BUR/18/Rutana strain had 151 ORFs. After comparative genomic analysis, the MAL/19/Karonga virus showed greater than 99% nucleotide identity with other complete nucleotides sequences of p72 genotype II viruses previously described in Tanzania, Europe and Asia including the Georgia 2007/1 isolate. The Burundian ASFV BUR/18/Rutana exhibited 98.95 to 99.34% nucleotide identity with genotype X ASFV previously described in Kenya and in Democratic Republic of the Congo (DRC). The serotyping results classified the BUR/18/Rutana and MAL/19/Karonga ASFV strains in serogroups 7 and 8, respectively. The results of this study provide insight into the genetic structure and antigenic diversity of ASFV strains circulating in Burundi and Malawi. This is important in order to understand the transmission dynamics and genetic evolution of ASFV in eastern Africa, with an ultimate goal of designing an efficient risk management strategy against ASF transboundary spread. Springer Netherlands 2021-08-17 2021 /pmc/articles/PMC8368048/ /pubmed/34402985 http://dx.doi.org/10.1007/s11250-021-02877-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Regular Articles
Hakizimana, Jean N.
Ntirandekura, Jean B.
Yona, Clara
Nyabongo, Lionel
Kamwendo, Gladson
Chulu, Julius L. C.
Ntakirutimana, Désiré
Kamana, Olivier
Nauwynck, Hans
Misinzo, Gerald
Complete genome analysis of African swine fever virus responsible for outbreaks in domestic pigs in 2018 in Burundi and 2019 in Malawi
title Complete genome analysis of African swine fever virus responsible for outbreaks in domestic pigs in 2018 in Burundi and 2019 in Malawi
title_full Complete genome analysis of African swine fever virus responsible for outbreaks in domestic pigs in 2018 in Burundi and 2019 in Malawi
title_fullStr Complete genome analysis of African swine fever virus responsible for outbreaks in domestic pigs in 2018 in Burundi and 2019 in Malawi
title_full_unstemmed Complete genome analysis of African swine fever virus responsible for outbreaks in domestic pigs in 2018 in Burundi and 2019 in Malawi
title_short Complete genome analysis of African swine fever virus responsible for outbreaks in domestic pigs in 2018 in Burundi and 2019 in Malawi
title_sort complete genome analysis of african swine fever virus responsible for outbreaks in domestic pigs in 2018 in burundi and 2019 in malawi
topic Regular Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8368048/
https://www.ncbi.nlm.nih.gov/pubmed/34402985
http://dx.doi.org/10.1007/s11250-021-02877-y
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