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Production of R- and S-1,2-propanediol in engineered Lactococcus lactis
1,2-propanediol (1,2-PDO) is a versatile chemical used in multiple manufacturing processes. To date, some engineered and non-engineered microbes, such as Escherichia coli, Lactobacillus buchneri, and Clostridium thermosaccharolyticum, have been used to produce 1,2-PDO. In this study, we demonstrated...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Berlin Heidelberg
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8368392/ https://www.ncbi.nlm.nih.gov/pubmed/34398341 http://dx.doi.org/10.1186/s13568-021-01276-8 |
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author | Sato, Rintaro Ikeda, Motoyuki Tanaka, Tomonari Ohara, Hitomi Aso, Yuji |
author_facet | Sato, Rintaro Ikeda, Motoyuki Tanaka, Tomonari Ohara, Hitomi Aso, Yuji |
author_sort | Sato, Rintaro |
collection | PubMed |
description | 1,2-propanediol (1,2-PDO) is a versatile chemical used in multiple manufacturing processes. To date, some engineered and non-engineered microbes, such as Escherichia coli, Lactobacillus buchneri, and Clostridium thermosaccharolyticum, have been used to produce 1,2-PDO. In this study, we demonstrated the production of R- and S-1,2-PDO using engineered Lactococcus lactis. The L- and D-lactic acid-producing L. lactis strains NZ9000 and AH1 were transformed with the plasmid pNZ8048-ppy harboring pct, pduP, and yahK genes for 1,2-PDO biosynthesis, resulting in L. lactis LL1 and LL2, respectively. These engineered L. lactis produced S- and R-1,2-PDO at concentrations of 0.69 and 0.50 g/L with 94.4 and 78.0% ee optical purities, respectively, from 1% glucose after 72 h of cultivation. Both 1% mannitol and 1% gluconate were added instead of glucose to the culture of L. lactis LL1 to supply NADH and NADPH to the 1,2-PDO production pathway, resulting in 75% enhancement of S-1,2-PDO production. Production of S-1,2-PDO from 5% mannitol and 5% gluconate was demonstrated using L. lactis LL1 with a pH–stat approach. This resulted in S-1,2-PDO production at a concentration of 1.88 g/L after 96 h of cultivation. To our knowledge, this is the first report on the production of R- and S-1,2-PDO using engineered lactic acid bacteria. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13568-021-01276-8. |
format | Online Article Text |
id | pubmed-8368392 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-83683922021-08-31 Production of R- and S-1,2-propanediol in engineered Lactococcus lactis Sato, Rintaro Ikeda, Motoyuki Tanaka, Tomonari Ohara, Hitomi Aso, Yuji AMB Express Original Article 1,2-propanediol (1,2-PDO) is a versatile chemical used in multiple manufacturing processes. To date, some engineered and non-engineered microbes, such as Escherichia coli, Lactobacillus buchneri, and Clostridium thermosaccharolyticum, have been used to produce 1,2-PDO. In this study, we demonstrated the production of R- and S-1,2-PDO using engineered Lactococcus lactis. The L- and D-lactic acid-producing L. lactis strains NZ9000 and AH1 were transformed with the plasmid pNZ8048-ppy harboring pct, pduP, and yahK genes for 1,2-PDO biosynthesis, resulting in L. lactis LL1 and LL2, respectively. These engineered L. lactis produced S- and R-1,2-PDO at concentrations of 0.69 and 0.50 g/L with 94.4 and 78.0% ee optical purities, respectively, from 1% glucose after 72 h of cultivation. Both 1% mannitol and 1% gluconate were added instead of glucose to the culture of L. lactis LL1 to supply NADH and NADPH to the 1,2-PDO production pathway, resulting in 75% enhancement of S-1,2-PDO production. Production of S-1,2-PDO from 5% mannitol and 5% gluconate was demonstrated using L. lactis LL1 with a pH–stat approach. This resulted in S-1,2-PDO production at a concentration of 1.88 g/L after 96 h of cultivation. To our knowledge, this is the first report on the production of R- and S-1,2-PDO using engineered lactic acid bacteria. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13568-021-01276-8. Springer Berlin Heidelberg 2021-08-16 /pmc/articles/PMC8368392/ /pubmed/34398341 http://dx.doi.org/10.1186/s13568-021-01276-8 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Sato, Rintaro Ikeda, Motoyuki Tanaka, Tomonari Ohara, Hitomi Aso, Yuji Production of R- and S-1,2-propanediol in engineered Lactococcus lactis |
title | Production of R- and S-1,2-propanediol in engineered Lactococcus lactis |
title_full | Production of R- and S-1,2-propanediol in engineered Lactococcus lactis |
title_fullStr | Production of R- and S-1,2-propanediol in engineered Lactococcus lactis |
title_full_unstemmed | Production of R- and S-1,2-propanediol in engineered Lactococcus lactis |
title_short | Production of R- and S-1,2-propanediol in engineered Lactococcus lactis |
title_sort | production of r- and s-1,2-propanediol in engineered lactococcus lactis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8368392/ https://www.ncbi.nlm.nih.gov/pubmed/34398341 http://dx.doi.org/10.1186/s13568-021-01276-8 |
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