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Immunohistochemical Characterisation of GLUT1, MMP3 and NRF2 in Osteosarcoma

Osteosarcoma (OSA) is an aggressive bone malignancy. Unlike many other malignancies, OSA outcomes have not improved in recent decades. One challenge to the development of better diagnostic and therapeutic methods for OSA has been the lack of well characterized experimental model systems. Spontaneous...

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Autores principales: Rutland, Catrin S., Cockcroft, James M., Lothion-Roy, Jennifer, Harris, Anna E., Jeyapalan, Jennie N., Simpson, Siobhan, Alibhai, Aziza, Bailey, Clara, Ballard-Reisch, Alyssa C., Rizvanov, Albert A., Dunning, Mark D., de Brot, Simone, Mongan, Nigel P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8369506/
https://www.ncbi.nlm.nih.gov/pubmed/34414229
http://dx.doi.org/10.3389/fvets.2021.704598
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author Rutland, Catrin S.
Cockcroft, James M.
Lothion-Roy, Jennifer
Harris, Anna E.
Jeyapalan, Jennie N.
Simpson, Siobhan
Alibhai, Aziza
Bailey, Clara
Ballard-Reisch, Alyssa C.
Rizvanov, Albert A.
Dunning, Mark D.
de Brot, Simone
Mongan, Nigel P.
author_facet Rutland, Catrin S.
Cockcroft, James M.
Lothion-Roy, Jennifer
Harris, Anna E.
Jeyapalan, Jennie N.
Simpson, Siobhan
Alibhai, Aziza
Bailey, Clara
Ballard-Reisch, Alyssa C.
Rizvanov, Albert A.
Dunning, Mark D.
de Brot, Simone
Mongan, Nigel P.
author_sort Rutland, Catrin S.
collection PubMed
description Osteosarcoma (OSA) is an aggressive bone malignancy. Unlike many other malignancies, OSA outcomes have not improved in recent decades. One challenge to the development of better diagnostic and therapeutic methods for OSA has been the lack of well characterized experimental model systems. Spontaneous OSA in dogs provides a good model for the disease seen in people and also remains an important veterinary clinical challenge. We recently used RNA sequencing and qRT-PCR to provide a detailed molecular characterization of OSA relative to non-malignant bone in dogs. We identified differential mRNA expression of the solute carrier family 2 member 1 (SLC2A1/GLUT1), matrix metallopeptidase 3 (MMP3) and nuclear factor erythroid 2–related factor 2 (NFE2L2/NRF2) genes in canine OSA tissue in comparison to paired non-tumor tissue. Our present work characterizes protein expression of GLUT1, MMP3 and NRF2 using immunohistochemistry. As these proteins affect key processes such as Wnt activation, heme biosynthesis, glucose transport, understanding their expression and the enriched pathways and gene ontologies enables us to further understand the potential molecular pathways and mechanisms involved in OSA. This study further supports spontaneous OSA in dogs as a model system to inform the development of new methods to diagnose and treat OSA in both dogs and people.
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spelling pubmed-83695062021-08-18 Immunohistochemical Characterisation of GLUT1, MMP3 and NRF2 in Osteosarcoma Rutland, Catrin S. Cockcroft, James M. Lothion-Roy, Jennifer Harris, Anna E. Jeyapalan, Jennie N. Simpson, Siobhan Alibhai, Aziza Bailey, Clara Ballard-Reisch, Alyssa C. Rizvanov, Albert A. Dunning, Mark D. de Brot, Simone Mongan, Nigel P. Front Vet Sci Veterinary Science Osteosarcoma (OSA) is an aggressive bone malignancy. Unlike many other malignancies, OSA outcomes have not improved in recent decades. One challenge to the development of better diagnostic and therapeutic methods for OSA has been the lack of well characterized experimental model systems. Spontaneous OSA in dogs provides a good model for the disease seen in people and also remains an important veterinary clinical challenge. We recently used RNA sequencing and qRT-PCR to provide a detailed molecular characterization of OSA relative to non-malignant bone in dogs. We identified differential mRNA expression of the solute carrier family 2 member 1 (SLC2A1/GLUT1), matrix metallopeptidase 3 (MMP3) and nuclear factor erythroid 2–related factor 2 (NFE2L2/NRF2) genes in canine OSA tissue in comparison to paired non-tumor tissue. Our present work characterizes protein expression of GLUT1, MMP3 and NRF2 using immunohistochemistry. As these proteins affect key processes such as Wnt activation, heme biosynthesis, glucose transport, understanding their expression and the enriched pathways and gene ontologies enables us to further understand the potential molecular pathways and mechanisms involved in OSA. This study further supports spontaneous OSA in dogs as a model system to inform the development of new methods to diagnose and treat OSA in both dogs and people. Frontiers Media S.A. 2021-08-03 /pmc/articles/PMC8369506/ /pubmed/34414229 http://dx.doi.org/10.3389/fvets.2021.704598 Text en Copyright © 2021 Rutland, Cockcroft, Lothion-Roy, Harris, Jeyapalan, Simpson, Alibhai, Bailey, Ballard-Reisch, Rizvanov, Dunning, de Brot and Mongan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Rutland, Catrin S.
Cockcroft, James M.
Lothion-Roy, Jennifer
Harris, Anna E.
Jeyapalan, Jennie N.
Simpson, Siobhan
Alibhai, Aziza
Bailey, Clara
Ballard-Reisch, Alyssa C.
Rizvanov, Albert A.
Dunning, Mark D.
de Brot, Simone
Mongan, Nigel P.
Immunohistochemical Characterisation of GLUT1, MMP3 and NRF2 in Osteosarcoma
title Immunohistochemical Characterisation of GLUT1, MMP3 and NRF2 in Osteosarcoma
title_full Immunohistochemical Characterisation of GLUT1, MMP3 and NRF2 in Osteosarcoma
title_fullStr Immunohistochemical Characterisation of GLUT1, MMP3 and NRF2 in Osteosarcoma
title_full_unstemmed Immunohistochemical Characterisation of GLUT1, MMP3 and NRF2 in Osteosarcoma
title_short Immunohistochemical Characterisation of GLUT1, MMP3 and NRF2 in Osteosarcoma
title_sort immunohistochemical characterisation of glut1, mmp3 and nrf2 in osteosarcoma
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8369506/
https://www.ncbi.nlm.nih.gov/pubmed/34414229
http://dx.doi.org/10.3389/fvets.2021.704598
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