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Alterations of Vaginal Microbiota in Women With Infertility and Chlamydia trachomatis Infection

Chlamydia trachomatis (C. trachomatis) is the most common etiological agent of bacterial sexually transmitted infections (STIs) worldwide and causes serious health sequelae such as cervicitis, pelvic inflammatory disease, and even infertility if ascending from the lower to the upper female genital t...

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Autores principales: Chen, Hongliang, Wang, Li, Zhao, Lanhua, Luo, Lipei, Min, Shuling, Wen, Yating, Lei, Wenbo, Shu, Mingyi, Li, Zhongyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8370387/
https://www.ncbi.nlm.nih.gov/pubmed/34414130
http://dx.doi.org/10.3389/fcimb.2021.698840
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author Chen, Hongliang
Wang, Li
Zhao, Lanhua
Luo, Lipei
Min, Shuling
Wen, Yating
Lei, Wenbo
Shu, Mingyi
Li, Zhongyu
author_facet Chen, Hongliang
Wang, Li
Zhao, Lanhua
Luo, Lipei
Min, Shuling
Wen, Yating
Lei, Wenbo
Shu, Mingyi
Li, Zhongyu
author_sort Chen, Hongliang
collection PubMed
description Chlamydia trachomatis (C. trachomatis) is the most common etiological agent of bacterial sexually transmitted infections (STIs) worldwide and causes serious health sequelae such as cervicitis, pelvic inflammatory disease, and even infertility if ascending from the lower to the upper female genital tract. Previous studies have revealed the pivotal role of vaginal microbiota in susceptibility to STIs. However, alterations in the vaginal microbiota in women who are infertile and infected with C. trachomatis remain unknown. This study used metagenomic analysis of sequenced 16S rRNA gene amplicons to examine the vaginal microbial profiles of women with tubal infertility who were C. trachomatis-negative and those who were C. trachomatis-positive pre- and post-antibiotic treatment. Women who were C. trachomatis-negative and deemed healthy were recruited as references of eubiosis and dysbiosis. Women with tubal infertility and C. trachomatis infection presented a unique Lactobacillus iners-dominated vaginal microbiota rather than one dominated by Lactobacillus crispatus and displayed a decrease in Lactobacillus, Bifidobacterium, Enterobacter, Atopobium, and Streptococcus, accompanied by decreased levels of cytokines such as interferon (IFN)-γ and interleukin (IL)-10. This altered vaginal microbiota could be restored with varying degrees after standard treatment for C. trachomatis. This shift could be a predictive vaginal microbiota signature for C. trachomatis infection among females with tubal infertility, while no significant differences in phylum, class, and operational taxonomic unit (OTU) levels were observed between women with tubal infertility who were C. trachomatis-negative and healthy controls. This is the first study to provide data on the association of vaginal microbiota with C. trachomatis infection among women with tubal infertility and highlights unprecedented potential opportunities to predict C. trachomatis infection.
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spelling pubmed-83703872021-08-18 Alterations of Vaginal Microbiota in Women With Infertility and Chlamydia trachomatis Infection Chen, Hongliang Wang, Li Zhao, Lanhua Luo, Lipei Min, Shuling Wen, Yating Lei, Wenbo Shu, Mingyi Li, Zhongyu Front Cell Infect Microbiol Cellular and Infection Microbiology Chlamydia trachomatis (C. trachomatis) is the most common etiological agent of bacterial sexually transmitted infections (STIs) worldwide and causes serious health sequelae such as cervicitis, pelvic inflammatory disease, and even infertility if ascending from the lower to the upper female genital tract. Previous studies have revealed the pivotal role of vaginal microbiota in susceptibility to STIs. However, alterations in the vaginal microbiota in women who are infertile and infected with C. trachomatis remain unknown. This study used metagenomic analysis of sequenced 16S rRNA gene amplicons to examine the vaginal microbial profiles of women with tubal infertility who were C. trachomatis-negative and those who were C. trachomatis-positive pre- and post-antibiotic treatment. Women who were C. trachomatis-negative and deemed healthy were recruited as references of eubiosis and dysbiosis. Women with tubal infertility and C. trachomatis infection presented a unique Lactobacillus iners-dominated vaginal microbiota rather than one dominated by Lactobacillus crispatus and displayed a decrease in Lactobacillus, Bifidobacterium, Enterobacter, Atopobium, and Streptococcus, accompanied by decreased levels of cytokines such as interferon (IFN)-γ and interleukin (IL)-10. This altered vaginal microbiota could be restored with varying degrees after standard treatment for C. trachomatis. This shift could be a predictive vaginal microbiota signature for C. trachomatis infection among females with tubal infertility, while no significant differences in phylum, class, and operational taxonomic unit (OTU) levels were observed between women with tubal infertility who were C. trachomatis-negative and healthy controls. This is the first study to provide data on the association of vaginal microbiota with C. trachomatis infection among women with tubal infertility and highlights unprecedented potential opportunities to predict C. trachomatis infection. Frontiers Media S.A. 2021-08-03 /pmc/articles/PMC8370387/ /pubmed/34414130 http://dx.doi.org/10.3389/fcimb.2021.698840 Text en Copyright © 2021 Chen, Wang, Zhao, Luo, Min, Wen, Lei, Shu and Li https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Chen, Hongliang
Wang, Li
Zhao, Lanhua
Luo, Lipei
Min, Shuling
Wen, Yating
Lei, Wenbo
Shu, Mingyi
Li, Zhongyu
Alterations of Vaginal Microbiota in Women With Infertility and Chlamydia trachomatis Infection
title Alterations of Vaginal Microbiota in Women With Infertility and Chlamydia trachomatis Infection
title_full Alterations of Vaginal Microbiota in Women With Infertility and Chlamydia trachomatis Infection
title_fullStr Alterations of Vaginal Microbiota in Women With Infertility and Chlamydia trachomatis Infection
title_full_unstemmed Alterations of Vaginal Microbiota in Women With Infertility and Chlamydia trachomatis Infection
title_short Alterations of Vaginal Microbiota in Women With Infertility and Chlamydia trachomatis Infection
title_sort alterations of vaginal microbiota in women with infertility and chlamydia trachomatis infection
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8370387/
https://www.ncbi.nlm.nih.gov/pubmed/34414130
http://dx.doi.org/10.3389/fcimb.2021.698840
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