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Purification and Characterization of a Thermo- and pH-Stable Laccase From the Litter-Decomposing Fungus Gymnopus luxurians and Laccase Mediator Systems for Dye Decolorization

An extracellular laccase (GLL) was purified from fermentation broth of the litter-decomposing fungus Gymnopus luxurians by four chromatography steps, which resulted in a high specific activity of 118.82 U/mg, purification fold of 41.22, and recovery rate of 42.05%. It is a monomeric protein with a m...

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Autores principales: Sun, Yue, Liu, Zi-Lu, Hu, Bo-Yang, Chen, Qing-Jun, Yang, Ai-Zhen, Wang, Qiu-Ying, Li, Xiao-Feng, Zhang, Jia-Yan, Zhang, Guo-Qing, Zhao, Yong-Chang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8369832/
https://www.ncbi.nlm.nih.gov/pubmed/34413835
http://dx.doi.org/10.3389/fmicb.2021.672620
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author Sun, Yue
Liu, Zi-Lu
Hu, Bo-Yang
Chen, Qing-Jun
Yang, Ai-Zhen
Wang, Qiu-Ying
Li, Xiao-Feng
Zhang, Jia-Yan
Zhang, Guo-Qing
Zhao, Yong-Chang
author_facet Sun, Yue
Liu, Zi-Lu
Hu, Bo-Yang
Chen, Qing-Jun
Yang, Ai-Zhen
Wang, Qiu-Ying
Li, Xiao-Feng
Zhang, Jia-Yan
Zhang, Guo-Qing
Zhao, Yong-Chang
author_sort Sun, Yue
collection PubMed
description An extracellular laccase (GLL) was purified from fermentation broth of the litter-decomposing fungus Gymnopus luxurians by four chromatography steps, which resulted in a high specific activity of 118.82 U/mg, purification fold of 41.22, and recovery rate of 42.05%. It is a monomeric protein with a molecular weight of 64 kDa and N-terminal amino acid sequence of AIGPV TDLHI, suggesting that GLL is a typical fungal laccase. GLL demonstrated an optimum temperature range of 55°C–65°C and an optimum pH 2.2 toward 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). It displayed considerably high thermostability and pH stability with about 63% activity retained after 24 h at 50°C, and 86% activity retained after 24 h at pH 2.2, respectively. GLL was significantly enhanced in the presence of K(+), Na(+), and Mg(2+) ions. It demonstrated K(m) of 539 μM and k(cat)/K(m) of 140 mM(–1)⋅s(–1) toward ABTS at pH 2.2 and 37°C. Acetosyringone (AS) and syringaldehyde (SA) were the optimal mediators of GLL (0.4 U/ml) for dye decolorization with decolorization rates of about 60%–90% toward 11 of the 14 synthetic dyes. The optimum reaction conditions were determined to be mediator concentration of 0.1 mM, temperature range of 25°C –60°C, and pH 4.0. The purified laccase was the first laccase isolated from genus Gymnopus with high thermostability, pH stability, and effective decolorization toward dyes, suggesting that it has potentials for textile and environmental applications.
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spelling pubmed-83698322021-08-18 Purification and Characterization of a Thermo- and pH-Stable Laccase From the Litter-Decomposing Fungus Gymnopus luxurians and Laccase Mediator Systems for Dye Decolorization Sun, Yue Liu, Zi-Lu Hu, Bo-Yang Chen, Qing-Jun Yang, Ai-Zhen Wang, Qiu-Ying Li, Xiao-Feng Zhang, Jia-Yan Zhang, Guo-Qing Zhao, Yong-Chang Front Microbiol Microbiology An extracellular laccase (GLL) was purified from fermentation broth of the litter-decomposing fungus Gymnopus luxurians by four chromatography steps, which resulted in a high specific activity of 118.82 U/mg, purification fold of 41.22, and recovery rate of 42.05%. It is a monomeric protein with a molecular weight of 64 kDa and N-terminal amino acid sequence of AIGPV TDLHI, suggesting that GLL is a typical fungal laccase. GLL demonstrated an optimum temperature range of 55°C–65°C and an optimum pH 2.2 toward 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). It displayed considerably high thermostability and pH stability with about 63% activity retained after 24 h at 50°C, and 86% activity retained after 24 h at pH 2.2, respectively. GLL was significantly enhanced in the presence of K(+), Na(+), and Mg(2+) ions. It demonstrated K(m) of 539 μM and k(cat)/K(m) of 140 mM(–1)⋅s(–1) toward ABTS at pH 2.2 and 37°C. Acetosyringone (AS) and syringaldehyde (SA) were the optimal mediators of GLL (0.4 U/ml) for dye decolorization with decolorization rates of about 60%–90% toward 11 of the 14 synthetic dyes. The optimum reaction conditions were determined to be mediator concentration of 0.1 mM, temperature range of 25°C –60°C, and pH 4.0. The purified laccase was the first laccase isolated from genus Gymnopus with high thermostability, pH stability, and effective decolorization toward dyes, suggesting that it has potentials for textile and environmental applications. Frontiers Media S.A. 2021-08-03 /pmc/articles/PMC8369832/ /pubmed/34413835 http://dx.doi.org/10.3389/fmicb.2021.672620 Text en Copyright © 2021 Sun, Liu, Hu, Chen, Yang, Wang, Li, Zhang, Zhang and Zhao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Sun, Yue
Liu, Zi-Lu
Hu, Bo-Yang
Chen, Qing-Jun
Yang, Ai-Zhen
Wang, Qiu-Ying
Li, Xiao-Feng
Zhang, Jia-Yan
Zhang, Guo-Qing
Zhao, Yong-Chang
Purification and Characterization of a Thermo- and pH-Stable Laccase From the Litter-Decomposing Fungus Gymnopus luxurians and Laccase Mediator Systems for Dye Decolorization
title Purification and Characterization of a Thermo- and pH-Stable Laccase From the Litter-Decomposing Fungus Gymnopus luxurians and Laccase Mediator Systems for Dye Decolorization
title_full Purification and Characterization of a Thermo- and pH-Stable Laccase From the Litter-Decomposing Fungus Gymnopus luxurians and Laccase Mediator Systems for Dye Decolorization
title_fullStr Purification and Characterization of a Thermo- and pH-Stable Laccase From the Litter-Decomposing Fungus Gymnopus luxurians and Laccase Mediator Systems for Dye Decolorization
title_full_unstemmed Purification and Characterization of a Thermo- and pH-Stable Laccase From the Litter-Decomposing Fungus Gymnopus luxurians and Laccase Mediator Systems for Dye Decolorization
title_short Purification and Characterization of a Thermo- and pH-Stable Laccase From the Litter-Decomposing Fungus Gymnopus luxurians and Laccase Mediator Systems for Dye Decolorization
title_sort purification and characterization of a thermo- and ph-stable laccase from the litter-decomposing fungus gymnopus luxurians and laccase mediator systems for dye decolorization
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8369832/
https://www.ncbi.nlm.nih.gov/pubmed/34413835
http://dx.doi.org/10.3389/fmicb.2021.672620
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