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The Small Protein YmoA Controls the Csr System and Adjusts Expression of Virulence-Relevant Traits of Yersinia pseudotuberculosis

Virulence gene expression of Yersinia pseudotuberculosis changes during the different stages of infection and this is tightly controlled by environmental cues. In this study, we show that the small protein YmoA, a member of the Hha family, is part of this process. It controls temperature- and nutrie...

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Autores principales: Böhme, Katja, Heroven, Ann Kathrin, Lobedann, Stephanie, Guo, Yuzhu, Stolle, Anne-Sophie, Dersch, Petra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8369931/
https://www.ncbi.nlm.nih.gov/pubmed/34413840
http://dx.doi.org/10.3389/fmicb.2021.706934
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author Böhme, Katja
Heroven, Ann Kathrin
Lobedann, Stephanie
Guo, Yuzhu
Stolle, Anne-Sophie
Dersch, Petra
author_facet Böhme, Katja
Heroven, Ann Kathrin
Lobedann, Stephanie
Guo, Yuzhu
Stolle, Anne-Sophie
Dersch, Petra
author_sort Böhme, Katja
collection PubMed
description Virulence gene expression of Yersinia pseudotuberculosis changes during the different stages of infection and this is tightly controlled by environmental cues. In this study, we show that the small protein YmoA, a member of the Hha family, is part of this process. It controls temperature- and nutrient-dependent early and later stage virulence genes in an opposing manner and co-regulates bacterial stress responses and metabolic functions. Our analysis further revealed that YmoA exerts this function by modulating the global post-transcriptional regulatory Csr system. YmoA pre-dominantly enhances the stability of the regulatory RNA CsrC. This involves a stabilizing stem-loop structure within the 5′-region of CsrC. YmoA-mediated CsrC stabilization depends on H-NS, but not on the RNA chaperone Hfq. YmoA-promoted reprogramming of the Csr system has severe consequences for the cell: we found that a mutant deficient of ymoA is strongly reduced in its ability to enter host cells and to disseminate to the Peyer’s patches, mesenteric lymph nodes, liver and spleen in mice. We propose a model in which YmoA controls transition from the initial colonization phase in the intestine toward the host defense phase important for the long-term establishment of the infection in underlying tissues.
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spelling pubmed-83699312021-08-18 The Small Protein YmoA Controls the Csr System and Adjusts Expression of Virulence-Relevant Traits of Yersinia pseudotuberculosis Böhme, Katja Heroven, Ann Kathrin Lobedann, Stephanie Guo, Yuzhu Stolle, Anne-Sophie Dersch, Petra Front Microbiol Microbiology Virulence gene expression of Yersinia pseudotuberculosis changes during the different stages of infection and this is tightly controlled by environmental cues. In this study, we show that the small protein YmoA, a member of the Hha family, is part of this process. It controls temperature- and nutrient-dependent early and later stage virulence genes in an opposing manner and co-regulates bacterial stress responses and metabolic functions. Our analysis further revealed that YmoA exerts this function by modulating the global post-transcriptional regulatory Csr system. YmoA pre-dominantly enhances the stability of the regulatory RNA CsrC. This involves a stabilizing stem-loop structure within the 5′-region of CsrC. YmoA-mediated CsrC stabilization depends on H-NS, but not on the RNA chaperone Hfq. YmoA-promoted reprogramming of the Csr system has severe consequences for the cell: we found that a mutant deficient of ymoA is strongly reduced in its ability to enter host cells and to disseminate to the Peyer’s patches, mesenteric lymph nodes, liver and spleen in mice. We propose a model in which YmoA controls transition from the initial colonization phase in the intestine toward the host defense phase important for the long-term establishment of the infection in underlying tissues. Frontiers Media S.A. 2021-08-03 /pmc/articles/PMC8369931/ /pubmed/34413840 http://dx.doi.org/10.3389/fmicb.2021.706934 Text en Copyright © 2021 Böhme, Heroven, Lobedann, Guo, Stolle and Dersch. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Böhme, Katja
Heroven, Ann Kathrin
Lobedann, Stephanie
Guo, Yuzhu
Stolle, Anne-Sophie
Dersch, Petra
The Small Protein YmoA Controls the Csr System and Adjusts Expression of Virulence-Relevant Traits of Yersinia pseudotuberculosis
title The Small Protein YmoA Controls the Csr System and Adjusts Expression of Virulence-Relevant Traits of Yersinia pseudotuberculosis
title_full The Small Protein YmoA Controls the Csr System and Adjusts Expression of Virulence-Relevant Traits of Yersinia pseudotuberculosis
title_fullStr The Small Protein YmoA Controls the Csr System and Adjusts Expression of Virulence-Relevant Traits of Yersinia pseudotuberculosis
title_full_unstemmed The Small Protein YmoA Controls the Csr System and Adjusts Expression of Virulence-Relevant Traits of Yersinia pseudotuberculosis
title_short The Small Protein YmoA Controls the Csr System and Adjusts Expression of Virulence-Relevant Traits of Yersinia pseudotuberculosis
title_sort small protein ymoa controls the csr system and adjusts expression of virulence-relevant traits of yersinia pseudotuberculosis
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8369931/
https://www.ncbi.nlm.nih.gov/pubmed/34413840
http://dx.doi.org/10.3389/fmicb.2021.706934
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