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Liquiritin from Radix Glycyrrhizae Protects Cardiac Mitochondria from Hypoxia/Reoxygenation Damage

AIMS: The purpose of this study was to evaluate the protective effect of liquiritin (LIQ) from Radix Glycyrrhizae on cardiac mitochondria against hypoxia/reoxygenation (HR) injury. METHODS: H9C2 cells were subject to the HR model. LIQ purified from Radix Glycyrrhizae (purity > 95%) was administra...

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Autores principales: Thu, Vu Thi, Yen, Ngo Thi Hai, Ly, Nguyen Thi Ha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8369190/
https://www.ncbi.nlm.nih.gov/pubmed/34413986
http://dx.doi.org/10.1155/2021/1857464
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author Thu, Vu Thi
Yen, Ngo Thi Hai
Ly, Nguyen Thi Ha
author_facet Thu, Vu Thi
Yen, Ngo Thi Hai
Ly, Nguyen Thi Ha
author_sort Thu, Vu Thi
collection PubMed
description AIMS: The purpose of this study was to evaluate the protective effect of liquiritin (LIQ) from Radix Glycyrrhizae on cardiac mitochondria against hypoxia/reoxygenation (HR) injury. METHODS: H9C2 cells were subject to the HR model. LIQ purified from Radix Glycyrrhizae (purity > 95%) was administrated to reoxygenation period. Cell viability, mitochondrial mass, mitochondrial membrane potential, reactive oxygen species, and mitochondrial Ca(2)⁺ level were then assessed by using Cell Counting kit-8 and suitable fluorescence probe kits. RESULTS: LIQ administration remarkably reduced the rate of HR damage via increasing H9C2 cell viability level and preserving mitochondria after HR. Particularly, 60 μM of LIQ posthypoxic treatment markedly reduced cell death in HR-subjected H9C2 cell groups (p < 0.05). Interestingly, posthypoxic treatment of LIQ significantly prevented the loss of mitochondrial membrane potential, the decrease in mitochondrial mass, the increase in reactive oxygen species production, and the elevation of mitochondrial Ca(2)⁺ level in HR-treated H9C2 cells. CONCLUSION: The present study provides for the first time the cardioprotective of LIQ posthypoxic treatment via reducing H9C2 cell death and protecting cardiac mitochondria against HR damage.
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spelling pubmed-83691902021-08-18 Liquiritin from Radix Glycyrrhizae Protects Cardiac Mitochondria from Hypoxia/Reoxygenation Damage Thu, Vu Thi Yen, Ngo Thi Hai Ly, Nguyen Thi Ha J Anal Methods Chem Research Article AIMS: The purpose of this study was to evaluate the protective effect of liquiritin (LIQ) from Radix Glycyrrhizae on cardiac mitochondria against hypoxia/reoxygenation (HR) injury. METHODS: H9C2 cells were subject to the HR model. LIQ purified from Radix Glycyrrhizae (purity > 95%) was administrated to reoxygenation period. Cell viability, mitochondrial mass, mitochondrial membrane potential, reactive oxygen species, and mitochondrial Ca(2)⁺ level were then assessed by using Cell Counting kit-8 and suitable fluorescence probe kits. RESULTS: LIQ administration remarkably reduced the rate of HR damage via increasing H9C2 cell viability level and preserving mitochondria after HR. Particularly, 60 μM of LIQ posthypoxic treatment markedly reduced cell death in HR-subjected H9C2 cell groups (p < 0.05). Interestingly, posthypoxic treatment of LIQ significantly prevented the loss of mitochondrial membrane potential, the decrease in mitochondrial mass, the increase in reactive oxygen species production, and the elevation of mitochondrial Ca(2)⁺ level in HR-treated H9C2 cells. CONCLUSION: The present study provides for the first time the cardioprotective of LIQ posthypoxic treatment via reducing H9C2 cell death and protecting cardiac mitochondria against HR damage. Hindawi 2021-08-06 /pmc/articles/PMC8369190/ /pubmed/34413986 http://dx.doi.org/10.1155/2021/1857464 Text en Copyright © 2021 Vu Thi Thu et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Thu, Vu Thi
Yen, Ngo Thi Hai
Ly, Nguyen Thi Ha
Liquiritin from Radix Glycyrrhizae Protects Cardiac Mitochondria from Hypoxia/Reoxygenation Damage
title Liquiritin from Radix Glycyrrhizae Protects Cardiac Mitochondria from Hypoxia/Reoxygenation Damage
title_full Liquiritin from Radix Glycyrrhizae Protects Cardiac Mitochondria from Hypoxia/Reoxygenation Damage
title_fullStr Liquiritin from Radix Glycyrrhizae Protects Cardiac Mitochondria from Hypoxia/Reoxygenation Damage
title_full_unstemmed Liquiritin from Radix Glycyrrhizae Protects Cardiac Mitochondria from Hypoxia/Reoxygenation Damage
title_short Liquiritin from Radix Glycyrrhizae Protects Cardiac Mitochondria from Hypoxia/Reoxygenation Damage
title_sort liquiritin from radix glycyrrhizae protects cardiac mitochondria from hypoxia/reoxygenation damage
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8369190/
https://www.ncbi.nlm.nih.gov/pubmed/34413986
http://dx.doi.org/10.1155/2021/1857464
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