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Phase separation of Epstein-Barr virus EBNA2 protein reorganizes chromatin topology for epigenetic regulation
Epstein-Barr virus nuclear antigen 2 (EBNA2) is a transactivator of viral and cellular gene expression, which plays a critical role in the Epstein-Barr virus-associated diseases. It was reported that EBNA2 regulates gene expression by reorganizing chromatin and manipulating epigenetics. Recent studi...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8368186/ https://www.ncbi.nlm.nih.gov/pubmed/34400762 http://dx.doi.org/10.1038/s42003-021-02501-7 |
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author | Yang, Yiting Ye, Xidong Dai, Ranran Li, Zhaoqiang Zhang, Yan Xue, Wei Zhu, Yongchang Feng, Delong Qin, Litao Wang, Xin Lei, Bo Liao, Shixiu Hao, Bingtao |
author_facet | Yang, Yiting Ye, Xidong Dai, Ranran Li, Zhaoqiang Zhang, Yan Xue, Wei Zhu, Yongchang Feng, Delong Qin, Litao Wang, Xin Lei, Bo Liao, Shixiu Hao, Bingtao |
author_sort | Yang, Yiting |
collection | PubMed |
description | Epstein-Barr virus nuclear antigen 2 (EBNA2) is a transactivator of viral and cellular gene expression, which plays a critical role in the Epstein-Barr virus-associated diseases. It was reported that EBNA2 regulates gene expression by reorganizing chromatin and manipulating epigenetics. Recent studies showed that liquid-liquid phase separation plays an essential role in epigenetic and transcriptional regulation. Here we show that EBNA2 reorganized chromatin topology to form accessible chromatin domains (ACDs) of the host genome by phase separation. The N-terminal region of EBNA2, which is necessary for phase separation, is sufficient to induce ACDs. The C-terminal domain of EBNA2 promotes the acetylation of accessible chromatin regions by recruiting histone acetylase p300 to ACDs. According to these observations, we proposed a model of EBNA2 reorganizing chromatin topology for its acetylation through phase separation to explain the mechanism of EBNA2 hijacking the host genome by controlling its epigenetics. |
format | Online Article Text |
id | pubmed-8368186 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-83681862021-09-02 Phase separation of Epstein-Barr virus EBNA2 protein reorganizes chromatin topology for epigenetic regulation Yang, Yiting Ye, Xidong Dai, Ranran Li, Zhaoqiang Zhang, Yan Xue, Wei Zhu, Yongchang Feng, Delong Qin, Litao Wang, Xin Lei, Bo Liao, Shixiu Hao, Bingtao Commun Biol Article Epstein-Barr virus nuclear antigen 2 (EBNA2) is a transactivator of viral and cellular gene expression, which plays a critical role in the Epstein-Barr virus-associated diseases. It was reported that EBNA2 regulates gene expression by reorganizing chromatin and manipulating epigenetics. Recent studies showed that liquid-liquid phase separation plays an essential role in epigenetic and transcriptional regulation. Here we show that EBNA2 reorganized chromatin topology to form accessible chromatin domains (ACDs) of the host genome by phase separation. The N-terminal region of EBNA2, which is necessary for phase separation, is sufficient to induce ACDs. The C-terminal domain of EBNA2 promotes the acetylation of accessible chromatin regions by recruiting histone acetylase p300 to ACDs. According to these observations, we proposed a model of EBNA2 reorganizing chromatin topology for its acetylation through phase separation to explain the mechanism of EBNA2 hijacking the host genome by controlling its epigenetics. Nature Publishing Group UK 2021-08-16 /pmc/articles/PMC8368186/ /pubmed/34400762 http://dx.doi.org/10.1038/s42003-021-02501-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Yang, Yiting Ye, Xidong Dai, Ranran Li, Zhaoqiang Zhang, Yan Xue, Wei Zhu, Yongchang Feng, Delong Qin, Litao Wang, Xin Lei, Bo Liao, Shixiu Hao, Bingtao Phase separation of Epstein-Barr virus EBNA2 protein reorganizes chromatin topology for epigenetic regulation |
title | Phase separation of Epstein-Barr virus EBNA2 protein reorganizes chromatin topology for epigenetic regulation |
title_full | Phase separation of Epstein-Barr virus EBNA2 protein reorganizes chromatin topology for epigenetic regulation |
title_fullStr | Phase separation of Epstein-Barr virus EBNA2 protein reorganizes chromatin topology for epigenetic regulation |
title_full_unstemmed | Phase separation of Epstein-Barr virus EBNA2 protein reorganizes chromatin topology for epigenetic regulation |
title_short | Phase separation of Epstein-Barr virus EBNA2 protein reorganizes chromatin topology for epigenetic regulation |
title_sort | phase separation of epstein-barr virus ebna2 protein reorganizes chromatin topology for epigenetic regulation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8368186/ https://www.ncbi.nlm.nih.gov/pubmed/34400762 http://dx.doi.org/10.1038/s42003-021-02501-7 |
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