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A detailed analysis of innate and adaptive immune responsiveness upon infection with Salmonella enterica serotype Enteritidis in young broiler chickens

Salmonella enterica serotype Enteritidis (SE) is a zoonotic pathogen which causes foodborne diseases in humans as well as severe disease symptoms in young chickens. More insight in innate and adaptive immune responses of chickens to SE infection is needed to understand elimination of SE. Seven-day-o...

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Autores principales: Meijerink, Nathalie, van den Biggelaar, Robin H. G. A., van Haarlem, Daphne A., Stegeman, J. Arjan, Rutten, Victor P. M. G., Jansen, Christine A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8369617/
https://www.ncbi.nlm.nih.gov/pubmed/34404469
http://dx.doi.org/10.1186/s13567-021-00978-y
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author Meijerink, Nathalie
van den Biggelaar, Robin H. G. A.
van Haarlem, Daphne A.
Stegeman, J. Arjan
Rutten, Victor P. M. G.
Jansen, Christine A.
author_facet Meijerink, Nathalie
van den Biggelaar, Robin H. G. A.
van Haarlem, Daphne A.
Stegeman, J. Arjan
Rutten, Victor P. M. G.
Jansen, Christine A.
author_sort Meijerink, Nathalie
collection PubMed
description Salmonella enterica serotype Enteritidis (SE) is a zoonotic pathogen which causes foodborne diseases in humans as well as severe disease symptoms in young chickens. More insight in innate and adaptive immune responses of chickens to SE infection is needed to understand elimination of SE. Seven-day-old broiler chickens were experimentally challenged with SE and numbers and responsiveness of innate and adaptive immune cells as well as antibody titers were assessed. SE was observed in the ileum and spleen of SE-infected chickens at 7 days post-infection (dpi). At 1 dpi numbers of intraepithelial cytotoxic CD8(+) T cells were significantly increased alongside numerically increased intraepithelial IL-2Rα(+) and 20E5(+) natural killer (NK) cells at 1 and 3 dpi. At both time points, activation of intraepithelial and splenic NK cells was significantly enhanced. At 7 dpi in the spleen, presence of macrophages and expression of activation markers on dendritic cells were significantly increased. At 21 dpi, SE-induced proliferation of splenic CD4(+) and CD8(+) T cells was observed and SE-specific antibodies were detected in sera of all SE-infected chickens. In conclusion, SE results in enhanced numbers and activation of innate cells and we hypothesized that in concert with subsequent specific T cell and antibody responses, reduction of SE is achieved. A better understanding of innate and adaptive immune responses important in the elimination of SE will aid in developing immune-modulation strategies, which may increase resistance to SE in young broiler chickens. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13567-021-00978-y.
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spelling pubmed-83696172021-08-18 A detailed analysis of innate and adaptive immune responsiveness upon infection with Salmonella enterica serotype Enteritidis in young broiler chickens Meijerink, Nathalie van den Biggelaar, Robin H. G. A. van Haarlem, Daphne A. Stegeman, J. Arjan Rutten, Victor P. M. G. Jansen, Christine A. Vet Res Research Article Salmonella enterica serotype Enteritidis (SE) is a zoonotic pathogen which causes foodborne diseases in humans as well as severe disease symptoms in young chickens. More insight in innate and adaptive immune responses of chickens to SE infection is needed to understand elimination of SE. Seven-day-old broiler chickens were experimentally challenged with SE and numbers and responsiveness of innate and adaptive immune cells as well as antibody titers were assessed. SE was observed in the ileum and spleen of SE-infected chickens at 7 days post-infection (dpi). At 1 dpi numbers of intraepithelial cytotoxic CD8(+) T cells were significantly increased alongside numerically increased intraepithelial IL-2Rα(+) and 20E5(+) natural killer (NK) cells at 1 and 3 dpi. At both time points, activation of intraepithelial and splenic NK cells was significantly enhanced. At 7 dpi in the spleen, presence of macrophages and expression of activation markers on dendritic cells were significantly increased. At 21 dpi, SE-induced proliferation of splenic CD4(+) and CD8(+) T cells was observed and SE-specific antibodies were detected in sera of all SE-infected chickens. In conclusion, SE results in enhanced numbers and activation of innate cells and we hypothesized that in concert with subsequent specific T cell and antibody responses, reduction of SE is achieved. A better understanding of innate and adaptive immune responses important in the elimination of SE will aid in developing immune-modulation strategies, which may increase resistance to SE in young broiler chickens. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13567-021-00978-y. BioMed Central 2021-08-17 2021 /pmc/articles/PMC8369617/ /pubmed/34404469 http://dx.doi.org/10.1186/s13567-021-00978-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Meijerink, Nathalie
van den Biggelaar, Robin H. G. A.
van Haarlem, Daphne A.
Stegeman, J. Arjan
Rutten, Victor P. M. G.
Jansen, Christine A.
A detailed analysis of innate and adaptive immune responsiveness upon infection with Salmonella enterica serotype Enteritidis in young broiler chickens
title A detailed analysis of innate and adaptive immune responsiveness upon infection with Salmonella enterica serotype Enteritidis in young broiler chickens
title_full A detailed analysis of innate and adaptive immune responsiveness upon infection with Salmonella enterica serotype Enteritidis in young broiler chickens
title_fullStr A detailed analysis of innate and adaptive immune responsiveness upon infection with Salmonella enterica serotype Enteritidis in young broiler chickens
title_full_unstemmed A detailed analysis of innate and adaptive immune responsiveness upon infection with Salmonella enterica serotype Enteritidis in young broiler chickens
title_short A detailed analysis of innate and adaptive immune responsiveness upon infection with Salmonella enterica serotype Enteritidis in young broiler chickens
title_sort detailed analysis of innate and adaptive immune responsiveness upon infection with salmonella enterica serotype enteritidis in young broiler chickens
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8369617/
https://www.ncbi.nlm.nih.gov/pubmed/34404469
http://dx.doi.org/10.1186/s13567-021-00978-y
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